Identification of Mycobacterium pseudoshottsii in the Eastern Mediterranean
ABSTRACT Among the numerous pathogenic nontuberculous mycobacteria (NTM), which may cause disease in both poikilothermic and homoeothermic organisms, members of the unique clade Mycobacterium ulcerans/Mycobacterium marinum (MuMC) may cause disease in both fish and humans. Here, we describe the emerg...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-08-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.00856-23 |
| _version_ | 1797741757109108736 |
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| author | Nadav Davidovich Andrei Makhon Gal Zizelski Valenci Zeev Dveyrin Tal Yahav Tobia Pretto Dan Tchernov Danny Morick |
| author_facet | Nadav Davidovich Andrei Makhon Gal Zizelski Valenci Zeev Dveyrin Tal Yahav Tobia Pretto Dan Tchernov Danny Morick |
| author_sort | Nadav Davidovich |
| collection | DOAJ |
| description | ABSTRACT Among the numerous pathogenic nontuberculous mycobacteria (NTM), which may cause disease in both poikilothermic and homoeothermic organisms, members of the unique clade Mycobacterium ulcerans/Mycobacterium marinum (MuMC) may cause disease in both fish and humans. Here, we describe the emergence of Mycobacterium pseudoshottsii, one of the four MuMC members, in Israel. For many years, M. marinum was the dominant NTM that was diagnosed in Israel as a fish pathogen. To the best of our knowledge, this is the first isolation and genomic characterization of M. pseudoshottsii infecting edible fish from two different fish species farmed in offshore sea cages in the eastern Mediterranean as well as in a recirculating aquaculture system in Israel. We compared the M. pseudoshottsii whole-genome sequences to all available genomic sequences of MuMC in free, publicly accessible databases. IMPORTANCE Mycobacterium pseudoshottsii was first detected in 1997 in the USA, infecting wild striped bass (Morone saxatilis). Since then, several reports from different countries worldwide have shown its capacity to become established in new regions as well as its pathogenicity to saltwater and euryhaline finfish of different genera. Our phylogenetic analysis revealed that the Mycobacterium ulcerans/Mycobacterium marinum clade (MuMC) is divided into two main branches: one that includes M. marinum and M. pseudoshottsii, and the second, which includes other M. marinum isolates as well as two isolates of M. shottsii. Our results reinforce the proposition that the geographical distribution of M. pseudoshottsii is much more extensive than is commonly believed. The emergence of M. pseudoshottsii in different parts of the world and its pathogenic traits that affect finfish of different genera may be a cause for concern among fish farmers, researchers, and environmental organizations. |
| first_indexed | 2024-03-12T14:31:15Z |
| format | Article |
| id | doaj.art-4fb9765e4e034041af42eac4a47d9040 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-12T14:31:15Z |
| publishDate | 2023-08-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-4fb9765e4e034041af42eac4a47d90402023-08-17T13:04:15ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972023-08-0111410.1128/spectrum.00856-23Identification of Mycobacterium pseudoshottsii in the Eastern MediterraneanNadav Davidovich0Andrei Makhon1Gal Zizelski Valenci2Zeev Dveyrin3Tal Yahav4Tobia Pretto5Dan Tchernov6Danny Morick7Morris Kahn Marine Research Station, University of Haifa, Haifa, IsraelNational Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelNational Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelNational Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelBioinformatics Services Unit, University of Haifa, Haifa, IsraelIstituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Padua, ItalyMorris Kahn Marine Research Station, University of Haifa, Haifa, IsraelMorris Kahn Marine Research Station, University of Haifa, Haifa, IsraelABSTRACT Among the numerous pathogenic nontuberculous mycobacteria (NTM), which may cause disease in both poikilothermic and homoeothermic organisms, members of the unique clade Mycobacterium ulcerans/Mycobacterium marinum (MuMC) may cause disease in both fish and humans. Here, we describe the emergence of Mycobacterium pseudoshottsii, one of the four MuMC members, in Israel. For many years, M. marinum was the dominant NTM that was diagnosed in Israel as a fish pathogen. To the best of our knowledge, this is the first isolation and genomic characterization of M. pseudoshottsii infecting edible fish from two different fish species farmed in offshore sea cages in the eastern Mediterranean as well as in a recirculating aquaculture system in Israel. We compared the M. pseudoshottsii whole-genome sequences to all available genomic sequences of MuMC in free, publicly accessible databases. IMPORTANCE Mycobacterium pseudoshottsii was first detected in 1997 in the USA, infecting wild striped bass (Morone saxatilis). Since then, several reports from different countries worldwide have shown its capacity to become established in new regions as well as its pathogenicity to saltwater and euryhaline finfish of different genera. Our phylogenetic analysis revealed that the Mycobacterium ulcerans/Mycobacterium marinum clade (MuMC) is divided into two main branches: one that includes M. marinum and M. pseudoshottsii, and the second, which includes other M. marinum isolates as well as two isolates of M. shottsii. Our results reinforce the proposition that the geographical distribution of M. pseudoshottsii is much more extensive than is commonly believed. The emergence of M. pseudoshottsii in different parts of the world and its pathogenic traits that affect finfish of different genera may be a cause for concern among fish farmers, researchers, and environmental organizations.https://journals.asm.org/doi/10.1128/spectrum.00856-23Mycobacterium pseudoshottsiinontuberculous mycobacteriaIsraelemerging pathogen |
| spellingShingle | Nadav Davidovich Andrei Makhon Gal Zizelski Valenci Zeev Dveyrin Tal Yahav Tobia Pretto Dan Tchernov Danny Morick Identification of Mycobacterium pseudoshottsii in the Eastern Mediterranean Microbiology Spectrum Mycobacterium pseudoshottsii nontuberculous mycobacteria Israel emerging pathogen |
| title | Identification of Mycobacterium pseudoshottsii in the Eastern Mediterranean |
| title_full | Identification of Mycobacterium pseudoshottsii in the Eastern Mediterranean |
| title_fullStr | Identification of Mycobacterium pseudoshottsii in the Eastern Mediterranean |
| title_full_unstemmed | Identification of Mycobacterium pseudoshottsii in the Eastern Mediterranean |
| title_short | Identification of Mycobacterium pseudoshottsii in the Eastern Mediterranean |
| title_sort | identification of mycobacterium pseudoshottsii in the eastern mediterranean |
| topic | Mycobacterium pseudoshottsii nontuberculous mycobacteria Israel emerging pathogen |
| url | https://journals.asm.org/doi/10.1128/spectrum.00856-23 |
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