B-A Chromosome Translocations Possessing an A Centromere Partly Overcome the Root-Restricted Process of Chromosome Elimination in Aegilops speltoides
Some eukaryotes exhibit dramatic genome size differences between cells of different organs, resulting from the programmed elimination of chromosomes. Aegilops speltoides is an annual diploid species from the Poaceae family, with a maximum number of eight B chromosomes (Bs) in addition to its inheren...
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.875523/full |
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| author | Daiyan Li Daiyan Li Daiyan Li Alevtina Ruban Alevtina Ruban Jörg Fuchs Houyang Kang Houyang Kang Andreas Houben |
| author_facet | Daiyan Li Daiyan Li Daiyan Li Alevtina Ruban Alevtina Ruban Jörg Fuchs Houyang Kang Houyang Kang Andreas Houben |
| author_sort | Daiyan Li |
| collection | DOAJ |
| description | Some eukaryotes exhibit dramatic genome size differences between cells of different organs, resulting from the programmed elimination of chromosomes. Aegilops speltoides is an annual diploid species from the Poaceae family, with a maximum number of eight B chromosomes (Bs) in addition to its inherent seven pairs of standard A chromosomes (As). The Bs of this species undergo precise elimination in roots early in embryo development. In areal parts of the plant, the number of Bs is stable. To affect the root restricted process of B chromosome elimination, we employed X-ray mutagenesis, and different types of restructured Bs were identified. Standard Bs were observed in all analyzed shoots of mutagenized plants, while B-A translocations were only observed in 35.7% of F1 plants. In total 40 different B variants inconsistently escaped the elimination process in roots. As a result, mosaicism of B chromosome variants was found in roots. Only a small B chromosome fragment fused to an A chromosome was stably maintained in roots and shoots across F1 to F3 generations. The absence of B-A translocation chromosomes possessing a derived B centromere in root cells implies that the centromere of the B is a key component of the chromosome elimination process. |
| first_indexed | 2024-12-22T21:38:22Z |
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| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-22T21:38:22Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
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| spelling | doaj.art-4fbac8d65ac6476badfcd63b85889c462022-12-21T18:11:41ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-03-011010.3389/fcell.2022.875523875523B-A Chromosome Translocations Possessing an A Centromere Partly Overcome the Root-Restricted Process of Chromosome Elimination in Aegilops speltoidesDaiyan Li0Daiyan Li1Daiyan Li2Alevtina Ruban3Alevtina Ruban4Jörg Fuchs5Houyang Kang6Houyang Kang7Andreas Houben8Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, GermanyState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaLeibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, GermanyKWS SAAT SE & Co. KGaA, Einbeck, GermanyLeibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, GermanyState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaLeibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, GermanySome eukaryotes exhibit dramatic genome size differences between cells of different organs, resulting from the programmed elimination of chromosomes. Aegilops speltoides is an annual diploid species from the Poaceae family, with a maximum number of eight B chromosomes (Bs) in addition to its inherent seven pairs of standard A chromosomes (As). The Bs of this species undergo precise elimination in roots early in embryo development. In areal parts of the plant, the number of Bs is stable. To affect the root restricted process of B chromosome elimination, we employed X-ray mutagenesis, and different types of restructured Bs were identified. Standard Bs were observed in all analyzed shoots of mutagenized plants, while B-A translocations were only observed in 35.7% of F1 plants. In total 40 different B variants inconsistently escaped the elimination process in roots. As a result, mosaicism of B chromosome variants was found in roots. Only a small B chromosome fragment fused to an A chromosome was stably maintained in roots and shoots across F1 to F3 generations. The absence of B-A translocation chromosomes possessing a derived B centromere in root cells implies that the centromere of the B is a key component of the chromosome elimination process.https://www.frontiersin.org/articles/10.3389/fcell.2022.875523/fullAegilops speltoidessupernumerary B chromosomeX-ray irradiationB-A translocation chromosomeprogrammed chromosome eliminationmicronuclei |
| spellingShingle | Daiyan Li Daiyan Li Daiyan Li Alevtina Ruban Alevtina Ruban Jörg Fuchs Houyang Kang Houyang Kang Andreas Houben B-A Chromosome Translocations Possessing an A Centromere Partly Overcome the Root-Restricted Process of Chromosome Elimination in Aegilops speltoides Frontiers in Cell and Developmental Biology Aegilops speltoides supernumerary B chromosome X-ray irradiation B-A translocation chromosome programmed chromosome elimination micronuclei |
| title | B-A Chromosome Translocations Possessing an A Centromere Partly Overcome the Root-Restricted Process of Chromosome Elimination in Aegilops speltoides |
| title_full | B-A Chromosome Translocations Possessing an A Centromere Partly Overcome the Root-Restricted Process of Chromosome Elimination in Aegilops speltoides |
| title_fullStr | B-A Chromosome Translocations Possessing an A Centromere Partly Overcome the Root-Restricted Process of Chromosome Elimination in Aegilops speltoides |
| title_full_unstemmed | B-A Chromosome Translocations Possessing an A Centromere Partly Overcome the Root-Restricted Process of Chromosome Elimination in Aegilops speltoides |
| title_short | B-A Chromosome Translocations Possessing an A Centromere Partly Overcome the Root-Restricted Process of Chromosome Elimination in Aegilops speltoides |
| title_sort | b a chromosome translocations possessing an a centromere partly overcome the root restricted process of chromosome elimination in aegilops speltoides |
| topic | Aegilops speltoides supernumerary B chromosome X-ray irradiation B-A translocation chromosome programmed chromosome elimination micronuclei |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.875523/full |
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