An <i>O</i>-Demethylation Metabolite of Rabeprazole Sulfide by <i>Cunninghamella blakesleeana</i> 3.970 Biotransformation
To explore the potential metabolites from rabeprazole sulfide, seven strains of filamentous fungi were screened for their biotransformation abilities. Among these strains, <i>Cunninghamella blakesleeana</i> 3.970 exhibited the best result. Four different culture media were screened in or...
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MDPI AG
2022-12-01
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| Series: | Catalysts |
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| Online Access: | https://www.mdpi.com/2073-4344/13/1/15 |
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| author | Ming Song Hongxiang Zhu Jian Wang Weizhuo Xu Wei Xu |
| author_facet | Ming Song Hongxiang Zhu Jian Wang Weizhuo Xu Wei Xu |
| author_sort | Ming Song |
| collection | DOAJ |
| description | To explore the potential metabolites from rabeprazole sulfide, seven strains of filamentous fungi were screened for their biotransformation abilities. Among these strains, <i>Cunninghamella blakesleeana</i> 3.970 exhibited the best result. Four different culture media were screened in order to identify the most optimal for subsequent research. Single factors such as the initial pH of culture media, culture time, inoculation volume, and media volume were individually investigated to provide the optimum biotransformation conditions. Then, an orthogonal optimization process using a five-factor, four-level L<sub>16</sub>(4<sup>5</sup>) experiment was designed and performed. Finally, when the substrate concentration is 3 g/L, one major metabolite was detected with a transformation rate of 72.4%. Isolated by semipreparative HPLC, this metabolite was further detected by ESI-MS and NMR. The final data analysis indicated that the metabolite is <i>O</i>-demethylation rabeprazole sulfide. |
| first_indexed | 2024-03-09T13:16:20Z |
| format | Article |
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| issn | 2073-4344 |
| language | English |
| last_indexed | 2024-03-09T13:16:20Z |
| publishDate | 2022-12-01 |
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| series | Catalysts |
| spelling | doaj.art-4fca1bbabb7742ec81238449b2f6f9de2023-11-30T21:35:51ZengMDPI AGCatalysts2073-43442022-12-011311510.3390/catal13010015An <i>O</i>-Demethylation Metabolite of Rabeprazole Sulfide by <i>Cunninghamella blakesleeana</i> 3.970 BiotransformationMing Song0Hongxiang Zhu1Jian Wang2Weizhuo Xu3Wei Xu4School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Life Sciences and Biopharmaceuticals, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Life Sciences and Biopharmaceuticals, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Life Sciences and Biopharmaceuticals, Shenyang Pharmaceutical University, Shenyang 110016, ChinaTo explore the potential metabolites from rabeprazole sulfide, seven strains of filamentous fungi were screened for their biotransformation abilities. Among these strains, <i>Cunninghamella blakesleeana</i> 3.970 exhibited the best result. Four different culture media were screened in order to identify the most optimal for subsequent research. Single factors such as the initial pH of culture media, culture time, inoculation volume, and media volume were individually investigated to provide the optimum biotransformation conditions. Then, an orthogonal optimization process using a five-factor, four-level L<sub>16</sub>(4<sup>5</sup>) experiment was designed and performed. Finally, when the substrate concentration is 3 g/L, one major metabolite was detected with a transformation rate of 72.4%. Isolated by semipreparative HPLC, this metabolite was further detected by ESI-MS and NMR. The final data analysis indicated that the metabolite is <i>O</i>-demethylation rabeprazole sulfide.https://www.mdpi.com/2073-4344/13/1/15rabeprazolesulfidebiotransformation<i>Cunninghamella blakesleeana</i> 3.970<i>O</i>-demethylation rabeprazole sulfide |
| spellingShingle | Ming Song Hongxiang Zhu Jian Wang Weizhuo Xu Wei Xu An <i>O</i>-Demethylation Metabolite of Rabeprazole Sulfide by <i>Cunninghamella blakesleeana</i> 3.970 Biotransformation Catalysts rabeprazole sulfide biotransformation <i>Cunninghamella blakesleeana</i> 3.970 <i>O</i>-demethylation rabeprazole sulfide |
| title | An <i>O</i>-Demethylation Metabolite of Rabeprazole Sulfide by <i>Cunninghamella blakesleeana</i> 3.970 Biotransformation |
| title_full | An <i>O</i>-Demethylation Metabolite of Rabeprazole Sulfide by <i>Cunninghamella blakesleeana</i> 3.970 Biotransformation |
| title_fullStr | An <i>O</i>-Demethylation Metabolite of Rabeprazole Sulfide by <i>Cunninghamella blakesleeana</i> 3.970 Biotransformation |
| title_full_unstemmed | An <i>O</i>-Demethylation Metabolite of Rabeprazole Sulfide by <i>Cunninghamella blakesleeana</i> 3.970 Biotransformation |
| title_short | An <i>O</i>-Demethylation Metabolite of Rabeprazole Sulfide by <i>Cunninghamella blakesleeana</i> 3.970 Biotransformation |
| title_sort | i o i demethylation metabolite of rabeprazole sulfide by i cunninghamella blakesleeana i 3 970 biotransformation |
| topic | rabeprazole sulfide biotransformation <i>Cunninghamella blakesleeana</i> 3.970 <i>O</i>-demethylation rabeprazole sulfide |
| url | https://www.mdpi.com/2073-4344/13/1/15 |
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