Genetic diversity of Ehrlichia canisstrains from naturally infected dogs in Rio de Janeiro, Brazil
The aim of this study was to characterize Ehrlichia canis strains from naturally infected dogs in Rio de Janeiro, Brazil. In addition, all the clinical and hematological findings observed in these dogs were reported. PCR targeting the 16S rRNA gene was used for diagnostic purposes, and the TRP19 and...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Colégio Brasileiro de Parasitologia Veterinaria
|
| Series: | Revista Brasileira de Parasitologia Veterinária |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612014000300301&lng=en&tlng=en |
| _version_ | 1818682806468870144 |
|---|---|
| author | Renata Fernandes Ferreira Aloysio de Mello Figueiredo Cerqueira Tatiana Xavier de Castro Eliane de Oliveira Ferreira Felipe Piedade Gonçalves Neves André Victor Barbosa Daniel de Barros Macieira Nádia Regina Pereira Almosny |
| author_facet | Renata Fernandes Ferreira Aloysio de Mello Figueiredo Cerqueira Tatiana Xavier de Castro Eliane de Oliveira Ferreira Felipe Piedade Gonçalves Neves André Victor Barbosa Daniel de Barros Macieira Nádia Regina Pereira Almosny |
| author_sort | Renata Fernandes Ferreira |
| collection | DOAJ |
| description | The aim of this study was to characterize Ehrlichia canis strains from naturally infected dogs in Rio de Janeiro, Brazil. In addition, all the clinical and hematological findings observed in these dogs were reported. PCR targeting the 16S rRNA gene was used for diagnostic purposes, and the TRP19 and TRP36 genes were sequenced to evaluate the genetic diversity. Fifteen samples were positive for E. canis. The polymerase chain reaction for the TRP19 gene resulted in 11 amplicons (11/15), which were cloned into the pGEM-T easy vector for sequencing. The complete sequence of TRP19 gene was compared to those in the GenBank, revealing high identicalness. Phylogenetic analysis on the TRP36 gene sequences demonstrated two distinct strains from two dogs, named 56C and 70C. The 56C strain was grouped with the strain Cuiaba 16, which is a hybrid strain formed by Brazilian and US genogroups; and the 70C strain was grouped with other strains of the US genogroup, thus suggesting that there are at least two genogroups of E. canis in Rio de Janeiro (US and Brazilian). Those animals, in which the 70C and 56C strains were isolated, showed distinct clinical and hematological manifestations of 1the disease. The appearance of different genotypes may express new phenotypes, thus resulting in different forms of presentation of the disease and making its diagnosis more complex. |
| first_indexed | 2024-12-17T10:24:42Z |
| format | Article |
| id | doaj.art-4fcace1239b34dfd9e593048cc0a5460 |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-12-17T10:24:42Z |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-4fcace1239b34dfd9e593048cc0a54602022-12-21T21:52:42ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-296123330130810.1590/S1984-29612014055S1984-29612014000300301Genetic diversity of Ehrlichia canisstrains from naturally infected dogs in Rio de Janeiro, BrazilRenata Fernandes FerreiraAloysio de Mello Figueiredo CerqueiraTatiana Xavier de CastroEliane de Oliveira FerreiraFelipe Piedade Gonçalves NevesAndré Victor BarbosaDaniel de Barros MacieiraNádia Regina Pereira AlmosnyThe aim of this study was to characterize Ehrlichia canis strains from naturally infected dogs in Rio de Janeiro, Brazil. In addition, all the clinical and hematological findings observed in these dogs were reported. PCR targeting the 16S rRNA gene was used for diagnostic purposes, and the TRP19 and TRP36 genes were sequenced to evaluate the genetic diversity. Fifteen samples were positive for E. canis. The polymerase chain reaction for the TRP19 gene resulted in 11 amplicons (11/15), which were cloned into the pGEM-T easy vector for sequencing. The complete sequence of TRP19 gene was compared to those in the GenBank, revealing high identicalness. Phylogenetic analysis on the TRP36 gene sequences demonstrated two distinct strains from two dogs, named 56C and 70C. The 56C strain was grouped with the strain Cuiaba 16, which is a hybrid strain formed by Brazilian and US genogroups; and the 70C strain was grouped with other strains of the US genogroup, thus suggesting that there are at least two genogroups of E. canis in Rio de Janeiro (US and Brazilian). Those animals, in which the 70C and 56C strains were isolated, showed distinct clinical and hematological manifestations of 1the disease. The appearance of different genotypes may express new phenotypes, thus resulting in different forms of presentation of the disease and making its diagnosis more complex.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612014000300301&lng=en&tlng=enDogsgenogroupsTRP19TRP36CME |
| spellingShingle | Renata Fernandes Ferreira Aloysio de Mello Figueiredo Cerqueira Tatiana Xavier de Castro Eliane de Oliveira Ferreira Felipe Piedade Gonçalves Neves André Victor Barbosa Daniel de Barros Macieira Nádia Regina Pereira Almosny Genetic diversity of Ehrlichia canisstrains from naturally infected dogs in Rio de Janeiro, Brazil Revista Brasileira de Parasitologia Veterinária Dogs genogroups TRP19 TRP36 CME |
| title | Genetic diversity of Ehrlichia canisstrains from naturally infected dogs in Rio de Janeiro, Brazil |
| title_full | Genetic diversity of Ehrlichia canisstrains from naturally infected dogs in Rio de Janeiro, Brazil |
| title_fullStr | Genetic diversity of Ehrlichia canisstrains from naturally infected dogs in Rio de Janeiro, Brazil |
| title_full_unstemmed | Genetic diversity of Ehrlichia canisstrains from naturally infected dogs in Rio de Janeiro, Brazil |
| title_short | Genetic diversity of Ehrlichia canisstrains from naturally infected dogs in Rio de Janeiro, Brazil |
| title_sort | genetic diversity of ehrlichia canisstrains from naturally infected dogs in rio de janeiro brazil |
| topic | Dogs genogroups TRP19 TRP36 CME |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612014000300301&lng=en&tlng=en |
| work_keys_str_mv | AT renatafernandesferreira geneticdiversityofehrlichiacanisstrainsfromnaturallyinfecteddogsinriodejaneirobrazil AT aloysiodemellofigueiredocerqueira geneticdiversityofehrlichiacanisstrainsfromnaturallyinfecteddogsinriodejaneirobrazil AT tatianaxavierdecastro geneticdiversityofehrlichiacanisstrainsfromnaturallyinfecteddogsinriodejaneirobrazil AT elianedeoliveiraferreira geneticdiversityofehrlichiacanisstrainsfromnaturallyinfecteddogsinriodejaneirobrazil AT felipepiedadegoncalvesneves geneticdiversityofehrlichiacanisstrainsfromnaturallyinfecteddogsinriodejaneirobrazil AT andrevictorbarbosa geneticdiversityofehrlichiacanisstrainsfromnaturallyinfecteddogsinriodejaneirobrazil AT danieldebarrosmacieira geneticdiversityofehrlichiacanisstrainsfromnaturallyinfecteddogsinriodejaneirobrazil AT nadiareginapereiraalmosny geneticdiversityofehrlichiacanisstrainsfromnaturallyinfecteddogsinriodejaneirobrazil |