Metabolomic Profiling Unveils the Impact of Non-Doped and Heteroatom-Doped Carbon Nanodots on Zebrafish (<i>Danio rerio</i>) Embryos
Recently, concern has been raised over the transport, transformation, and fate of carbon nanodots (CNDs) after their release into the environment. Their toxicity towards organisms and humans has recently been addressed as an important issue. In this study, a metabolomic approach was employed to obta...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/11/2/483 |
| _version_ | 1827589213668769792 |
|---|---|
| author | Theodoros G. Chatzimitakos Claire Pliatsika Ieremias Chousidis Ioannis D. Leonardos Constantine D. Stalikas |
| author_facet | Theodoros G. Chatzimitakos Claire Pliatsika Ieremias Chousidis Ioannis D. Leonardos Constantine D. Stalikas |
| author_sort | Theodoros G. Chatzimitakos |
| collection | DOAJ |
| description | Recently, concern has been raised over the transport, transformation, and fate of carbon nanodots (CNDs) after their release into the environment. Their toxicity towards organisms and humans has recently been addressed as an important issue. In this study, a metabolomic approach was employed to obtain an insight into the effect of CNDs (either pristine or doped with nitrogen and nitrogen/sulfur) on zebrafish. Embryos were exposed to concentrations corresponding to lethal concentration (LC) LC<sub>50</sub> (550, 400, and 150 μg mL<sup>−1</sup>), LC<sub>50/2</sub> (275, 200, and 75 μg mL<sup>−1</sup>), and LC<sub>50/4</sub> (138, 100, and 38 μg mL<sup>−1</sup>) of the three CNDs (non-doped, N-doped, and N,S-codoped, respectively) to scrutinize the interactions of the CNDs with the larvae. Numerous differences in the metabolic pathways were recorded in all cases. Seven metabolic pathways were detected in the control larvae. When the larvae were exposed to concentrations equal to LC<sub>50</sub>, LC<sub>50/2</sub>, and LC<sub>50/4</sub> of non-doped CNDs, 12, 12, and 3 metabolic pathways were detected, respectively. In the case of N-doped CNDs, 4, 7, and 4 pathways were detected, while in the case of N,S-codoped CNDs, 8, 5, and 5 pathways were detected when exposed to concentrations of LC<sub>50</sub>, LC<sub>50/2</sub>, and LC<sub>50/4</sub>, respectively. In all cases, certain metabolic pathways were altered while others were either down-regulated or up-regulated. Some of these changes include the activation of alanine, aspartate, and glutamate metabolism, aminoacyl-tRNA biosynthesis, butanoate metabolism, D-glutamine, and D-glutamate metabolism, glutathione metabolism, selenoamino acid metabolism, valine, leucine, and isoleucine degradation pathways. Moreover, the deactivation of starch and sucrose metabolism, the glycine, serine, and threonine metabolism, among others, were recorded. Our findings underline the importance to further study the impact of CNDs on marine organisms. As zebrafish has been shown to share many similarities with humans in bioprocesses and genome, it can be assumed that CNDs may also pose a threat to human health. |
| first_indexed | 2024-03-09T00:53:26Z |
| format | Article |
| id | doaj.art-4fcaf2b2428343c6bb7f070c0ce15bad |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T00:53:26Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-4fcaf2b2428343c6bb7f070c0ce15bad2023-12-11T17:03:57ZengMDPI AGNanomaterials2079-49912021-02-0111248310.3390/nano11020483Metabolomic Profiling Unveils the Impact of Non-Doped and Heteroatom-Doped Carbon Nanodots on Zebrafish (<i>Danio rerio</i>) EmbryosTheodoros G. Chatzimitakos0Claire Pliatsika1Ieremias Chousidis2Ioannis D. Leonardos3Constantine D. Stalikas4Laboratory of Analytical Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, GreeceLaboratory of Analytical Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, GreeceLaboratory of Zoology, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, GreeceLaboratory of Zoology, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, GreeceLaboratory of Analytical Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, GreeceRecently, concern has been raised over the transport, transformation, and fate of carbon nanodots (CNDs) after their release into the environment. Their toxicity towards organisms and humans has recently been addressed as an important issue. In this study, a metabolomic approach was employed to obtain an insight into the effect of CNDs (either pristine or doped with nitrogen and nitrogen/sulfur) on zebrafish. Embryos were exposed to concentrations corresponding to lethal concentration (LC) LC<sub>50</sub> (550, 400, and 150 μg mL<sup>−1</sup>), LC<sub>50/2</sub> (275, 200, and 75 μg mL<sup>−1</sup>), and LC<sub>50/4</sub> (138, 100, and 38 μg mL<sup>−1</sup>) of the three CNDs (non-doped, N-doped, and N,S-codoped, respectively) to scrutinize the interactions of the CNDs with the larvae. Numerous differences in the metabolic pathways were recorded in all cases. Seven metabolic pathways were detected in the control larvae. When the larvae were exposed to concentrations equal to LC<sub>50</sub>, LC<sub>50/2</sub>, and LC<sub>50/4</sub> of non-doped CNDs, 12, 12, and 3 metabolic pathways were detected, respectively. In the case of N-doped CNDs, 4, 7, and 4 pathways were detected, while in the case of N,S-codoped CNDs, 8, 5, and 5 pathways were detected when exposed to concentrations of LC<sub>50</sub>, LC<sub>50/2</sub>, and LC<sub>50/4</sub>, respectively. In all cases, certain metabolic pathways were altered while others were either down-regulated or up-regulated. Some of these changes include the activation of alanine, aspartate, and glutamate metabolism, aminoacyl-tRNA biosynthesis, butanoate metabolism, D-glutamine, and D-glutamate metabolism, glutathione metabolism, selenoamino acid metabolism, valine, leucine, and isoleucine degradation pathways. Moreover, the deactivation of starch and sucrose metabolism, the glycine, serine, and threonine metabolism, among others, were recorded. Our findings underline the importance to further study the impact of CNDs on marine organisms. As zebrafish has been shown to share many similarities with humans in bioprocesses and genome, it can be assumed that CNDs may also pose a threat to human health.https://www.mdpi.com/2079-4991/11/2/483zebrafishmetabolomicsnon-doped carbon nanodotsN-doped carbon nanodotsN,S-codoped carbon nanodots |
| spellingShingle | Theodoros G. Chatzimitakos Claire Pliatsika Ieremias Chousidis Ioannis D. Leonardos Constantine D. Stalikas Metabolomic Profiling Unveils the Impact of Non-Doped and Heteroatom-Doped Carbon Nanodots on Zebrafish (<i>Danio rerio</i>) Embryos Nanomaterials zebrafish metabolomics non-doped carbon nanodots N-doped carbon nanodots N,S-codoped carbon nanodots |
| title | Metabolomic Profiling Unveils the Impact of Non-Doped and Heteroatom-Doped Carbon Nanodots on Zebrafish (<i>Danio rerio</i>) Embryos |
| title_full | Metabolomic Profiling Unveils the Impact of Non-Doped and Heteroatom-Doped Carbon Nanodots on Zebrafish (<i>Danio rerio</i>) Embryos |
| title_fullStr | Metabolomic Profiling Unveils the Impact of Non-Doped and Heteroatom-Doped Carbon Nanodots on Zebrafish (<i>Danio rerio</i>) Embryos |
| title_full_unstemmed | Metabolomic Profiling Unveils the Impact of Non-Doped and Heteroatom-Doped Carbon Nanodots on Zebrafish (<i>Danio rerio</i>) Embryos |
| title_short | Metabolomic Profiling Unveils the Impact of Non-Doped and Heteroatom-Doped Carbon Nanodots on Zebrafish (<i>Danio rerio</i>) Embryos |
| title_sort | metabolomic profiling unveils the impact of non doped and heteroatom doped carbon nanodots on zebrafish i danio rerio i embryos |
| topic | zebrafish metabolomics non-doped carbon nanodots N-doped carbon nanodots N,S-codoped carbon nanodots |
| url | https://www.mdpi.com/2079-4991/11/2/483 |
| work_keys_str_mv | AT theodorosgchatzimitakos metabolomicprofilingunveilstheimpactofnondopedandheteroatomdopedcarbonnanodotsonzebrafishidaniorerioiembryos AT clairepliatsika metabolomicprofilingunveilstheimpactofnondopedandheteroatomdopedcarbonnanodotsonzebrafishidaniorerioiembryos AT ieremiaschousidis metabolomicprofilingunveilstheimpactofnondopedandheteroatomdopedcarbonnanodotsonzebrafishidaniorerioiembryos AT ioannisdleonardos metabolomicprofilingunveilstheimpactofnondopedandheteroatomdopedcarbonnanodotsonzebrafishidaniorerioiembryos AT constantinedstalikas metabolomicprofilingunveilstheimpactofnondopedandheteroatomdopedcarbonnanodotsonzebrafishidaniorerioiembryos |