Analgesic effect of dl-THP on inflammatory pain mediated by suppressing spinal TRPV1 and P2X3 receptors in rats
Tetrahydropalmatine (dl-THP) demonstrates an analgesic effect in animal models of neuropathic and inflammatory pain, however, the underlying mechanisms of its pharmacological action within the spinal cord remains unclear. Both P2X3 receptor and TRPV1 are associated with the development and progressi...
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| Format: | Article |
| Language: | English |
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IMR Press
2021-04-01
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| Series: | Frontiers in Bioscience-Landmark |
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| Online Access: | https://www.imrpress.com/journal/FBL/26/5/10.52586/4919 |
| _version_ | 1819052146535956480 |
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| author | Yan Wang Rui-Rui Wang Wei Sun Chao Lou Fan Yang Ting He Xiao-Liang Wang Fa-Le Cao Jun Chen |
| author_facet | Yan Wang Rui-Rui Wang Wei Sun Chao Lou Fan Yang Ting He Xiao-Liang Wang Fa-Le Cao Jun Chen |
| author_sort | Yan Wang |
| collection | DOAJ |
| description | Tetrahydropalmatine (dl-THP) demonstrates an analgesic effect in animal models of neuropathic and inflammatory pain, however, the underlying mechanisms of its pharmacological action within the spinal cord remains unclear. Both P2X3 receptor and TRPV1 are associated with the development and progression of such neuropathic and inflammatory pain. Here, we found that both pre-treatment and post-treatment with dl-THP could attenuate Bee Venom (BV)-induced persistent spontaneous pain-related behaviors in rats. Further, the dl-THP also exerted both preventive and therapeutic analgesic effects in BV-induced primary thermal and mechanical pain hypersensitivity as well as in mirror-image thermal pain hypersensitivity. The Rota-Rod treadmill test revealed that the dl-THP administration did not alter the rats’ motor coordinating performance. The TRPV1 and P2X3 receptor proteins increased markedly in the spinal cord of the rats following s.c. BV injection, which was significantly suppressed by dl-THP. These results suggest that dl-THP exerts a robust antihyperalgesia effect through down-regulation of P2X3 receptors and TRPV1 in inflammatory pain, providing a scientific basis for the translation of dl-THP treatment in clinics. |
| first_indexed | 2024-12-21T12:15:12Z |
| format | Article |
| id | doaj.art-4fcf16a2ae334626ba0bf18abfccb7f7 |
| institution | Directory Open Access Journal |
| issn | 2768-6701 |
| language | English |
| last_indexed | 2024-12-21T12:15:12Z |
| publishDate | 2021-04-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Frontiers in Bioscience-Landmark |
| spelling | doaj.art-4fcf16a2ae334626ba0bf18abfccb7f72022-12-21T19:04:27ZengIMR PressFrontiers in Bioscience-Landmark2768-67012021-04-0126511010.52586/4919s1093-9946(21)04605-1Analgesic effect of dl-THP on inflammatory pain mediated by suppressing spinal TRPV1 and P2X3 receptors in ratsYan Wang0Rui-Rui Wang1Wei Sun2Chao Lou3Fan Yang4Ting He5Xiao-Liang Wang6Fa-Le Cao7Jun Chen8Institute for Biomedical Sciences of Pain and Institute for Functional Brain Disorders, Tangdu Hospital, The Fourth Military Medical University, 710038 Xi’an, ChinaInstitute for Biomedical Sciences of Pain and Institute for Functional Brain Disorders, Tangdu Hospital, The Fourth Military Medical University, 710038 Xi’an, ChinaInstitute for Biomedical Sciences of Pain and Institute for Functional Brain Disorders, Tangdu Hospital, The Fourth Military Medical University, 710038 Xi’an, ChinaDepartment of Genetics, Northwest Women’s and Children’s Hospital, 710032 Xi’an, ChinaInstitute for Biomedical Sciences of Pain and Institute for Functional Brain Disorders, Tangdu Hospital, The Fourth Military Medical University, 710038 Xi’an, ChinaInstitute for Biomedical Sciences of Pain and Institute for Functional Brain Disorders, Tangdu Hospital, The Fourth Military Medical University, 710038 Xi’an, ChinaInstitute for Biomedical Sciences of Pain and Institute for Functional Brain Disorders, Tangdu Hospital, The Fourth Military Medical University, 710038 Xi’an, ChinaThe Department of Neurology, The 960th Hospital of PLA, 271000 Tai’an, ChinaInstitute for Biomedical Sciences of Pain and Institute for Functional Brain Disorders, Tangdu Hospital, The Fourth Military Medical University, 710038 Xi’an, ChinaTetrahydropalmatine (dl-THP) demonstrates an analgesic effect in animal models of neuropathic and inflammatory pain, however, the underlying mechanisms of its pharmacological action within the spinal cord remains unclear. Both P2X3 receptor and TRPV1 are associated with the development and progression of such neuropathic and inflammatory pain. Here, we found that both pre-treatment and post-treatment with dl-THP could attenuate Bee Venom (BV)-induced persistent spontaneous pain-related behaviors in rats. Further, the dl-THP also exerted both preventive and therapeutic analgesic effects in BV-induced primary thermal and mechanical pain hypersensitivity as well as in mirror-image thermal pain hypersensitivity. The Rota-Rod treadmill test revealed that the dl-THP administration did not alter the rats’ motor coordinating performance. The TRPV1 and P2X3 receptor proteins increased markedly in the spinal cord of the rats following s.c. BV injection, which was significantly suppressed by dl-THP. These results suggest that dl-THP exerts a robust antihyperalgesia effect through down-regulation of P2X3 receptors and TRPV1 in inflammatory pain, providing a scientific basis for the translation of dl-THP treatment in clinics.https://www.imrpress.com/journal/FBL/26/5/10.52586/4919inflammatory paindl-thptrpv1p2x3 receptor |
| spellingShingle | Yan Wang Rui-Rui Wang Wei Sun Chao Lou Fan Yang Ting He Xiao-Liang Wang Fa-Le Cao Jun Chen Analgesic effect of dl-THP on inflammatory pain mediated by suppressing spinal TRPV1 and P2X3 receptors in rats Frontiers in Bioscience-Landmark inflammatory pain dl-thp trpv1 p2x3 receptor |
| title | Analgesic effect of dl-THP on inflammatory pain mediated by suppressing spinal TRPV1 and P2X3 receptors in rats |
| title_full | Analgesic effect of dl-THP on inflammatory pain mediated by suppressing spinal TRPV1 and P2X3 receptors in rats |
| title_fullStr | Analgesic effect of dl-THP on inflammatory pain mediated by suppressing spinal TRPV1 and P2X3 receptors in rats |
| title_full_unstemmed | Analgesic effect of dl-THP on inflammatory pain mediated by suppressing spinal TRPV1 and P2X3 receptors in rats |
| title_short | Analgesic effect of dl-THP on inflammatory pain mediated by suppressing spinal TRPV1 and P2X3 receptors in rats |
| title_sort | analgesic effect of dl thp on inflammatory pain mediated by suppressing spinal trpv1 and p2x3 receptors in rats |
| topic | inflammatory pain dl-thp trpv1 p2x3 receptor |
| url | https://www.imrpress.com/journal/FBL/26/5/10.52586/4919 |
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