Co-Expression of Nogo-A in Dopaminergic Neurons of the Human Substantia Nigra Pars Compacta Is Reduced in Parkinson’s Disease
Parkinson’s disease is mainly characterized by a progressive loss of dopaminergic neurons in the substantia nigra pars compacta. Together with the small number, the high vulnerability of the dopaminergic neurons is a major pathogenic culprit of Parkinson’s disease. Our previous findings of a higher...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/12/3368 |
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| author | Gian-Carlo Eyer Stefano Di Santo Ekkehard Hewer Lukas Andereggen Stefanie Seiler Hans Rudolf Widmer |
| author_facet | Gian-Carlo Eyer Stefano Di Santo Ekkehard Hewer Lukas Andereggen Stefanie Seiler Hans Rudolf Widmer |
| author_sort | Gian-Carlo Eyer |
| collection | DOAJ |
| description | Parkinson’s disease is mainly characterized by a progressive loss of dopaminergic neurons in the substantia nigra pars compacta. Together with the small number, the high vulnerability of the dopaminergic neurons is a major pathogenic culprit of Parkinson’s disease. Our previous findings of a higher survival of dopaminergic neurons in the substantia nigra co-expressing Nogo-A in an animal model of Parkinson’s disease suggested that Nogo-A may be associated with dopaminergic neurons resilience against Parkinson’s disease neurodegeneration. In the present study, we have addressed the expression of Nogo-A in the dopaminergic neurons in the substantia nigra in postmortem specimens of diseased and non-diseased subjects of different ages. For this purpose, in a collaborative effort we developed a tissue micro array (TMA) that allows for simultaneous staining of many samples in a single run. Interestingly, and in contrast to the observations gathered during normal aging and in the animal model of Parkinson’s disease, increasing age was significantly associated with a lower co-expression of Nogo-A in nigral dopaminergic neurons of patients with Parkinson’s disease. In sum, while Nogo-A expression in dopaminergic neurons is higher with increasing age, the opposite is the case in Parkinson’s disease. These observations suggest that Nogo-A might play a substantial role in the vulnerability of dopaminergic neurons in Parkinson’s disease. |
| first_indexed | 2024-03-10T04:25:41Z |
| format | Article |
| id | doaj.art-4fd009ac15814bfba75119bc20a917b9 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T04:25:41Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-4fd009ac15814bfba75119bc20a917b92023-11-23T07:36:49ZengMDPI AGCells2073-44092021-11-011012336810.3390/cells10123368Co-Expression of Nogo-A in Dopaminergic Neurons of the Human Substantia Nigra Pars Compacta Is Reduced in Parkinson’s DiseaseGian-Carlo Eyer0Stefano Di Santo1Ekkehard Hewer2Lukas Andereggen3Stefanie Seiler4Hans Rudolf Widmer5Neurocenter and Program for Regenerative Neuroscience, Department of Neurosurgery, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, SwitzerlandNeurocenter and Program for Regenerative Neuroscience, Department of Neurosurgery, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, SwitzerlandInstitute of Pathology, University of Bern, 3010 Bern, SwitzerlandNeurocenter and Program for Regenerative Neuroscience, Department of Neurosurgery, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, SwitzerlandNeurocenter and Program for Regenerative Neuroscience, Department of Neurosurgery, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, SwitzerlandNeurocenter and Program for Regenerative Neuroscience, Department of Neurosurgery, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, SwitzerlandParkinson’s disease is mainly characterized by a progressive loss of dopaminergic neurons in the substantia nigra pars compacta. Together with the small number, the high vulnerability of the dopaminergic neurons is a major pathogenic culprit of Parkinson’s disease. Our previous findings of a higher survival of dopaminergic neurons in the substantia nigra co-expressing Nogo-A in an animal model of Parkinson’s disease suggested that Nogo-A may be associated with dopaminergic neurons resilience against Parkinson’s disease neurodegeneration. In the present study, we have addressed the expression of Nogo-A in the dopaminergic neurons in the substantia nigra in postmortem specimens of diseased and non-diseased subjects of different ages. For this purpose, in a collaborative effort we developed a tissue micro array (TMA) that allows for simultaneous staining of many samples in a single run. Interestingly, and in contrast to the observations gathered during normal aging and in the animal model of Parkinson’s disease, increasing age was significantly associated with a lower co-expression of Nogo-A in nigral dopaminergic neurons of patients with Parkinson’s disease. In sum, while Nogo-A expression in dopaminergic neurons is higher with increasing age, the opposite is the case in Parkinson’s disease. These observations suggest that Nogo-A might play a substantial role in the vulnerability of dopaminergic neurons in Parkinson’s disease.https://www.mdpi.com/2073-4409/10/12/3368Nogo-AParkinson’s diseasetyrosine hydroxylasesubstantia nigra pars compactahumanimmunofluorescence |
| spellingShingle | Gian-Carlo Eyer Stefano Di Santo Ekkehard Hewer Lukas Andereggen Stefanie Seiler Hans Rudolf Widmer Co-Expression of Nogo-A in Dopaminergic Neurons of the Human Substantia Nigra Pars Compacta Is Reduced in Parkinson’s Disease Cells Nogo-A Parkinson’s disease tyrosine hydroxylase substantia nigra pars compacta human immunofluorescence |
| title | Co-Expression of Nogo-A in Dopaminergic Neurons of the Human Substantia Nigra Pars Compacta Is Reduced in Parkinson’s Disease |
| title_full | Co-Expression of Nogo-A in Dopaminergic Neurons of the Human Substantia Nigra Pars Compacta Is Reduced in Parkinson’s Disease |
| title_fullStr | Co-Expression of Nogo-A in Dopaminergic Neurons of the Human Substantia Nigra Pars Compacta Is Reduced in Parkinson’s Disease |
| title_full_unstemmed | Co-Expression of Nogo-A in Dopaminergic Neurons of the Human Substantia Nigra Pars Compacta Is Reduced in Parkinson’s Disease |
| title_short | Co-Expression of Nogo-A in Dopaminergic Neurons of the Human Substantia Nigra Pars Compacta Is Reduced in Parkinson’s Disease |
| title_sort | co expression of nogo a in dopaminergic neurons of the human substantia nigra pars compacta is reduced in parkinson s disease |
| topic | Nogo-A Parkinson’s disease tyrosine hydroxylase substantia nigra pars compacta human immunofluorescence |
| url | https://www.mdpi.com/2073-4409/10/12/3368 |
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