Activity regulates a cell type-specific mitochondrial phenotype in zebrafish lateral line hair cells
Hair cells of the inner ear are particularly sensitive to changes in mitochondria, the subcellular organelles necessary for energy production in all eukaryotic cells. There are over 30 mitochondrial deafness genes, and mitochondria are implicated in hair cell death following noise exposure, aminogly...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2023-03-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/80468 |
| _version_ | 1827227236668801024 |
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| author | Andrea McQuate Sharmon Knecht David W Raible |
| author_facet | Andrea McQuate Sharmon Knecht David W Raible |
| author_sort | Andrea McQuate |
| collection | DOAJ |
| description | Hair cells of the inner ear are particularly sensitive to changes in mitochondria, the subcellular organelles necessary for energy production in all eukaryotic cells. There are over 30 mitochondrial deafness genes, and mitochondria are implicated in hair cell death following noise exposure, aminoglycoside antibiotic exposure, as well as in age-related hearing loss. However, little is known about the basic aspects of hair cell mitochondrial biology. Using hair cells from the zebrafish lateral line as a model and serial block-face scanning electron microscopy, we have quantifiably characterized a unique hair cell mitochondrial phenotype that includes (1) a high mitochondrial volume and (2) specific mitochondrial architecture: multiple small mitochondria apically, and a reticular mitochondrial network basally. This phenotype develops gradually over the lifetime of the hair cell. Disrupting this mitochondrial phenotype with a mutation in opa1 impacts mitochondrial health and function. While hair cell activity is not required for the high mitochondrial volume, it shapes the mitochondrial architecture, with mechanotransduction necessary for all patterning, and synaptic transmission necessary for the development of mitochondrial networks. These results demonstrate the high degree to which hair cells regulate their mitochondria for optimal physiology and provide new insights into mitochondrial deafness. |
| first_indexed | 2024-03-12T14:57:26Z |
| format | Article |
| id | doaj.art-4fd143e6bd184f7fa9c8965550b48f90 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2025-03-21T17:54:45Z |
| publishDate | 2023-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-4fd143e6bd184f7fa9c8965550b48f902024-06-11T08:18:08ZengeLife Sciences Publications LtdeLife2050-084X2023-03-011210.7554/eLife.80468Activity regulates a cell type-specific mitochondrial phenotype in zebrafish lateral line hair cellsAndrea McQuate0https://orcid.org/0000-0002-2052-2004Sharmon Knecht1David W Raible2https://orcid.org/0000-0002-5342-5841Department of Biological Structure, University of Washington, Seattle, United States; Department of Otolaryngology-HNS, University of Washington, Seattle, United StatesDepartment of Biological Structure, University of Washington, Seattle, United StatesDepartment of Biological Structure, University of Washington, Seattle, United States; Department of Otolaryngology-HNS, University of Washington, Seattle, United StatesHair cells of the inner ear are particularly sensitive to changes in mitochondria, the subcellular organelles necessary for energy production in all eukaryotic cells. There are over 30 mitochondrial deafness genes, and mitochondria are implicated in hair cell death following noise exposure, aminoglycoside antibiotic exposure, as well as in age-related hearing loss. However, little is known about the basic aspects of hair cell mitochondrial biology. Using hair cells from the zebrafish lateral line as a model and serial block-face scanning electron microscopy, we have quantifiably characterized a unique hair cell mitochondrial phenotype that includes (1) a high mitochondrial volume and (2) specific mitochondrial architecture: multiple small mitochondria apically, and a reticular mitochondrial network basally. This phenotype develops gradually over the lifetime of the hair cell. Disrupting this mitochondrial phenotype with a mutation in opa1 impacts mitochondrial health and function. While hair cell activity is not required for the high mitochondrial volume, it shapes the mitochondrial architecture, with mechanotransduction necessary for all patterning, and synaptic transmission necessary for the development of mitochondrial networks. These results demonstrate the high degree to which hair cells regulate their mitochondria for optimal physiology and provide new insights into mitochondrial deafness.https://elifesciences.org/articles/80468hair cellsmitochondriaribbons |
| spellingShingle | Andrea McQuate Sharmon Knecht David W Raible Activity regulates a cell type-specific mitochondrial phenotype in zebrafish lateral line hair cells eLife hair cells mitochondria ribbons |
| title | Activity regulates a cell type-specific mitochondrial phenotype in zebrafish lateral line hair cells |
| title_full | Activity regulates a cell type-specific mitochondrial phenotype in zebrafish lateral line hair cells |
| title_fullStr | Activity regulates a cell type-specific mitochondrial phenotype in zebrafish lateral line hair cells |
| title_full_unstemmed | Activity regulates a cell type-specific mitochondrial phenotype in zebrafish lateral line hair cells |
| title_short | Activity regulates a cell type-specific mitochondrial phenotype in zebrafish lateral line hair cells |
| title_sort | activity regulates a cell type specific mitochondrial phenotype in zebrafish lateral line hair cells |
| topic | hair cells mitochondria ribbons |
| url | https://elifesciences.org/articles/80468 |
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