Molecular Identification of Lactic Acid Bacteria Producing Antimicrobial Agents from Bakasang, An Indonesian Traditional Fermented Fish Product

Abstract<br />Twenty seven strains of lactic acid bacteria (LAB) were isolated from bakasang, Indonesian traditional fermented fish product. In general, LAB have inhibitory activity againts pathogenic bacteria and spoilage bacteria. Screening for antimicrobia activity of isolates were performe...

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Main Authors: Helen Joan Lawalata, Langkah Sembiring, Endang Sutriswati Rahayu
Format: Article
Language:English
Published: Universitas Gadjah Mada, Yogyakarta 2015-11-01
Series:Indonesian Journal of Biotechnology
Online Access:http://journal.ugm.ac.id/ijbiotech/article/view/7839
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author Helen Joan Lawalata
Langkah Sembiring
Endang Sutriswati Rahayu
author_facet Helen Joan Lawalata
Langkah Sembiring
Endang Sutriswati Rahayu
author_sort Helen Joan Lawalata
collection DOAJ
description Abstract<br />Twenty seven strains of lactic acid bacteria (LAB) were isolated from bakasang, Indonesian traditional fermented fish product. In general, LAB have inhibitory activity againts pathogenic bacteria and spoilage bacteria. Screening for antimicrobia activity of isolates were performed with well-diffusion method. One isolate that was designed as Pediococcus BksC24 was the strongest against bacteria pathogenic and spoilage bacteria. This strain was further identified by 16S rRNA gen sequence comparison. Isolates LAB producing antimicrobial agents from bakasang were identified as Pediococcus acidilactici.<br />Keywords : Bakasang, LAB, antimicrobial, phenotypic characteristics, 16S rRNA gene
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spelling doaj.art-4fdc2da2584b4ef89a938112aa18f6912022-12-21T18:45:06ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412015-11-011626555Molecular Identification of Lactic Acid Bacteria Producing Antimicrobial Agents from Bakasang, An Indonesian Traditional Fermented Fish ProductHelen Joan LawalataLangkah SembiringEndang Sutriswati RahayuAbstract<br />Twenty seven strains of lactic acid bacteria (LAB) were isolated from bakasang, Indonesian traditional fermented fish product. In general, LAB have inhibitory activity againts pathogenic bacteria and spoilage bacteria. Screening for antimicrobia activity of isolates were performed with well-diffusion method. One isolate that was designed as Pediococcus BksC24 was the strongest against bacteria pathogenic and spoilage bacteria. This strain was further identified by 16S rRNA gen sequence comparison. Isolates LAB producing antimicrobial agents from bakasang were identified as Pediococcus acidilactici.<br />Keywords : Bakasang, LAB, antimicrobial, phenotypic characteristics, 16S rRNA genehttp://journal.ugm.ac.id/ijbiotech/article/view/7839
spellingShingle Helen Joan Lawalata
Langkah Sembiring
Endang Sutriswati Rahayu
Molecular Identification of Lactic Acid Bacteria Producing Antimicrobial Agents from Bakasang, An Indonesian Traditional Fermented Fish Product
Indonesian Journal of Biotechnology
title Molecular Identification of Lactic Acid Bacteria Producing Antimicrobial Agents from Bakasang, An Indonesian Traditional Fermented Fish Product
title_full Molecular Identification of Lactic Acid Bacteria Producing Antimicrobial Agents from Bakasang, An Indonesian Traditional Fermented Fish Product
title_fullStr Molecular Identification of Lactic Acid Bacteria Producing Antimicrobial Agents from Bakasang, An Indonesian Traditional Fermented Fish Product
title_full_unstemmed Molecular Identification of Lactic Acid Bacteria Producing Antimicrobial Agents from Bakasang, An Indonesian Traditional Fermented Fish Product
title_short Molecular Identification of Lactic Acid Bacteria Producing Antimicrobial Agents from Bakasang, An Indonesian Traditional Fermented Fish Product
title_sort molecular identification of lactic acid bacteria producing antimicrobial agents from bakasang an indonesian traditional fermented fish product
url http://journal.ugm.ac.id/ijbiotech/article/view/7839
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AT langkahsembiring molecularidentificationoflacticacidbacteriaproducingantimicrobialagentsfrombakasanganindonesiantraditionalfermentedfishproduct
AT endangsutriswatirahayu molecularidentificationoflacticacidbacteriaproducingantimicrobialagentsfrombakasanganindonesiantraditionalfermentedfishproduct