Physical and chemical mechanisms of tissue optical clearing
Summary: Advanced optical methods combined with various probes pave the way toward molecular imaging within living cells. However, major challenges are associated with the need to enhance the imaging resolution even further to the subcellular level for the imaging of larger tissues, as well as for i...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221001462 |
| _version_ | 1831586260403093504 |
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| author | Tingting Yu Jingtan Zhu Dongyu Li Dan Zhu |
| author_facet | Tingting Yu Jingtan Zhu Dongyu Li Dan Zhu |
| author_sort | Tingting Yu |
| collection | DOAJ |
| description | Summary: Advanced optical methods combined with various probes pave the way toward molecular imaging within living cells. However, major challenges are associated with the need to enhance the imaging resolution even further to the subcellular level for the imaging of larger tissues, as well as for in vivo studies. High scattering and absorption of opaque tissues limit the penetration of light into deep tissues and thus the optical imaging depth. Tissue optical clearing technique provides an innovative way to perform deep-tissue imaging. Recently, various optical clearing methods have been developed, which provide tissue clearing based on similar physical principles via different chemical approaches. Here, we introduce the mechanisms of the current clearing methods from fundamental physical and chemical perspectives, including the main physical principle, refractive index matching via various chemical approaches, such as dissociation of collagen, delipidation, decalcification, dehydration, and hyperhydration, to reduce scattering, as well as decolorization to reduce absorption. |
| first_indexed | 2024-12-17T21:33:22Z |
| format | Article |
| id | doaj.art-4fdc65a6559a45a3988965e26f5949ed |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-17T21:33:22Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-4fdc65a6559a45a3988965e26f5949ed2022-12-21T21:31:48ZengElsevieriScience2589-00422021-03-01243102178Physical and chemical mechanisms of tissue optical clearingTingting Yu0Jingtan Zhu1Dongyu Li2Dan Zhu3Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China; MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei 430074, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China; MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei 430074, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China; MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei 430074, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China; MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China; Corresponding authorSummary: Advanced optical methods combined with various probes pave the way toward molecular imaging within living cells. However, major challenges are associated with the need to enhance the imaging resolution even further to the subcellular level for the imaging of larger tissues, as well as for in vivo studies. High scattering and absorption of opaque tissues limit the penetration of light into deep tissues and thus the optical imaging depth. Tissue optical clearing technique provides an innovative way to perform deep-tissue imaging. Recently, various optical clearing methods have been developed, which provide tissue clearing based on similar physical principles via different chemical approaches. Here, we introduce the mechanisms of the current clearing methods from fundamental physical and chemical perspectives, including the main physical principle, refractive index matching via various chemical approaches, such as dissociation of collagen, delipidation, decalcification, dehydration, and hyperhydration, to reduce scattering, as well as decolorization to reduce absorption.http://www.sciencedirect.com/science/article/pii/S2589004221001462Optical ImagingBiological SciencesResearch MethodologiesBiological Sciences Tools |
| spellingShingle | Tingting Yu Jingtan Zhu Dongyu Li Dan Zhu Physical and chemical mechanisms of tissue optical clearing iScience Optical Imaging Biological Sciences Research Methodologies Biological Sciences Tools |
| title | Physical and chemical mechanisms of tissue optical clearing |
| title_full | Physical and chemical mechanisms of tissue optical clearing |
| title_fullStr | Physical and chemical mechanisms of tissue optical clearing |
| title_full_unstemmed | Physical and chemical mechanisms of tissue optical clearing |
| title_short | Physical and chemical mechanisms of tissue optical clearing |
| title_sort | physical and chemical mechanisms of tissue optical clearing |
| topic | Optical Imaging Biological Sciences Research Methodologies Biological Sciences Tools |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221001462 |
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