Resistance of Lactobacillus Sp. SKG 34 in The Digestive Tract of Rats Detected Using Random Amplified Polymorphic DNA Technique
In recent years, interest in probiotics, particularly Lactobacillus strains like Lactobacillus sp. SKG34, isolated from Sumbawa mare milk, has surged due to their potential health benefits. This strain holds promise as a probiotic agent, but understanding its behavior in the gastrointestinal (GI) tr...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2024-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2024/17/bioconf_ic-ftn2024_02004.pdf |
| _version_ | 1797235248480649216 |
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| author | Nocianitri Komang Ayu Pratiwi I Desak Putu Kartika Sujaya I Nengah |
| author_facet | Nocianitri Komang Ayu Pratiwi I Desak Putu Kartika Sujaya I Nengah |
| author_sort | Nocianitri Komang Ayu |
| collection | DOAJ |
| description | In recent years, interest in probiotics, particularly Lactobacillus strains like Lactobacillus sp. SKG34, isolated from Sumbawa mare milk, has surged due to their potential health benefits. This strain holds promise as a probiotic agent, but understanding its behavior in the gastrointestinal (GI) tract is essential for effective application. This study aimed to investigate the resistance and persistence of Lactobacillus sp. SKG34 in the GI tract of rats. Rats were orally administered the strain at a concentration of 10^8 cells for 30 days. The probiotic’s ability to modulate gut microbiota and improve host health is a key area of interest. By utilizing techniques like PCR-RAPD, this research sought to elucidate the fate of Lactobacillus sp. SKG34 in the complex environment of the rat GI tract. Results revealed a significant increase in LAB population in cecal contents, from 3.06 x 10^9 cfu/g in the control group to 1.25 x 10^10 cfu/g in the probiotic group. RAPD analysis also detected Lactobacillus sp. SKG34 in approximately 23% of the total population of lactic acid bacteria in cecal contents, indicating its resistance and proliferation within the GI tract. Further investigation is warranted to fully understand the potential of Lactobacillus sp. SKG34 as a beneficial probiotic strain for human application. |
| first_indexed | 2024-04-24T16:44:56Z |
| format | Article |
| id | doaj.art-4fded9a47e4244ca982de09d784a7955 |
| institution | Directory Open Access Journal |
| issn | 2117-4458 |
| language | English |
| last_indexed | 2024-04-24T16:44:56Z |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj.art-4fded9a47e4244ca982de09d784a79552024-03-29T08:28:18ZengEDP SciencesBIO Web of Conferences2117-44582024-01-01980200410.1051/bioconf/20249802004bioconf_ic-ftn2024_02004Resistance of Lactobacillus Sp. SKG 34 in The Digestive Tract of Rats Detected Using Random Amplified Polymorphic DNA TechniqueNocianitri Komang Ayu0Pratiwi I Desak Putu Kartika1Sujaya I Nengah2Food Technology Study Program, Faculty of Agricultural Technology, Udayana UniversityFood Technology Study Program, Faculty of Agricultural Technology, Udayana UniversityPublic Health Study Program, Faculty of Medicine, Udayana UniversityIn recent years, interest in probiotics, particularly Lactobacillus strains like Lactobacillus sp. SKG34, isolated from Sumbawa mare milk, has surged due to their potential health benefits. This strain holds promise as a probiotic agent, but understanding its behavior in the gastrointestinal (GI) tract is essential for effective application. This study aimed to investigate the resistance and persistence of Lactobacillus sp. SKG34 in the GI tract of rats. Rats were orally administered the strain at a concentration of 10^8 cells for 30 days. The probiotic’s ability to modulate gut microbiota and improve host health is a key area of interest. By utilizing techniques like PCR-RAPD, this research sought to elucidate the fate of Lactobacillus sp. SKG34 in the complex environment of the rat GI tract. Results revealed a significant increase in LAB population in cecal contents, from 3.06 x 10^9 cfu/g in the control group to 1.25 x 10^10 cfu/g in the probiotic group. RAPD analysis also detected Lactobacillus sp. SKG34 in approximately 23% of the total population of lactic acid bacteria in cecal contents, indicating its resistance and proliferation within the GI tract. Further investigation is warranted to fully understand the potential of Lactobacillus sp. SKG34 as a beneficial probiotic strain for human application.https://www.bio-conferences.org/articles/bioconf/pdf/2024/17/bioconf_ic-ftn2024_02004.pdf |
| spellingShingle | Nocianitri Komang Ayu Pratiwi I Desak Putu Kartika Sujaya I Nengah Resistance of Lactobacillus Sp. SKG 34 in The Digestive Tract of Rats Detected Using Random Amplified Polymorphic DNA Technique BIO Web of Conferences |
| title | Resistance of Lactobacillus Sp. SKG 34 in The Digestive Tract of Rats Detected Using Random Amplified Polymorphic DNA Technique |
| title_full | Resistance of Lactobacillus Sp. SKG 34 in The Digestive Tract of Rats Detected Using Random Amplified Polymorphic DNA Technique |
| title_fullStr | Resistance of Lactobacillus Sp. SKG 34 in The Digestive Tract of Rats Detected Using Random Amplified Polymorphic DNA Technique |
| title_full_unstemmed | Resistance of Lactobacillus Sp. SKG 34 in The Digestive Tract of Rats Detected Using Random Amplified Polymorphic DNA Technique |
| title_short | Resistance of Lactobacillus Sp. SKG 34 in The Digestive Tract of Rats Detected Using Random Amplified Polymorphic DNA Technique |
| title_sort | resistance of lactobacillus sp skg 34 in the digestive tract of rats detected using random amplified polymorphic dna technique |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2024/17/bioconf_ic-ftn2024_02004.pdf |
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