Evaluation of an in-house enzyme linked immuonosorbent assay (ELISA) for detection of anti – Fasciola gigantica antibodies in sheep
Tropical fasciolosis caused by infection with Fasciola gigantica is regarded as one of the most important single helminthes infections of ruminants in Asia and Africa. Fasciolosis diagnosis, due to low sensitivity of coprological diagnostic method has been challenging for a long period. To evaluate...
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| Format: | Article |
| Language: | English |
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Ferdowsi University of Mashhad
2012-10-01
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| Series: | The Iranian Journal of Veterinary Science and Technology |
| Subjects: | |
| Online Access: | https://ijvst.um.ac.ir/article_26571_a3e05789909c4162baf685dd5e0e1c3f.pdf |
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| author | Mohammad Hossein Razi Jalali Hossein Hamidinejat Massoud Ghorbanpoor Saleh Esmaeelzadeh Abbas Sohbati |
| author_facet | Mohammad Hossein Razi Jalali Hossein Hamidinejat Massoud Ghorbanpoor Saleh Esmaeelzadeh Abbas Sohbati |
| author_sort | Mohammad Hossein Razi Jalali |
| collection | DOAJ |
| description | Tropical fasciolosis caused by infection with Fasciola gigantica is regarded as one of the most important single helminthes infections of ruminants in Asia and Africa. Fasciolosis diagnosis, due to low sensitivity of coprological diagnostic method has been challenging for a long period. To evaluate the usefulness of ELISA for diagnosis of anti – Fasciola gigantica antibodies in parasitized sheep, crude, as well as excretory-secretory (E/S) antigens were examined with 50 positive and 45 negative serum samples. Results were compared with liver inspection in slaughterhouse. The cut off values were determined in 0.469 and 0.516 for crude and E/S antigen respectively. We found 82% of sensitivity, 95.6 of specificity, 95.3% positive predictive value and 83 % negative predictive value using crude antigen. We have also found 92% of sensitivity, 93% specificity, 93.8% positive predictive value and 91.4 % negative predictive value using E/S antigen. We concluded that ELISA, specially using E/S antigen, is a good tool for serodiagnosis of Fasciola gigantica infection in sheep herds. |
| first_indexed | 2024-12-10T13:38:31Z |
| format | Article |
| id | doaj.art-4fdf233d607d4c4f8f4d0497ff7250e7 |
| institution | Directory Open Access Journal |
| issn | 2008-465X 2423-6306 |
| language | English |
| last_indexed | 2024-12-10T13:38:31Z |
| publishDate | 2012-10-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | The Iranian Journal of Veterinary Science and Technology |
| spelling | doaj.art-4fdf233d607d4c4f8f4d0497ff7250e72022-12-22T01:46:46ZengFerdowsi University of MashhadThe Iranian Journal of Veterinary Science and Technology2008-465X2423-63062012-10-0142172410.22067/veterinary.v4i2.1616926571Evaluation of an in-house enzyme linked immuonosorbent assay (ELISA) for detection of anti – Fasciola gigantica antibodies in sheepMohammad Hossein Razi Jalali0Hossein Hamidinejat1Massoud Ghorbanpoor2Saleh Esmaeelzadeh3Abbas Sohbati4Departments of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, AhvazDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, AhvazDepartments of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, AhvazDepartments of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, AhvazGraduated of parasitology, Faculty of veterinary medicine , Shahid Chamran u nivercity , AhvazTropical fasciolosis caused by infection with Fasciola gigantica is regarded as one of the most important single helminthes infections of ruminants in Asia and Africa. Fasciolosis diagnosis, due to low sensitivity of coprological diagnostic method has been challenging for a long period. To evaluate the usefulness of ELISA for diagnosis of anti – Fasciola gigantica antibodies in parasitized sheep, crude, as well as excretory-secretory (E/S) antigens were examined with 50 positive and 45 negative serum samples. Results were compared with liver inspection in slaughterhouse. The cut off values were determined in 0.469 and 0.516 for crude and E/S antigen respectively. We found 82% of sensitivity, 95.6 of specificity, 95.3% positive predictive value and 83 % negative predictive value using crude antigen. We have also found 92% of sensitivity, 93% specificity, 93.8% positive predictive value and 91.4 % negative predictive value using E/S antigen. We concluded that ELISA, specially using E/S antigen, is a good tool for serodiagnosis of Fasciola gigantica infection in sheep herds.https://ijvst.um.ac.ir/article_26571_a3e05789909c4162baf685dd5e0e1c3f.pdffasciola giganticelisasheep |
| spellingShingle | Mohammad Hossein Razi Jalali Hossein Hamidinejat Massoud Ghorbanpoor Saleh Esmaeelzadeh Abbas Sohbati Evaluation of an in-house enzyme linked immuonosorbent assay (ELISA) for detection of anti – Fasciola gigantica antibodies in sheep The Iranian Journal of Veterinary Science and Technology fasciola gigantic elisa sheep |
| title | Evaluation of an in-house enzyme linked immuonosorbent assay (ELISA) for detection of anti – Fasciola gigantica antibodies in sheep |
| title_full | Evaluation of an in-house enzyme linked immuonosorbent assay (ELISA) for detection of anti – Fasciola gigantica antibodies in sheep |
| title_fullStr | Evaluation of an in-house enzyme linked immuonosorbent assay (ELISA) for detection of anti – Fasciola gigantica antibodies in sheep |
| title_full_unstemmed | Evaluation of an in-house enzyme linked immuonosorbent assay (ELISA) for detection of anti – Fasciola gigantica antibodies in sheep |
| title_short | Evaluation of an in-house enzyme linked immuonosorbent assay (ELISA) for detection of anti – Fasciola gigantica antibodies in sheep |
| title_sort | evaluation of an in house enzyme linked immuonosorbent assay elisa for detection of anti fasciola gigantica antibodies in sheep |
| topic | fasciola gigantic elisa sheep |
| url | https://ijvst.um.ac.ir/article_26571_a3e05789909c4162baf685dd5e0e1c3f.pdf |
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