A miniature illumination probe in lightsheet fluorescence microscopy for real-time imaging injured adult zebrafish’s ventricular recuperation
AbstractImaging adult zebrafish’s cardiac organ is often hampered by the limited volume of the internal cavity. This research study aims to design, manufacture and apply a miniature illumination probe amended with an aluminum-coated polydimethylsiloxane (PDMS) concave cylindrical mirrors as well as...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | International Journal of Optomechatronics |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/15599612.2023.2293284 |
| _version_ | 1797369723826995200 |
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| author | Ho-Ching Hsiao Po-Sheng Hu |
| author_facet | Ho-Ching Hsiao Po-Sheng Hu |
| author_sort | Ho-Ching Hsiao |
| collection | DOAJ |
| description | AbstractImaging adult zebrafish’s cardiac organ is often hampered by the limited volume of the internal cavity. This research study aims to design, manufacture and apply a miniature illumination probe amended with an aluminum-coated polydimethylsiloxane (PDMS) concave cylindrical mirrors as well as 3D-printed casings for probe assembly in a light-sheet fluorescence microscopy to explore its applicability of visualizing adult zebrafish’s ventricle. The system was calibrated and characterized. The experimental results successfully demonstrated the tracking of the ventricular regeneration in female adult zebrafish at multiple time points and can be conducive to other heart-related research in in vivo settings. |
| first_indexed | 2024-03-08T17:52:03Z |
| format | Article |
| id | doaj.art-4fe16601bd4746939a0f1f028d30236b |
| institution | Directory Open Access Journal |
| issn | 1559-9612 1559-9620 |
| language | English |
| last_indexed | 2024-03-08T17:52:03Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Optomechatronics |
| spelling | doaj.art-4fe16601bd4746939a0f1f028d30236b2024-01-02T07:26:58ZengTaylor & Francis GroupInternational Journal of Optomechatronics1559-96121559-96202024-12-0118110.1080/15599612.2023.2293284A miniature illumination probe in lightsheet fluorescence microscopy for real-time imaging injured adult zebrafish’s ventricular recuperationHo-Ching Hsiao0Po-Sheng Hu1College of Photonics, National Yang Ming Chiao Tung University, Tainan City, TaiwanCollege of Photonics, National Yang Ming Chiao Tung University, Tainan City, TaiwanAbstractImaging adult zebrafish’s cardiac organ is often hampered by the limited volume of the internal cavity. This research study aims to design, manufacture and apply a miniature illumination probe amended with an aluminum-coated polydimethylsiloxane (PDMS) concave cylindrical mirrors as well as 3D-printed casings for probe assembly in a light-sheet fluorescence microscopy to explore its applicability of visualizing adult zebrafish’s ventricle. The system was calibrated and characterized. The experimental results successfully demonstrated the tracking of the ventricular regeneration in female adult zebrafish at multiple time points and can be conducive to other heart-related research in in vivo settings.https://www.tandfonline.com/doi/10.1080/15599612.2023.2293284Miniature illumination probelight-sheet fluorescence microscopy3D printingadult zebrafishin vivo monitoringventricular resection |
| spellingShingle | Ho-Ching Hsiao Po-Sheng Hu A miniature illumination probe in lightsheet fluorescence microscopy for real-time imaging injured adult zebrafish’s ventricular recuperation International Journal of Optomechatronics Miniature illumination probe light-sheet fluorescence microscopy 3D printing adult zebrafish in vivo monitoring ventricular resection |
| title | A miniature illumination probe in lightsheet fluorescence microscopy for real-time imaging injured adult zebrafish’s ventricular recuperation |
| title_full | A miniature illumination probe in lightsheet fluorescence microscopy for real-time imaging injured adult zebrafish’s ventricular recuperation |
| title_fullStr | A miniature illumination probe in lightsheet fluorescence microscopy for real-time imaging injured adult zebrafish’s ventricular recuperation |
| title_full_unstemmed | A miniature illumination probe in lightsheet fluorescence microscopy for real-time imaging injured adult zebrafish’s ventricular recuperation |
| title_short | A miniature illumination probe in lightsheet fluorescence microscopy for real-time imaging injured adult zebrafish’s ventricular recuperation |
| title_sort | miniature illumination probe in lightsheet fluorescence microscopy for real time imaging injured adult zebrafish s ventricular recuperation |
| topic | Miniature illumination probe light-sheet fluorescence microscopy 3D printing adult zebrafish in vivo monitoring ventricular resection |
| url | https://www.tandfonline.com/doi/10.1080/15599612.2023.2293284 |
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