Ocular Wnt/β-Catenin Pathway Inhibitor XAV939-Loaded Liposomes for Treating Alkali-Burned Corneal Wound and Neovascularization
Corneal wound involves a series of complex and coordinated physiological processes, leading to persistent epithelial defects and opacification. An obstacle in the treatment of ocular diseases is poor drug delivery and maintenance. In this study, we constructed a Wnt/β-catenin pathway inhibitor, XAV9...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.753879/full |
| _version_ | 1818726439090913280 |
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| author | Yueyang Zhong Yueyang Zhong Kai Wang Kai Wang Yin Zhang Yin Zhang Qichuan Yin Qichuan Yin Su Li Su Li Jiaming Wang Xiaobo Zhang Xiaobo Zhang Haijie Han Haijie Han Ke Yao Ke Yao |
| author_facet | Yueyang Zhong Yueyang Zhong Kai Wang Kai Wang Yin Zhang Yin Zhang Qichuan Yin Qichuan Yin Su Li Su Li Jiaming Wang Xiaobo Zhang Xiaobo Zhang Haijie Han Haijie Han Ke Yao Ke Yao |
| author_sort | Yueyang Zhong |
| collection | DOAJ |
| description | Corneal wound involves a series of complex and coordinated physiological processes, leading to persistent epithelial defects and opacification. An obstacle in the treatment of ocular diseases is poor drug delivery and maintenance. In this study, we constructed a Wnt/β-catenin pathway inhibitor, XAV939-loaded liposome (XAV939 NPs), and revealed its anti-inflammatory and antiangiogenic effects. The XAV939 NPs possessed excellent biocompatibility in corneal epithelial cells and mouse corneas. In vitro corneal wound healing assays demonstrated their antiangiogenic effect, and LPS-induced expressions of pro-inflammatory genes of IL-1β, IL-6, and IL-17α were significantly suppressed by XAV939 NPs. In addition, the XAV939 NPs significantly ameliorated alkali-burned corneas with slight corneal opacity, reduced neovascularization, and faster recovery, which were attributed to the decreased gene expressions of angiogenic and inflammatory cytokines. The findings supported the potential of XAV939 NPs in ameliorating corneal wound and suppressing neovascularization, providing evidence for their clinical application in ocular vascular diseases. |
| first_indexed | 2024-12-17T21:58:13Z |
| format | Article |
| id | doaj.art-4fe46abec4eb44ccae46432d29e00043 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-17T21:58:13Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-4fe46abec4eb44ccae46432d29e000432022-12-21T21:31:03ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-10-01910.3389/fbioe.2021.753879753879Ocular Wnt/β-Catenin Pathway Inhibitor XAV939-Loaded Liposomes for Treating Alkali-Burned Corneal Wound and NeovascularizationYueyang Zhong0Yueyang Zhong1Kai Wang2Kai Wang3Yin Zhang4Yin Zhang5Qichuan Yin6Qichuan Yin7Su Li8Su Li9Jiaming Wang10Xiaobo Zhang11Xiaobo Zhang12Haijie Han13Haijie Han14Ke Yao15Ke Yao16Eye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaZhejiang Provincial Key Lab of Ophthalmology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaEye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaZhejiang Provincial Key Lab of Ophthalmology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaEye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaZhejiang Provincial Key Lab of Ophthalmology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaEye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaZhejiang Provincial Key Lab of Ophthalmology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaEye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaZhejiang Provincial Key Lab of Ophthalmology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaThe First Affiliated Hospital, School of Public Health, Institute of Translational Medicine, State Key Laboratory of Experimental Hematology, School of Medicine, Zhejiang University, Hangzhou, ChinaEye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaZhejiang Provincial Key Lab of Ophthalmology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaEye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaZhejiang Provincial Key Lab of Ophthalmology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaEye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaZhejiang Provincial Key Lab of Ophthalmology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaCorneal wound involves a series of complex and coordinated physiological processes, leading to persistent epithelial defects and opacification. An obstacle in the treatment of ocular diseases is poor drug delivery and maintenance. In this study, we constructed a Wnt/β-catenin pathway inhibitor, XAV939-loaded liposome (XAV939 NPs), and revealed its anti-inflammatory and antiangiogenic effects. The XAV939 NPs possessed excellent biocompatibility in corneal epithelial cells and mouse corneas. In vitro corneal wound healing assays demonstrated their antiangiogenic effect, and LPS-induced expressions of pro-inflammatory genes of IL-1β, IL-6, and IL-17α were significantly suppressed by XAV939 NPs. In addition, the XAV939 NPs significantly ameliorated alkali-burned corneas with slight corneal opacity, reduced neovascularization, and faster recovery, which were attributed to the decreased gene expressions of angiogenic and inflammatory cytokines. The findings supported the potential of XAV939 NPs in ameliorating corneal wound and suppressing neovascularization, providing evidence for their clinical application in ocular vascular diseases.https://www.frontiersin.org/articles/10.3389/fbioe.2021.753879/fullliposomesWnt/β-catenin pathwayXAV939corneal woundcorneal neovascularization |
| spellingShingle | Yueyang Zhong Yueyang Zhong Kai Wang Kai Wang Yin Zhang Yin Zhang Qichuan Yin Qichuan Yin Su Li Su Li Jiaming Wang Xiaobo Zhang Xiaobo Zhang Haijie Han Haijie Han Ke Yao Ke Yao Ocular Wnt/β-Catenin Pathway Inhibitor XAV939-Loaded Liposomes for Treating Alkali-Burned Corneal Wound and Neovascularization Frontiers in Bioengineering and Biotechnology liposomes Wnt/β-catenin pathway XAV939 corneal wound corneal neovascularization |
| title | Ocular Wnt/β-Catenin Pathway Inhibitor XAV939-Loaded Liposomes for Treating Alkali-Burned Corneal Wound and Neovascularization |
| title_full | Ocular Wnt/β-Catenin Pathway Inhibitor XAV939-Loaded Liposomes for Treating Alkali-Burned Corneal Wound and Neovascularization |
| title_fullStr | Ocular Wnt/β-Catenin Pathway Inhibitor XAV939-Loaded Liposomes for Treating Alkali-Burned Corneal Wound and Neovascularization |
| title_full_unstemmed | Ocular Wnt/β-Catenin Pathway Inhibitor XAV939-Loaded Liposomes for Treating Alkali-Burned Corneal Wound and Neovascularization |
| title_short | Ocular Wnt/β-Catenin Pathway Inhibitor XAV939-Loaded Liposomes for Treating Alkali-Burned Corneal Wound and Neovascularization |
| title_sort | ocular wnt β catenin pathway inhibitor xav939 loaded liposomes for treating alkali burned corneal wound and neovascularization |
| topic | liposomes Wnt/β-catenin pathway XAV939 corneal wound corneal neovascularization |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.753879/full |
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