The Type III Accessory Protein HrpE of <em>Xanthomonas oryzae</em> pv. <em>oryzae</em> Surpasses the Secretion Role, and Enhances Plant Resistance and Photosynthesis
Many species of plant-pathogenic gram-negative bacteria deploy the type III (T3) secretion system to secrete virulence components, which are mostly characteristic of protein effectors targeting the cytosol of the plant cell following secretion. <i>Xanthomonas oryzae</i> pv. <i>oryz...
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MDPI AG
2019-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/11/572 |
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| author | Taha Majid Mahmood Sheikh Liyuan Zhang Muhammad Zubair Alvina Hanif Ping Li Ayaz Farzand Haider Ali Muhammad Saqib Bilal Yiqun Hu Xiaochen Chen Congfeng Song Hansong Dong Meixiang Zhang |
| author_facet | Taha Majid Mahmood Sheikh Liyuan Zhang Muhammad Zubair Alvina Hanif Ping Li Ayaz Farzand Haider Ali Muhammad Saqib Bilal Yiqun Hu Xiaochen Chen Congfeng Song Hansong Dong Meixiang Zhang |
| author_sort | Taha Majid Mahmood Sheikh |
| collection | DOAJ |
| description | Many species of plant-pathogenic gram-negative bacteria deploy the type III (T3) secretion system to secrete virulence components, which are mostly characteristic of protein effectors targeting the cytosol of the plant cell following secretion. <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>), a rice pathogen causing bacterial blight disease, uses the T3 accessory protein HrpE to assemble the pilus pathway, which in turn secretes transcription activator-like (TAL) effectors. The <i>hrpE</i> gene can execute extensive physiological and pathological functions beyond effector secretion. As evidenced in this study, when the <i>hrpE</i> gene was deleted from the <i>Xoo</i> genome, the bacteria incur seriouimpairments in multiplication, motility, and virulence. The virulence nullification is attributed to reduced secretion and translocation of PthXo1, which is a TAL effector that determines the bacterial virulence in the susceptible rice varieties. When the HrpE protein produced by prokaryotic expression is applied to plants, the recombinant protein is highly effective at inducing the defense response. Moreover, leaf photosynthesis efficiency is enhanced in HrpE-treated plants. These results provide experimental avenues to modulate the plant defense and growth tradeoff by manipulating a bacterial T3 accessory protein. |
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| last_indexed | 2024-12-12T10:22:53Z |
| publishDate | 2019-11-01 |
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| spelling | doaj.art-4fe53ac26c1241f5ad1a7035a99da2c52022-12-22T00:27:31ZengMDPI AGMicroorganisms2076-26072019-11-0171157210.3390/microorganisms7110572microorganisms7110572The Type III Accessory Protein HrpE of <em>Xanthomonas oryzae</em> pv. <em>oryzae</em> Surpasses the Secretion Role, and Enhances Plant Resistance and PhotosynthesisTaha Majid Mahmood Sheikh0Liyuan Zhang1Muhammad Zubair2Alvina Hanif3Ping Li4Ayaz Farzand5Haider Ali6Muhammad Saqib Bilal7Yiqun Hu8Xiaochen Chen9Congfeng Song10Hansong Dong11Meixiang Zhang12Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, Shandong Agricultural University, Taian 271018, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaMany species of plant-pathogenic gram-negative bacteria deploy the type III (T3) secretion system to secrete virulence components, which are mostly characteristic of protein effectors targeting the cytosol of the plant cell following secretion. <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>), a rice pathogen causing bacterial blight disease, uses the T3 accessory protein HrpE to assemble the pilus pathway, which in turn secretes transcription activator-like (TAL) effectors. The <i>hrpE</i> gene can execute extensive physiological and pathological functions beyond effector secretion. As evidenced in this study, when the <i>hrpE</i> gene was deleted from the <i>Xoo</i> genome, the bacteria incur seriouimpairments in multiplication, motility, and virulence. The virulence nullification is attributed to reduced secretion and translocation of PthXo1, which is a TAL effector that determines the bacterial virulence in the susceptible rice varieties. When the HrpE protein produced by prokaryotic expression is applied to plants, the recombinant protein is highly effective at inducing the defense response. Moreover, leaf photosynthesis efficiency is enhanced in HrpE-treated plants. These results provide experimental avenues to modulate the plant defense and growth tradeoff by manipulating a bacterial T3 accessory protein.https://www.mdpi.com/2076-2607/7/11/572hrpetype iii secretion systemeffector secretionvirulenceimmunity |
| spellingShingle | Taha Majid Mahmood Sheikh Liyuan Zhang Muhammad Zubair Alvina Hanif Ping Li Ayaz Farzand Haider Ali Muhammad Saqib Bilal Yiqun Hu Xiaochen Chen Congfeng Song Hansong Dong Meixiang Zhang The Type III Accessory Protein HrpE of <em>Xanthomonas oryzae</em> pv. <em>oryzae</em> Surpasses the Secretion Role, and Enhances Plant Resistance and Photosynthesis Microorganisms hrpe type iii secretion system effector secretion virulence immunity |
| title | The Type III Accessory Protein HrpE of <em>Xanthomonas oryzae</em> pv. <em>oryzae</em> Surpasses the Secretion Role, and Enhances Plant Resistance and Photosynthesis |
| title_full | The Type III Accessory Protein HrpE of <em>Xanthomonas oryzae</em> pv. <em>oryzae</em> Surpasses the Secretion Role, and Enhances Plant Resistance and Photosynthesis |
| title_fullStr | The Type III Accessory Protein HrpE of <em>Xanthomonas oryzae</em> pv. <em>oryzae</em> Surpasses the Secretion Role, and Enhances Plant Resistance and Photosynthesis |
| title_full_unstemmed | The Type III Accessory Protein HrpE of <em>Xanthomonas oryzae</em> pv. <em>oryzae</em> Surpasses the Secretion Role, and Enhances Plant Resistance and Photosynthesis |
| title_short | The Type III Accessory Protein HrpE of <em>Xanthomonas oryzae</em> pv. <em>oryzae</em> Surpasses the Secretion Role, and Enhances Plant Resistance and Photosynthesis |
| title_sort | type iii accessory protein hrpe of em xanthomonas oryzae em pv em oryzae em surpasses the secretion role and enhances plant resistance and photosynthesis |
| topic | hrpe type iii secretion system effector secretion virulence immunity |
| url | https://www.mdpi.com/2076-2607/7/11/572 |
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