NGF protects neuroblastoma cells against β‐amyloid‐induced apoptosis via the Nrf2/HO‐1 pathway
As one of the main neurotrophic factors, nerve growth factor (NGF) participates in various processes related to viability, plasticity, and neuronal growth. NGF is known to protect against cell death and toxicity triggered by β‐amyloid (Aβ), but the underlying mechanism remains unclear. Here, we inve...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-12-01
|
| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.12742 |
| _version_ | 1797542875269955584 |
|---|---|
| author | Rui Su Wei Su Qian Jiao |
| author_facet | Rui Su Wei Su Qian Jiao |
| author_sort | Rui Su |
| collection | DOAJ |
| description | As one of the main neurotrophic factors, nerve growth factor (NGF) participates in various processes related to viability, plasticity, and neuronal growth. NGF is known to protect against cell death and toxicity triggered by β‐amyloid (Aβ), but the underlying mechanism remains unclear. Here, we investigated this process in SKNSH neuroblastoma, in which NGF reduced cell death induced by Aβ25–35. Furthermore, NGF suppressed the production of reactive oxygen species (ROS) and promoted antioxidant function via Aβ25–35. Additionally, we demonstrated that NGF impaired the activation of the JNK/c‐Jun signaling pathway and significantly increased Nrf2 nuclear translocation and HO‐1 expression. Nrf2 elimination abolished the protective effect of NGF‐1 on Aβ25–35‐induced ROS generation, apoptosis, and activation of the JNK/c‐Jun pathway. The results of our study indicate that NGF protects neuroblastoma against injury triggered by Aβ25–35 via suppression of ROS–JNK/c‐Jun pathway stimulation through the Nrf2/HO‐1 pathway. |
| first_indexed | 2024-03-10T13:37:40Z |
| format | Article |
| id | doaj.art-4fe69eb719a242a89d0157e571ac8a83 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-03-10T13:37:40Z |
| publishDate | 2019-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-4fe69eb719a242a89d0157e571ac8a832023-11-21T07:01:44ZengWileyFEBS Open Bio2211-54632019-12-019122063207110.1002/2211-5463.12742NGF protects neuroblastoma cells against β‐amyloid‐induced apoptosis via the Nrf2/HO‐1 pathwayRui Su0Wei Su1Qian Jiao2Department of Neurosurgery Luoyang Central Hospital Affiliated to Zhengzhou University ChinaDepartment of Intensive Care Unit Sir Run Run Shaw Hospital Affiliated by Zhejiang University School of Medicine Hangzhou ChinaDepartment of Anesthesia Surgery Sanmenxia Central Hospital ChinaAs one of the main neurotrophic factors, nerve growth factor (NGF) participates in various processes related to viability, plasticity, and neuronal growth. NGF is known to protect against cell death and toxicity triggered by β‐amyloid (Aβ), but the underlying mechanism remains unclear. Here, we investigated this process in SKNSH neuroblastoma, in which NGF reduced cell death induced by Aβ25–35. Furthermore, NGF suppressed the production of reactive oxygen species (ROS) and promoted antioxidant function via Aβ25–35. Additionally, we demonstrated that NGF impaired the activation of the JNK/c‐Jun signaling pathway and significantly increased Nrf2 nuclear translocation and HO‐1 expression. Nrf2 elimination abolished the protective effect of NGF‐1 on Aβ25–35‐induced ROS generation, apoptosis, and activation of the JNK/c‐Jun pathway. The results of our study indicate that NGF protects neuroblastoma against injury triggered by Aβ25–35 via suppression of ROS–JNK/c‐Jun pathway stimulation through the Nrf2/HO‐1 pathway.https://doi.org/10.1002/2211-5463.12742apoptosisJNK/c‐JunNGFNrf2β‐amyloid |
| spellingShingle | Rui Su Wei Su Qian Jiao NGF protects neuroblastoma cells against β‐amyloid‐induced apoptosis via the Nrf2/HO‐1 pathway FEBS Open Bio apoptosis JNK/c‐Jun NGF Nrf2 β‐amyloid |
| title | NGF protects neuroblastoma cells against β‐amyloid‐induced apoptosis via the Nrf2/HO‐1 pathway |
| title_full | NGF protects neuroblastoma cells against β‐amyloid‐induced apoptosis via the Nrf2/HO‐1 pathway |
| title_fullStr | NGF protects neuroblastoma cells against β‐amyloid‐induced apoptosis via the Nrf2/HO‐1 pathway |
| title_full_unstemmed | NGF protects neuroblastoma cells against β‐amyloid‐induced apoptosis via the Nrf2/HO‐1 pathway |
| title_short | NGF protects neuroblastoma cells against β‐amyloid‐induced apoptosis via the Nrf2/HO‐1 pathway |
| title_sort | ngf protects neuroblastoma cells against β amyloid induced apoptosis via the nrf2 ho 1 pathway |
| topic | apoptosis JNK/c‐Jun NGF Nrf2 β‐amyloid |
| url | https://doi.org/10.1002/2211-5463.12742 |
| work_keys_str_mv | AT ruisu ngfprotectsneuroblastomacellsagainstbamyloidinducedapoptosisviathenrf2ho1pathway AT weisu ngfprotectsneuroblastomacellsagainstbamyloidinducedapoptosisviathenrf2ho1pathway AT qianjiao ngfprotectsneuroblastomacellsagainstbamyloidinducedapoptosisviathenrf2ho1pathway |