Albumin Is a Component of the Esterase Status of Human Blood Plasma
The esterase status of blood plasma can claim to be one of the universal markers of various diseases; therefore, it deserves attention when searching for markers of the severity of COVID-19 and other infectious and non-infectious pathologies. When analyzing the esterase status of blood plasma, the e...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/12/10383 |
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| author | Daria A. Belinskaia Polina A. Voronina Polina I. Popova Natalia G. Voitenko Vladimir I. Shmurak Mikhail A. Vovk Tatiana I. Baranova Anastasia A. Batalova Ekaterina A. Korf Pavel V. Avdonin Richard O. Jenkins Nikolay V. Goncharov |
| author_facet | Daria A. Belinskaia Polina A. Voronina Polina I. Popova Natalia G. Voitenko Vladimir I. Shmurak Mikhail A. Vovk Tatiana I. Baranova Anastasia A. Batalova Ekaterina A. Korf Pavel V. Avdonin Richard O. Jenkins Nikolay V. Goncharov |
| author_sort | Daria A. Belinskaia |
| collection | DOAJ |
| description | The esterase status of blood plasma can claim to be one of the universal markers of various diseases; therefore, it deserves attention when searching for markers of the severity of COVID-19 and other infectious and non-infectious pathologies. When analyzing the esterase status of blood plasma, the esterase activity of serum albumin, which is the major protein in the blood of mammals, should not be ignored. The purpose of this study is to expand understanding of the esterase status of blood plasma and to evaluate the relationship of the esterase status, which includes information on the amount and enzymatic activity of human serum albumin (HSA), with other biochemical parameters of human blood, using the example of surviving and deceased patients with confirmed COVID-19. In experiments in vitro and in silico, the activity of human plasma and pure HSA towards various substrates was studied, and the effect of various inhibitors on this activity was tested. Then, a comparative analysis of the esterase status and a number of basic biochemical parameters of the blood plasma of healthy subjects and patients with confirmed COVID-19 was performed. Statistically significant differences have been found in esterase status and biochemical indices (including albumin levels) between healthy subjects and patients with COVID-19, as well as between surviving and deceased patients. Additional evidence has been obtained for the importance of albumin as a diagnostic marker. Of particular interest is a new index, [Urea] × [MDA] × 1000/(BChEb × [ALB]), which in the group of deceased patients was 10 times higher than in the group of survivors and 26 times higher than the value in the group of apparently healthy elderly subjects. |
| first_indexed | 2024-03-11T02:20:18Z |
| format | Article |
| id | doaj.art-4fe764eb98d34714a3e33a862abb33c4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T02:20:18Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4fe764eb98d34714a3e33a862abb33c42023-11-18T10:52:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-0124121038310.3390/ijms241210383Albumin Is a Component of the Esterase Status of Human Blood PlasmaDaria A. Belinskaia0Polina A. Voronina1Polina I. Popova2Natalia G. Voitenko3Vladimir I. Shmurak4Mikhail A. Vovk5Tatiana I. Baranova6Anastasia A. Batalova7Ekaterina A. Korf8Pavel V. Avdonin9Richard O. Jenkins10Nikolay V. Goncharov11Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, pr. Torez 44, 194223 St. Petersburg, RussiaSechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, pr. Torez 44, 194223 St. Petersburg, RussiaCity Polyclinic No. 112, 25 Academician Baykov Str., 195427 St. Petersburg, RussiaSechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, pr. Torez 44, 194223 St. Petersburg, RussiaSechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, pr. Torez 44, 194223 St. Petersburg, RussiaCentre for Magnetic Resonance, St. Petersburg State University, Universitetskij pr., 26, Peterhof, 198504 St. Petersburg, RussiaFaculty of Biology, St. Petersburg State University, 7-9 Universitetskaya Emb., 199034 St. Petersburg, RussiaSechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, pr. Torez 44, 194223 St. Petersburg, RussiaSechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, pr. Torez 44, 194223 St. Petersburg, RussiaKoltsov Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilova Str., 119334 Moscow, RussiaLeicester School of Allied Health Sciences, De Montfort University, The Gateway, Leicester LE1 9BH, UKSechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, pr. Torez 44, 194223 St. Petersburg, RussiaThe esterase status of blood plasma can claim to be one of the universal markers of various diseases; therefore, it deserves attention when searching for markers of the severity of COVID-19 and other infectious and non-infectious pathologies. When analyzing the esterase status of blood plasma, the esterase activity of serum albumin, which is the major protein in the blood of mammals, should not be ignored. The purpose of this study is to expand understanding of the esterase status of blood plasma and to evaluate the relationship of the esterase status, which includes information on the amount and enzymatic activity of human serum albumin (HSA), with other biochemical parameters of human blood, using the example of surviving and deceased patients with confirmed COVID-19. In experiments in vitro and in silico, the activity of human plasma and pure HSA towards various substrates was studied, and the effect of various inhibitors on this activity was tested. Then, a comparative analysis of the esterase status and a number of basic biochemical parameters of the blood plasma of healthy subjects and patients with confirmed COVID-19 was performed. Statistically significant differences have been found in esterase status and biochemical indices (including albumin levels) between healthy subjects and patients with COVID-19, as well as between surviving and deceased patients. Additional evidence has been obtained for the importance of albumin as a diagnostic marker. Of particular interest is a new index, [Urea] × [MDA] × 1000/(BChEb × [ALB]), which in the group of deceased patients was 10 times higher than in the group of survivors and 26 times higher than the value in the group of apparently healthy elderly subjects.https://www.mdpi.com/1422-0067/24/12/10383albuminbiomarkercholinesterasecarboxylesteraseCOVID-19diagnostics |
| spellingShingle | Daria A. Belinskaia Polina A. Voronina Polina I. Popova Natalia G. Voitenko Vladimir I. Shmurak Mikhail A. Vovk Tatiana I. Baranova Anastasia A. Batalova Ekaterina A. Korf Pavel V. Avdonin Richard O. Jenkins Nikolay V. Goncharov Albumin Is a Component of the Esterase Status of Human Blood Plasma International Journal of Molecular Sciences albumin biomarker cholinesterase carboxylesterase COVID-19 diagnostics |
| title | Albumin Is a Component of the Esterase Status of Human Blood Plasma |
| title_full | Albumin Is a Component of the Esterase Status of Human Blood Plasma |
| title_fullStr | Albumin Is a Component of the Esterase Status of Human Blood Plasma |
| title_full_unstemmed | Albumin Is a Component of the Esterase Status of Human Blood Plasma |
| title_short | Albumin Is a Component of the Esterase Status of Human Blood Plasma |
| title_sort | albumin is a component of the esterase status of human blood plasma |
| topic | albumin biomarker cholinesterase carboxylesterase COVID-19 diagnostics |
| url | https://www.mdpi.com/1422-0067/24/12/10383 |
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