ANTI-CANCER ACTIVITY OF GRAVIOLA (ANNONA MURICATA) LEAVES EXTRACT ON INDUCED BREAST CANCER IN RATS’ MODEL
Breast cancer is the most frequent type of invasive cancer in women. However, chemotherapy affects all cells that grow and divide quickly in the body, including cancer and normal cells. On the other hand, Graviola is commonly used as a source of food and has a wide range of bioactive components. In...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2023-01-01
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| Series: | Slovenian Veterinary Research |
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| Online Access: | https://www.slovetres.si/index.php/SVR/article/view/1573 |
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| author | Areej Salem Hosny Abd El-Fadil Nagaah Al-Sayed Ahmed S. Alazzouni Sameh El-Nabtity |
| author_facet | Areej Salem Hosny Abd El-Fadil Nagaah Al-Sayed Ahmed S. Alazzouni Sameh El-Nabtity |
| author_sort | Areej Salem |
| collection | DOAJ |
| description |
Breast cancer is the most frequent type of invasive cancer in women. However, chemotherapy affects all cells that grow and divide quickly in the body, including cancer and normal cells. On the other hand, Graviola is commonly used as a source of food and has a wide range of bioactive components. In this study, out of 50 albino rats, breast cancer was induced in 40 rats using 7,12-dimethylbenanthracene (DMBA). These rats were subjected to the treatment using Graviola leaves ethanolic extract (GLEE) and 5-Fluorouracil (5-FU) to evaluate the anti-carcinogenic activity and the immunohistochemical changes. A single oral dose of DMBA led to the induction of rats’ breast cancer. Induced mammary tumors were diagnosed, and all were malignant without any benign tumor. Among the malignant tumors, non-invasive, invasive, mixed (invasive and non-invasive), and unclassified malignant tumors were detected. The immunohistochemical analysis showed that estrogen and progesterone receptors revealed negative nuclear expression. However, HER2 receptors score was +3, and Ki-67 revealed 85-90% nuclear stainability, denoting a very high proliferative index. The morphometric analysis revealed that staining reactivities of HER2 and Ki-67 were 60.66 and 89.84%, respectively, and were significantly higher than ER (15.24 %) and PR (15.68%). Genetic studies revealed marked upregulation of P53 with GLEE more than 5-FU while Bcl2 showed down regulation with GLEE more than 5-FU. The quantitative analysis of GLEE phytochemical constituents showed the presence of some bioactive chemical compounds that exhibit many therapeutic activities. Therefore, GLEE improved histological appearance of DMBA induced breast cancer in female rats. Therefore, GLEE could be a promising natural alternative to chemotherapy agents as a potent anticancer.
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| first_indexed | 2024-04-24T14:02:07Z |
| format | Article |
| id | doaj.art-4fe776e19c864f05b66293100edcbd64 |
| institution | Directory Open Access Journal |
| issn | 1580-4003 2385-8761 |
| language | English |
| last_indexed | 2024-04-24T14:02:07Z |
| publishDate | 2023-01-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Slovenian Veterinary Research |
| spelling | doaj.art-4fe776e19c864f05b66293100edcbd642024-04-03T12:36:49ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Slovenian Veterinary Research1580-40032385-87612023-01-016025-Suppl10.26873/SVR-1573-2022ANTI-CANCER ACTIVITY OF GRAVIOLA (ANNONA MURICATA) LEAVES EXTRACT ON INDUCED BREAST CANCER IN RATS’ MODELAreej Salem0Hosny Abd El-Fadil1Nagaah Al-Sayed2Ahmed S. Alazzouni3Sameh El-Nabtity4Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Egypt, Corresponding author, E-mail: areejibrahim3@gmail.comDepartment of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, EgyptDepartment of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, EgyptDepartment of Zoology, Faculty of Science, Helwan University, EgyptDepartment of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Egypt Breast cancer is the most frequent type of invasive cancer in women. However, chemotherapy affects all cells that grow and divide quickly in the body, including cancer and normal cells. On the other hand, Graviola is commonly used as a source of food and has a wide range of bioactive components. In this study, out of 50 albino rats, breast cancer was induced in 40 rats using 7,12-dimethylbenanthracene (DMBA). These rats were subjected to the treatment using Graviola leaves ethanolic extract (GLEE) and 5-Fluorouracil (5-FU) to evaluate the anti-carcinogenic activity and the immunohistochemical changes. A single oral dose of DMBA led to the induction of rats’ breast cancer. Induced mammary tumors were diagnosed, and all were malignant without any benign tumor. Among the malignant tumors, non-invasive, invasive, mixed (invasive and non-invasive), and unclassified malignant tumors were detected. The immunohistochemical analysis showed that estrogen and progesterone receptors revealed negative nuclear expression. However, HER2 receptors score was +3, and Ki-67 revealed 85-90% nuclear stainability, denoting a very high proliferative index. The morphometric analysis revealed that staining reactivities of HER2 and Ki-67 were 60.66 and 89.84%, respectively, and were significantly higher than ER (15.24 %) and PR (15.68%). Genetic studies revealed marked upregulation of P53 with GLEE more than 5-FU while Bcl2 showed down regulation with GLEE more than 5-FU. The quantitative analysis of GLEE phytochemical constituents showed the presence of some bioactive chemical compounds that exhibit many therapeutic activities. Therefore, GLEE improved histological appearance of DMBA induced breast cancer in female rats. Therefore, GLEE could be a promising natural alternative to chemotherapy agents as a potent anticancer. https://www.slovetres.si/index.php/SVR/article/view/1573breast cancergraviolaphytochemicalDMBA5-FlurouracilP53 |
| spellingShingle | Areej Salem Hosny Abd El-Fadil Nagaah Al-Sayed Ahmed S. Alazzouni Sameh El-Nabtity ANTI-CANCER ACTIVITY OF GRAVIOLA (ANNONA MURICATA) LEAVES EXTRACT ON INDUCED BREAST CANCER IN RATS’ MODEL Slovenian Veterinary Research breast cancer graviola phytochemical DMBA 5-Flurouracil P53 |
| title | ANTI-CANCER ACTIVITY OF GRAVIOLA (ANNONA MURICATA) LEAVES EXTRACT ON INDUCED BREAST CANCER IN RATS’ MODEL |
| title_full | ANTI-CANCER ACTIVITY OF GRAVIOLA (ANNONA MURICATA) LEAVES EXTRACT ON INDUCED BREAST CANCER IN RATS’ MODEL |
| title_fullStr | ANTI-CANCER ACTIVITY OF GRAVIOLA (ANNONA MURICATA) LEAVES EXTRACT ON INDUCED BREAST CANCER IN RATS’ MODEL |
| title_full_unstemmed | ANTI-CANCER ACTIVITY OF GRAVIOLA (ANNONA MURICATA) LEAVES EXTRACT ON INDUCED BREAST CANCER IN RATS’ MODEL |
| title_short | ANTI-CANCER ACTIVITY OF GRAVIOLA (ANNONA MURICATA) LEAVES EXTRACT ON INDUCED BREAST CANCER IN RATS’ MODEL |
| title_sort | anti cancer activity of graviola annona muricata leaves extract on induced breast cancer in rats model |
| topic | breast cancer graviola phytochemical DMBA 5-Flurouracil P53 |
| url | https://www.slovetres.si/index.php/SVR/article/view/1573 |
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