Design and Performance of an Automated Bioreactor for Cell Culture Experiments in a Microgravity Environment
In this paper, we describe the development of a bioreactor for a cell-culture experiment on the International Space Station (ISS). The bioreactor is an experimental device for culturing mouse muscle cells in a microgravity environment. The purpose of the experiment was to assess the impact of micr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The Korean Space Science Society
2015-03-01
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| Series: | Journal of Astronomy and Space Sciences |
| Subjects: | |
| Online Access: | http://ocean.kisti.re.kr/downfile/volume/kosss/OJOOBS/2015/v32n1/OJOOBS_2015_v32n1_81.pdf |
| _version_ | 1827393967092662272 |
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| author | Youn-Kyu Kim Seul-Hyun Park Joo-Hee Lee Gi-Hyuk Choi |
| author_facet | Youn-Kyu Kim Seul-Hyun Park Joo-Hee Lee Gi-Hyuk Choi |
| author_sort | Youn-Kyu Kim |
| collection | DOAJ |
| description | In this paper, we describe the development of a bioreactor for a cell-culture experiment on the International Space
Station (ISS). The bioreactor is an experimental device for culturing mouse muscle cells in a microgravity environment.
The purpose of the experiment was to assess the impact of microgravity on the muscles to address the possibility of longterm
human residence in space. After investigation of previously developed bioreactors, and analysis of the requirements
for microgravity cell culture experiments, a bioreactor design is herein proposed that is able to automatically culture 32
samples simultaneously. This reactor design is capable of automatic control of temperature, humidity, and culture-medium
injection rate; and satisfies the interface requirements of the ISS. Since bioreactors are vulnerable to cell contamination,
the medium-circulation modules were designed to be a completely replaceable, in order to reuse the bioreactor after each
experiment. The bioreactor control system is designed to circulate culture media to 32 culture chambers at a maximum
speed of 1 ml/min, to maintain the temperature of the reactor at 36±1°C, and to keep the relative humidity of the reactor
above 70%. Because bubbles in the culture media negatively affect cell culture, a de-bubbler unit was provided to eliminate
such bubbles. A working model of the reactor was built according to the new design, to verify its performance, and was used
to perform a cell culture experiment that confirmed the feasibility of this device. |
| first_indexed | 2024-03-08T17:59:09Z |
| format | Article |
| id | doaj.art-4fe931e3bb924003bb82e5adac6ed722 |
| institution | Directory Open Access Journal |
| issn | 2093-5587 2093-1409 |
| language | English |
| last_indexed | 2024-03-08T17:59:09Z |
| publishDate | 2015-03-01 |
| publisher | The Korean Space Science Society |
| record_format | Article |
| series | Journal of Astronomy and Space Sciences |
| spelling | doaj.art-4fe931e3bb924003bb82e5adac6ed7222024-01-02T02:32:19ZengThe Korean Space Science SocietyJournal of Astronomy and Space Sciences2093-55872093-14092015-03-01321818910.5140/JASS.2015.32.1.81Design and Performance of an Automated Bioreactor for Cell Culture Experiments in a Microgravity EnvironmentYoun-Kyu Kim0Seul-Hyun Park1Joo-Hee Lee2Gi-Hyuk Choi3Convergence Technology Research Head Office, Korea Aerospace Research Institute, 305-806, KoreaDepartment of Mechanical Design Engineering, Chosun University, 501-759, KoreaConvergence Technology Research Head Office, Korea Aerospace Research Institute, 305-806, KoreaConvergence Technology Research Head Office, Korea Aerospace Research Institute, 305-806, KoreaIn this paper, we describe the development of a bioreactor for a cell-culture experiment on the International Space Station (ISS). The bioreactor is an experimental device for culturing mouse muscle cells in a microgravity environment. The purpose of the experiment was to assess the impact of microgravity on the muscles to address the possibility of longterm human residence in space. After investigation of previously developed bioreactors, and analysis of the requirements for microgravity cell culture experiments, a bioreactor design is herein proposed that is able to automatically culture 32 samples simultaneously. This reactor design is capable of automatic control of temperature, humidity, and culture-medium injection rate; and satisfies the interface requirements of the ISS. Since bioreactors are vulnerable to cell contamination, the medium-circulation modules were designed to be a completely replaceable, in order to reuse the bioreactor after each experiment. The bioreactor control system is designed to circulate culture media to 32 culture chambers at a maximum speed of 1 ml/min, to maintain the temperature of the reactor at 36±1°C, and to keep the relative humidity of the reactor above 70%. Because bubbles in the culture media negatively affect cell culture, a de-bubbler unit was provided to eliminate such bubbles. A working model of the reactor was built according to the new design, to verify its performance, and was used to perform a cell culture experiment that confirmed the feasibility of this device.http://ocean.kisti.re.kr/downfile/volume/kosss/OJOOBS/2015/v32n1/OJOOBS_2015_v32n1_81.pdfspace experimentInternational Space Station (ISS) |
| spellingShingle | Youn-Kyu Kim Seul-Hyun Park Joo-Hee Lee Gi-Hyuk Choi Design and Performance of an Automated Bioreactor for Cell Culture Experiments in a Microgravity Environment Journal of Astronomy and Space Sciences space experiment International Space Station (ISS) |
| title | Design and Performance of an Automated Bioreactor for Cell Culture Experiments in a Microgravity Environment |
| title_full | Design and Performance of an Automated Bioreactor for Cell Culture Experiments in a Microgravity Environment |
| title_fullStr | Design and Performance of an Automated Bioreactor for Cell Culture Experiments in a Microgravity Environment |
| title_full_unstemmed | Design and Performance of an Automated Bioreactor for Cell Culture Experiments in a Microgravity Environment |
| title_short | Design and Performance of an Automated Bioreactor for Cell Culture Experiments in a Microgravity Environment |
| title_sort | design and performance of an automated bioreactor for cell culture experiments in a microgravity environment |
| topic | space experiment International Space Station (ISS) |
| url | http://ocean.kisti.re.kr/downfile/volume/kosss/OJOOBS/2015/v32n1/OJOOBS_2015_v32n1_81.pdf |
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