New methods for the diagnosis of Babesia bigemina infection
Accurate diagnosis of Babesia bigemina infection, an economically important tick-transmitted protozoan parasite of cattle, is essential in the management of disease control and in epidemiological studies. The currentlyused methods of diagnosis are blood smear examination and serological tests which...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1992-01-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000700033 |
| _version_ | 1797712598747054080 |
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| author | S. Morzaria J. Katende A. Kairo V. Nene A. Musoke |
| author_facet | S. Morzaria J. Katende A. Kairo V. Nene A. Musoke |
| author_sort | S. Morzaria |
| collection | DOAJ |
| description | Accurate diagnosis of Babesia bigemina infection, an economically important tick-transmitted protozoan parasite of cattle, is essential in the management of disease control and in epidemiological studies. The currentlyused methods of diagnosis are blood smear examination and serological tests which include agglutination and immunofluorescence tests. These testes have been used the fild but because they lack sensitivity and specificity, never and improved methods of diagnosis are being developed. The quantitative buffy coat (OBC) method, using microhaematocrit tubes and acridine orange staining allows rapid and quicker diagnosis of B. bigemina and other blood parasites compared to light microscopic examination of stained smears. Parasite specific monoclonal antibodies have been used in antigen/antibody capture enzymelinked immunosorbent assays with grater sensitivity and specificity than previously described serological tests. Similary, DNA probes, derived from a repetitive sequence of the B. bigemina genome, offer a method of detecting very small numbers of parasites which are undetectable by conventional microscopy. An extrachromosomal DNA element, present in all the tick-borne protozoan parasites so far tested, provides an accurate means of diferentiating mixed parasite populations in infected animals. These improved methods will greatly facilitate epidemiological studies. |
| first_indexed | 2024-03-12T07:24:08Z |
| format | Article |
| id | doaj.art-4fe992026ff647a58d994bb78fb7012f |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:24:08Z |
| publishDate | 1992-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-4fe992026ff647a58d994bb78fb7012f2023-09-02T22:14:52ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601992-01-018720120510.1590/S0074-02761992000700033New methods for the diagnosis of Babesia bigemina infectionS. MorzariaJ. KatendeA. KairoV. NeneA. MusokeAccurate diagnosis of Babesia bigemina infection, an economically important tick-transmitted protozoan parasite of cattle, is essential in the management of disease control and in epidemiological studies. The currentlyused methods of diagnosis are blood smear examination and serological tests which include agglutination and immunofluorescence tests. These testes have been used the fild but because they lack sensitivity and specificity, never and improved methods of diagnosis are being developed. The quantitative buffy coat (OBC) method, using microhaematocrit tubes and acridine orange staining allows rapid and quicker diagnosis of B. bigemina and other blood parasites compared to light microscopic examination of stained smears. Parasite specific monoclonal antibodies have been used in antigen/antibody capture enzymelinked immunosorbent assays with grater sensitivity and specificity than previously described serological tests. Similary, DNA probes, derived from a repetitive sequence of the B. bigemina genome, offer a method of detecting very small numbers of parasites which are undetectable by conventional microscopy. An extrachromosomal DNA element, present in all the tick-borne protozoan parasites so far tested, provides an accurate means of diferentiating mixed parasite populations in infected animals. These improved methods will greatly facilitate epidemiological studies.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000700033Babesia bigeminaantigensdiagnosisDNA probequantative buffy coat test |
| spellingShingle | S. Morzaria J. Katende A. Kairo V. Nene A. Musoke New methods for the diagnosis of Babesia bigemina infection Memorias do Instituto Oswaldo Cruz Babesia bigemina antigens diagnosis DNA probe quantative buffy coat test |
| title | New methods for the diagnosis of Babesia bigemina infection |
| title_full | New methods for the diagnosis of Babesia bigemina infection |
| title_fullStr | New methods for the diagnosis of Babesia bigemina infection |
| title_full_unstemmed | New methods for the diagnosis of Babesia bigemina infection |
| title_short | New methods for the diagnosis of Babesia bigemina infection |
| title_sort | new methods for the diagnosis of babesia bigemina infection |
| topic | Babesia bigemina antigens diagnosis DNA probe quantative buffy coat test |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000700033 |
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