O-GlcNAcylation of RIPK1 rescues red blood cells from necroptosis
Necroptosis is a type of cell death with excessive inflammation and organ damage in various human diseases. Although abnormal necroptosis is common in patients with neurodegenerative, cardiovascular, and infectious diseases, the mechanisms by which O-GlcNAcylation contributes to the regulation of ne...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1160490/full |
| _version_ | 1797808326969393152 |
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| author | Junghwa Seo Yeolhoe Kim Yeolhoe Kim Suena Ji Han Byeol Kim Hyeryeon Jung Hyeryeon Jung Eugene C. Yi Eugene C. Yi Yong-ho Lee Yong-ho Lee Injae Shin Injae Shin Won Ho Yang Won Ho Yang Jin Won Cho Jin Won Cho |
| author_facet | Junghwa Seo Yeolhoe Kim Yeolhoe Kim Suena Ji Han Byeol Kim Hyeryeon Jung Hyeryeon Jung Eugene C. Yi Eugene C. Yi Yong-ho Lee Yong-ho Lee Injae Shin Injae Shin Won Ho Yang Won Ho Yang Jin Won Cho Jin Won Cho |
| author_sort | Junghwa Seo |
| collection | DOAJ |
| description | Necroptosis is a type of cell death with excessive inflammation and organ damage in various human diseases. Although abnormal necroptosis is common in patients with neurodegenerative, cardiovascular, and infectious diseases, the mechanisms by which O-GlcNAcylation contributes to the regulation of necroptotic cell death are poorly understood. In this study, we reveal that O-GlcNAcylation of RIPK1 (receptor-interacting protein kinase1) was decreased in erythrocytes of the mouse injected with lipopolysaccharide, resulting in the acceleration of erythrocyte necroptosis through increased formation of RIPK1-RIPK3 complex. Mechanistically, we discovered that O-GlcNAcylation of RIPK1 at serine 331 in human (corresponding to serine 332 in mouse) inhibits phosphorylation of RIPK1 at serine 166, which is necessary for the necroptotic activity of RIPK1 and suppresses the formation of the RIPK1-RIPK3 complex in Ripk1-/- MEFs. Thus, our study demonstrates that RIPK1 O-GlcNAcylation serves as a checkpoint to suppress necroptotic signaling in erythrocytes. |
| first_indexed | 2024-03-13T06:35:54Z |
| format | Article |
| id | doaj.art-4fe9f76e86f5401db95a5a9f89b10938 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-13T06:35:54Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-4fe9f76e86f5401db95a5a9f89b109382023-06-09T05:09:56ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-06-011410.3389/fimmu.2023.11604901160490O-GlcNAcylation of RIPK1 rescues red blood cells from necroptosisJunghwa Seo0Yeolhoe Kim1Yeolhoe Kim2Suena Ji3Han Byeol Kim4Hyeryeon Jung5Hyeryeon Jung6Eugene C. Yi7Eugene C. Yi8Yong-ho Lee9Yong-ho Lee10Injae Shin11Injae Shin12Won Ho Yang13Won Ho Yang14Jin Won Cho15Jin Won Cho16Glycosylation Network Research Center, Yonsei University, Seoul, Republic of KoreaGlycosylation Network Research Center, Yonsei University, Seoul, Republic of KoreaDepartment of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of KoreaGlycosylation Network Research Center, Yonsei University, Seoul, Republic of KoreaDepartment of Molecular Medicine and Biopharmaceutical Sciences, School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National University, Seoul, Republic of KoreaGlycosylation Network Research Center, Yonsei University, Seoul, Republic of KoreaDepartment of Molecular Medicine and Biopharmaceutical Sciences, School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National University, Seoul, Republic of KoreaGlycosylation Network Research Center, Yonsei University, Seoul, Republic of KoreaDepartment of Molecular Medicine and Biopharmaceutical Sciences, School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National University, Seoul, Republic of KoreaGlycosylation Network Research Center, Yonsei University, Seoul, Republic of KoreaDepartment of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of KoreaGlycosylation Network Research Center, Yonsei University, Seoul, Republic of KoreaDepartment of Chemistry, Yonsei University, Seoul, Republic of KoreaGlycosylation Network Research Center, Yonsei University, Seoul, Republic of KoreaDepartment of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of KoreaGlycosylation Network Research Center, Yonsei University, Seoul, Republic of KoreaDepartment of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of KoreaNecroptosis is a type of cell death with excessive inflammation and organ damage in various human diseases. Although abnormal necroptosis is common in patients with neurodegenerative, cardiovascular, and infectious diseases, the mechanisms by which O-GlcNAcylation contributes to the regulation of necroptotic cell death are poorly understood. In this study, we reveal that O-GlcNAcylation of RIPK1 (receptor-interacting protein kinase1) was decreased in erythrocytes of the mouse injected with lipopolysaccharide, resulting in the acceleration of erythrocyte necroptosis through increased formation of RIPK1-RIPK3 complex. Mechanistically, we discovered that O-GlcNAcylation of RIPK1 at serine 331 in human (corresponding to serine 332 in mouse) inhibits phosphorylation of RIPK1 at serine 166, which is necessary for the necroptotic activity of RIPK1 and suppresses the formation of the RIPK1-RIPK3 complex in Ripk1-/- MEFs. Thus, our study demonstrates that RIPK1 O-GlcNAcylation serves as a checkpoint to suppress necroptotic signaling in erythrocytes.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1160490/fullnecroptosisreceptor interacting protein kinase1 (RIPK1)O-GlcNAcylationerythrocytered blood cell |
| spellingShingle | Junghwa Seo Yeolhoe Kim Yeolhoe Kim Suena Ji Han Byeol Kim Hyeryeon Jung Hyeryeon Jung Eugene C. Yi Eugene C. Yi Yong-ho Lee Yong-ho Lee Injae Shin Injae Shin Won Ho Yang Won Ho Yang Jin Won Cho Jin Won Cho O-GlcNAcylation of RIPK1 rescues red blood cells from necroptosis Frontiers in Immunology necroptosis receptor interacting protein kinase1 (RIPK1) O-GlcNAcylation erythrocyte red blood cell |
| title | O-GlcNAcylation of RIPK1 rescues red blood cells from necroptosis |
| title_full | O-GlcNAcylation of RIPK1 rescues red blood cells from necroptosis |
| title_fullStr | O-GlcNAcylation of RIPK1 rescues red blood cells from necroptosis |
| title_full_unstemmed | O-GlcNAcylation of RIPK1 rescues red blood cells from necroptosis |
| title_short | O-GlcNAcylation of RIPK1 rescues red blood cells from necroptosis |
| title_sort | o glcnacylation of ripk1 rescues red blood cells from necroptosis |
| topic | necroptosis receptor interacting protein kinase1 (RIPK1) O-GlcNAcylation erythrocyte red blood cell |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1160490/full |
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