Calcium-Based Antimicrobial Peptide Compounds Attenuate DNFB-Induced Atopic Dermatitis-Like Skin Lesions via Th-Cells in BALB/c Mice
Atopic dermatitis (AD) is a chronic and recurrent inflammatory skin disease, characterized by severe itching and recurrent skin lesions. We hypothesized that a novel treatment involving calcium-based antimicrobial peptide compounds (CAPCS), a combination of natural calcium extracted from marine shel...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/19/11371 |
| _version_ | 1797479030041083904 |
|---|---|
| author | Qingfeng Liu Mengmeng Li Na Wang Chun He Xian Jiang Jingyi Li |
| author_facet | Qingfeng Liu Mengmeng Li Na Wang Chun He Xian Jiang Jingyi Li |
| author_sort | Qingfeng Liu |
| collection | DOAJ |
| description | Atopic dermatitis (AD) is a chronic and recurrent inflammatory skin disease, characterized by severe itching and recurrent skin lesions. We hypothesized that a novel treatment involving calcium-based antimicrobial peptide compounds (CAPCS), a combination of natural calcium extracted from marine shellfish, and a variety of antimicrobial peptides, may be beneficial for AD. We established a dinitrofluorobenzene (DNFB)-induced AD model in BALB/c mice to test our hypothesis. We observed mouse behavior and conducted histopathological and immunohistochemical analyses on skin lesions before and after CAPCS treatment. We also characterized the changes in the levels of cytokines, inflammatory mediators, and Toll-like receptors (TLRs) in plasma and skin lesions. The results showed that (i) topical application of CAPCS ameliorated AD-like skin lesions and reduced scratching behavior in BALB/c mice; (ii) CAPCS suppressed infiltration of inflammatory cells and inhibited the expression of inflammatory cytokines in AD-like skin lesions; (iii) CAPCS reduced plasma levels of inflammatory cytokines; and (iv) CAPCS inhibited TLR2 and TLR4 protein expression in skin lesions. Topical application of CAPCS exhibits a therapeutic effect on AD by inhibiting inflammatory immune responses via recruiting helper T cells and engaging the TLR2 and TLR4 signaling pathways. Therefore, CAPCS may be useful for the treatment of AD. |
| first_indexed | 2024-03-09T21:40:03Z |
| format | Article |
| id | doaj.art-4feba1b8870c4a82b48c20e68fc0fc6d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:40:03Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4feba1b8870c4a82b48c20e68fc0fc6d2023-11-23T20:32:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123191137110.3390/ijms231911371Calcium-Based Antimicrobial Peptide Compounds Attenuate DNFB-Induced Atopic Dermatitis-Like Skin Lesions via Th-Cells in BALB/c MiceQingfeng Liu0Mengmeng Li1Na Wang2Chun He3Xian Jiang4Jingyi Li5Department of Dermatology and Venereology, West China Hospital of Sichuan University, Chengdu 610041, ChinaDepartment of Dermatology and Venereology, West China Hospital of Sichuan University, Chengdu 610041, ChinaDepartment of Dermatology and Venereology, West China Hospital of Sichuan University, Chengdu 610041, ChinaDepartment of Dermatology and Venereology, West China Hospital of Sichuan University, Chengdu 610041, ChinaDepartment of Dermatology and Venereology, West China Hospital of Sichuan University, Chengdu 610041, ChinaDepartment of Dermatology and Venereology, West China Hospital of Sichuan University, Chengdu 610041, ChinaAtopic dermatitis (AD) is a chronic and recurrent inflammatory skin disease, characterized by severe itching and recurrent skin lesions. We hypothesized that a novel treatment involving calcium-based antimicrobial peptide compounds (CAPCS), a combination of natural calcium extracted from marine shellfish, and a variety of antimicrobial peptides, may be beneficial for AD. We established a dinitrofluorobenzene (DNFB)-induced AD model in BALB/c mice to test our hypothesis. We observed mouse behavior and conducted histopathological and immunohistochemical analyses on skin lesions before and after CAPCS treatment. We also characterized the changes in the levels of cytokines, inflammatory mediators, and Toll-like receptors (TLRs) in plasma and skin lesions. The results showed that (i) topical application of CAPCS ameliorated AD-like skin lesions and reduced scratching behavior in BALB/c mice; (ii) CAPCS suppressed infiltration of inflammatory cells and inhibited the expression of inflammatory cytokines in AD-like skin lesions; (iii) CAPCS reduced plasma levels of inflammatory cytokines; and (iv) CAPCS inhibited TLR2 and TLR4 protein expression in skin lesions. Topical application of CAPCS exhibits a therapeutic effect on AD by inhibiting inflammatory immune responses via recruiting helper T cells and engaging the TLR2 and TLR4 signaling pathways. Therefore, CAPCS may be useful for the treatment of AD.https://www.mdpi.com/1422-0067/23/19/11371atopic dermatitiscalcium-based antimicrobial peptides compoundsBALB/c micecytokines |
| spellingShingle | Qingfeng Liu Mengmeng Li Na Wang Chun He Xian Jiang Jingyi Li Calcium-Based Antimicrobial Peptide Compounds Attenuate DNFB-Induced Atopic Dermatitis-Like Skin Lesions via Th-Cells in BALB/c Mice International Journal of Molecular Sciences atopic dermatitis calcium-based antimicrobial peptides compounds BALB/c mice cytokines |
| title | Calcium-Based Antimicrobial Peptide Compounds Attenuate DNFB-Induced Atopic Dermatitis-Like Skin Lesions via Th-Cells in BALB/c Mice |
| title_full | Calcium-Based Antimicrobial Peptide Compounds Attenuate DNFB-Induced Atopic Dermatitis-Like Skin Lesions via Th-Cells in BALB/c Mice |
| title_fullStr | Calcium-Based Antimicrobial Peptide Compounds Attenuate DNFB-Induced Atopic Dermatitis-Like Skin Lesions via Th-Cells in BALB/c Mice |
| title_full_unstemmed | Calcium-Based Antimicrobial Peptide Compounds Attenuate DNFB-Induced Atopic Dermatitis-Like Skin Lesions via Th-Cells in BALB/c Mice |
| title_short | Calcium-Based Antimicrobial Peptide Compounds Attenuate DNFB-Induced Atopic Dermatitis-Like Skin Lesions via Th-Cells in BALB/c Mice |
| title_sort | calcium based antimicrobial peptide compounds attenuate dnfb induced atopic dermatitis like skin lesions via th cells in balb c mice |
| topic | atopic dermatitis calcium-based antimicrobial peptides compounds BALB/c mice cytokines |
| url | https://www.mdpi.com/1422-0067/23/19/11371 |
| work_keys_str_mv | AT qingfengliu calciumbasedantimicrobialpeptidecompoundsattenuatednfbinducedatopicdermatitislikeskinlesionsviathcellsinbalbcmice AT mengmengli calciumbasedantimicrobialpeptidecompoundsattenuatednfbinducedatopicdermatitislikeskinlesionsviathcellsinbalbcmice AT nawang calciumbasedantimicrobialpeptidecompoundsattenuatednfbinducedatopicdermatitislikeskinlesionsviathcellsinbalbcmice AT chunhe calciumbasedantimicrobialpeptidecompoundsattenuatednfbinducedatopicdermatitislikeskinlesionsviathcellsinbalbcmice AT xianjiang calciumbasedantimicrobialpeptidecompoundsattenuatednfbinducedatopicdermatitislikeskinlesionsviathcellsinbalbcmice AT jingyili calciumbasedantimicrobialpeptidecompoundsattenuatednfbinducedatopicdermatitislikeskinlesionsviathcellsinbalbcmice |