The isolation, analytical characterization by HPLC-UV and NMR spectroscopy, cytotoxic and antioxidant activities of baeomycesic acid from Thamnolia vermicularis var. subuliformis
The aim of this work was the analytical characterization of the β-orcinol depside, baeomycesic acid in lichens extracts. The extract of Thamnolia vermicularis var. subuliformis was analyzed by the two different methods, namely HPLC-UV and 1H NMR analysis. The results showed that baeomycesic acid...
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Association of Chemical Engineers of Serbia
2011-01-01
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| Series: | Hemijska Industrija |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0367-598X/2011/0367-598X1100035M.pdf |
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| author | Manojlović Nedeljko T. Vasiljević Perica J. Nikolić Dragan Bogdanović-Dušanović Gordana Marković Zoran S. Najman Stevo |
| author_facet | Manojlović Nedeljko T. Vasiljević Perica J. Nikolić Dragan Bogdanović-Dušanović Gordana Marković Zoran S. Najman Stevo |
| author_sort | Manojlović Nedeljko T. |
| collection | DOAJ |
| description | The aim of this work was the analytical characterization of the β-orcinol depside, baeomycesic acid in lichens extracts. The extract of Thamnolia vermicularis var. subuliformis was analyzed by the two different methods, namely HPLC-UV and 1H NMR analysis. The results showed that baeomycesic acid was the most abundant depside in the lichens. These results could be of use for rapid identification of this metabolite in other lichen species. Besides baeomycesic acid, three depsides and one monocyclic phenolic compound were isolated from the lichen extract on the chromatographic column. The structure of baeomycesic acid was confirmed by HPLC-UV and spectroscopic methods. In addition, antioxidant and cytotoxic activities of baeomycesic acid were determined. The result of the testing showed that baeomycesic acid exhibited a moderate radical scavenging activity (IC50 = 602.10 ± 0.54 μg/mL) and good cytotoxic activity. This is the first report of detailed analytical characterization, isolation, as well as antioxidant and cytotoxic activities of baeomycesic acid from Thamnolia vermicularis. These results may be helpful in future industrial production of herbal medicines that include this important natural product. |
| first_indexed | 2024-12-14T14:28:30Z |
| format | Article |
| id | doaj.art-4febc495898a4f068daca4689e9aad38 |
| institution | Directory Open Access Journal |
| issn | 0367-598X |
| language | English |
| last_indexed | 2024-12-14T14:28:30Z |
| publishDate | 2011-01-01 |
| publisher | Association of Chemical Engineers of Serbia |
| record_format | Article |
| series | Hemijska Industrija |
| spelling | doaj.art-4febc495898a4f068daca4689e9aad382022-12-21T22:57:53ZengAssociation of Chemical Engineers of SerbiaHemijska Industrija0367-598X2011-01-0165559159810.2298/HEMIND110414035MThe isolation, analytical characterization by HPLC-UV and NMR spectroscopy, cytotoxic and antioxidant activities of baeomycesic acid from Thamnolia vermicularis var. subuliformisManojlović Nedeljko T.Vasiljević Perica J.Nikolić DraganBogdanović-Dušanović GordanaMarković Zoran S.Najman StevoThe aim of this work was the analytical characterization of the β-orcinol depside, baeomycesic acid in lichens extracts. The extract of Thamnolia vermicularis var. subuliformis was analyzed by the two different methods, namely HPLC-UV and 1H NMR analysis. The results showed that baeomycesic acid was the most abundant depside in the lichens. These results could be of use for rapid identification of this metabolite in other lichen species. Besides baeomycesic acid, three depsides and one monocyclic phenolic compound were isolated from the lichen extract on the chromatographic column. The structure of baeomycesic acid was confirmed by HPLC-UV and spectroscopic methods. In addition, antioxidant and cytotoxic activities of baeomycesic acid were determined. The result of the testing showed that baeomycesic acid exhibited a moderate radical scavenging activity (IC50 = 602.10 ± 0.54 μg/mL) and good cytotoxic activity. This is the first report of detailed analytical characterization, isolation, as well as antioxidant and cytotoxic activities of baeomycesic acid from Thamnolia vermicularis. These results may be helpful in future industrial production of herbal medicines that include this important natural product.http://www.doiserbia.nb.rs/img/doi/0367-598X/2011/0367-598X1100035M.pdfbaeomycesic acidNMRHPLCantioxidant activitycytotoxic activityHeLa cells |
| spellingShingle | Manojlović Nedeljko T. Vasiljević Perica J. Nikolić Dragan Bogdanović-Dušanović Gordana Marković Zoran S. Najman Stevo The isolation, analytical characterization by HPLC-UV and NMR spectroscopy, cytotoxic and antioxidant activities of baeomycesic acid from Thamnolia vermicularis var. subuliformis Hemijska Industrija baeomycesic acid NMR HPLC antioxidant activity cytotoxic activity HeLa cells |
| title | The isolation, analytical characterization by HPLC-UV and NMR spectroscopy, cytotoxic and antioxidant activities of baeomycesic acid from Thamnolia vermicularis var. subuliformis |
| title_full | The isolation, analytical characterization by HPLC-UV and NMR spectroscopy, cytotoxic and antioxidant activities of baeomycesic acid from Thamnolia vermicularis var. subuliformis |
| title_fullStr | The isolation, analytical characterization by HPLC-UV and NMR spectroscopy, cytotoxic and antioxidant activities of baeomycesic acid from Thamnolia vermicularis var. subuliformis |
| title_full_unstemmed | The isolation, analytical characterization by HPLC-UV and NMR spectroscopy, cytotoxic and antioxidant activities of baeomycesic acid from Thamnolia vermicularis var. subuliformis |
| title_short | The isolation, analytical characterization by HPLC-UV and NMR spectroscopy, cytotoxic and antioxidant activities of baeomycesic acid from Thamnolia vermicularis var. subuliformis |
| title_sort | isolation analytical characterization by hplc uv and nmr spectroscopy cytotoxic and antioxidant activities of baeomycesic acid from thamnolia vermicularis var subuliformis |
| topic | baeomycesic acid NMR HPLC antioxidant activity cytotoxic activity HeLa cells |
| url | http://www.doiserbia.nb.rs/img/doi/0367-598X/2011/0367-598X1100035M.pdf |
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