Comparison of gut microflora of donkeys in high and low altitude areas
Donkeys’ gut microbe is critical for their health and adaptation to the environment. Little research has been conducted on the donkey gut microbiome compared with other domestic animals. The Tibetan Plateau is an extreme environment. In this study, 6 Qinghai donkeys (QH) from the Tibetan Plateau and...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.964799/full |
| _version_ | 1811258717384998912 |
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| author | Rong Guo Shuer Zhang Jianxing Chen Wei Shen Wei Shen Guoliang Zhang Guoliang Zhang Junjie Wang Junjie Wang Fali Zhang Fali Zhang Qingjie Pan Taifeng Xie Deqiang Ai Jianbao Dong Jiajia Suo Yujiang Sun Yujiang Sun Yujiang Sun Shuqin Liu Shuqin Liu |
| author_facet | Rong Guo Shuer Zhang Jianxing Chen Wei Shen Wei Shen Guoliang Zhang Guoliang Zhang Junjie Wang Junjie Wang Fali Zhang Fali Zhang Qingjie Pan Taifeng Xie Deqiang Ai Jianbao Dong Jiajia Suo Yujiang Sun Yujiang Sun Yujiang Sun Shuqin Liu Shuqin Liu |
| author_sort | Rong Guo |
| collection | DOAJ |
| description | Donkeys’ gut microbe is critical for their health and adaptation to the environment. Little research has been conducted on the donkey gut microbiome compared with other domestic animals. The Tibetan Plateau is an extreme environment. In this study, 6 Qinghai donkeys (QH) from the Tibetan Plateau and 6 Dezhou donkeys (DZ) were investigated, and the contents of 4 parts—stomach, small intestine, cecum, and rectum—were collected. 16S rRNA sequencing and metagenomic sequencing were used to analyze the composition and diversity of gut microbial communities in donkeys. The results showed that the flora diversity and richness of the hindgut were significantly higher than those of the foregut (p < 0.01), with no sex differences, and the community structure and composition of the same or adjacent regions (stomach, small intestine, cecum, and rectum) were similar. Besides, the flora diversity and richness of QH on the Tibetan Plateau were significantly higher than those of DZ (p < 0.05). The major pathways associated with QH were signal transduction mechanisms and carbohydrate transport and metabolism, and Bacteroidales were the major contributors to these functions. Our study provides novel insights into the contribution of microbiomes to the adaptive evolution of donkeys. |
| first_indexed | 2024-04-12T18:18:47Z |
| format | Article |
| id | doaj.art-4fef17cb27574989a0dd44925d9507b8 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T18:18:47Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-4fef17cb27574989a0dd44925d9507b82022-12-22T03:21:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.964799964799Comparison of gut microflora of donkeys in high and low altitude areasRong Guo0Shuer Zhang1Jianxing Chen2Wei Shen3Wei Shen4Guoliang Zhang5Guoliang Zhang6Junjie Wang7Junjie Wang8Fali Zhang9Fali Zhang10Qingjie Pan11Taifeng Xie12Deqiang Ai13Jianbao Dong14Jiajia Suo15Yujiang Sun16Yujiang Sun17Yujiang Sun18Shuqin Liu19Shuqin Liu20College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, ChinaShandong Animal Husbandry General Station, Jinan, Shandong, ChinaCollege of Chemistry and Life Science, Chifeng University, Chifeng, Inner Mongolia, ChinaGene Bank of Equine Genetic Resources, Qingdao, Shandong, ChinaCollege of Life Sciences, Qingdao Agricultural University, Qingdao, Shandong, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, ChinaGene Bank of Equine Genetic Resources, Qingdao, Shandong, ChinaGene Bank of Equine Genetic Resources, Qingdao, Shandong, ChinaCollege of Life Sciences, Qingdao Agricultural University, Qingdao, Shandong, ChinaGene Bank of Equine Genetic Resources, Qingdao, Shandong, ChinaCollege of Life Sciences, Qingdao Agricultural University, Qingdao, Shandong, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, ChinaQinghai Sheep Breeding and Extension Service Center, Gangcha County, Haibei Prefecture, Qinghai, ChinaDepartment of Veterinary Medical Science, Shandong Vocational Animal Science and Veterinary College, Weifang, Shandong, ChinaDepartment of Veterinary Medical Science, Shandong Vocational Animal Science and Veterinary College, Weifang, Shandong, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, ChinaGene Bank of Equine Genetic Resources, Qingdao, Shandong, ChinaVocational College of Dongying, Dongying, Shandong, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, ChinaGene Bank of Equine Genetic Resources, Qingdao, Shandong, ChinaDonkeys’ gut microbe is critical for their health and adaptation to the environment. Little research has been conducted on the donkey gut microbiome compared with other domestic animals. The Tibetan Plateau is an extreme environment. In this study, 6 Qinghai donkeys (QH) from the Tibetan Plateau and 6 Dezhou donkeys (DZ) were investigated, and the contents of 4 parts—stomach, small intestine, cecum, and rectum—were collected. 16S rRNA sequencing and metagenomic sequencing were used to analyze the composition and diversity of gut microbial communities in donkeys. The results showed that the flora diversity and richness of the hindgut were significantly higher than those of the foregut (p < 0.01), with no sex differences, and the community structure and composition of the same or adjacent regions (stomach, small intestine, cecum, and rectum) were similar. Besides, the flora diversity and richness of QH on the Tibetan Plateau were significantly higher than those of DZ (p < 0.05). The major pathways associated with QH were signal transduction mechanisms and carbohydrate transport and metabolism, and Bacteroidales were the major contributors to these functions. Our study provides novel insights into the contribution of microbiomes to the adaptive evolution of donkeys.https://www.frontiersin.org/articles/10.3389/fmicb.2022.964799/fulldonkeygut microbesaltitude16S rRNAmetagenomic |
| spellingShingle | Rong Guo Shuer Zhang Jianxing Chen Wei Shen Wei Shen Guoliang Zhang Guoliang Zhang Junjie Wang Junjie Wang Fali Zhang Fali Zhang Qingjie Pan Taifeng Xie Deqiang Ai Jianbao Dong Jiajia Suo Yujiang Sun Yujiang Sun Yujiang Sun Shuqin Liu Shuqin Liu Comparison of gut microflora of donkeys in high and low altitude areas Frontiers in Microbiology donkey gut microbes altitude 16S rRNA metagenomic |
| title | Comparison of gut microflora of donkeys in high and low altitude areas |
| title_full | Comparison of gut microflora of donkeys in high and low altitude areas |
| title_fullStr | Comparison of gut microflora of donkeys in high and low altitude areas |
| title_full_unstemmed | Comparison of gut microflora of donkeys in high and low altitude areas |
| title_short | Comparison of gut microflora of donkeys in high and low altitude areas |
| title_sort | comparison of gut microflora of donkeys in high and low altitude areas |
| topic | donkey gut microbes altitude 16S rRNA metagenomic |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.964799/full |
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