Oral microbiota study of the patients after hospitalisation for COVID-19, considering selected dental indices and antibiotic therapy using the next generation sequencing method (NGS)
ABSTRACTBackground Poor oral hygiene and the increased incidence and severity of periodontitis may exacerbate SARS-CoV-2 infection. The aim was to evaluate the oral microbiota of 60 participants divided into groups: COVID-19 convalescents who received antibiotics during hospitalization (I), COVID-19...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2023.2264591 |
| _version_ | 1797449956476321792 |
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| author | Barbara Brzychczy- Sroka Katarzyna Talaga-Ćwiertnia Agnieszka Sroka-Oleksiak Artur Gurgul Elżbieta Zarzecka-Francica Wojciech Ostrowski Janusz Kąkol Joanna Zarzecka Monika Brzychczy-Włoch |
| author_facet | Barbara Brzychczy- Sroka Katarzyna Talaga-Ćwiertnia Agnieszka Sroka-Oleksiak Artur Gurgul Elżbieta Zarzecka-Francica Wojciech Ostrowski Janusz Kąkol Joanna Zarzecka Monika Brzychczy-Włoch |
| author_sort | Barbara Brzychczy- Sroka |
| collection | DOAJ |
| description | ABSTRACTBackground Poor oral hygiene and the increased incidence and severity of periodontitis may exacerbate SARS-CoV-2 infection. The aim was to evaluate the oral microbiota of 60 participants divided into groups: COVID-19 convalescents who received antibiotics during hospitalization (I), COVID-19 convalescents without antibiotic therapy (II) and healthy individuals (III).Materials and Methods Dental examination was conducted, and oral health status was evaluated using selected dental indexes. Clinical samples (saliva, dorsal swabs, supragingival and subgingival plaque) were collected and used for metagenomic library to the next-generation sequencing (NGS) preparation.Results Each of the clinical materials in particular groups of patients showed a statistically significant and quantitatively different bacterial composition. Patients from group I showed significantly worse oral health, reflected by higher average values of dental indexes and also a higher percentage of Veillonella, Tannerella, Capnocytophaga and Selenomonas genera in comparison to other groups. Additionally, a statistically significant decrease in the amount of Akkermansia type in both groups with COVID-19 was observed for all materials.Conclusions The primary factor affecting the composition of oral microbiota was not the SARS-CoV-2 infection itself, but the use of antibiotic therapy. The increased percentage of pro-inflammatory pathogens observed in COVID-19 patients underscores the importance of preventing periodontal disease and improving oral hygiene in the future. |
| first_indexed | 2024-03-09T14:33:40Z |
| format | Article |
| id | doaj.art-4ff37e134f6c470e809cc378e5ca6d4a |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-03-09T14:33:40Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-4ff37e134f6c470e809cc378e5ca6d4a2023-11-27T17:25:00ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972023-12-0115110.1080/20002297.2023.2264591Oral microbiota study of the patients after hospitalisation for COVID-19, considering selected dental indices and antibiotic therapy using the next generation sequencing method (NGS)Barbara Brzychczy- Sroka0Katarzyna Talaga-Ćwiertnia1Agnieszka Sroka-Oleksiak2Artur Gurgul3Elżbieta Zarzecka-Francica4Wojciech Ostrowski5Janusz Kąkol6Joanna Zarzecka7Monika Brzychczy-Włoch8Department of Conservative Dentistry with Endodontics, Institute of Dentistry, Jagiellonian University Medical College, Kraków, PolandDepartment of Molecular Medical Microbiology, Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Kraków, PolandDepartment of Molecular Medical Microbiology, Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Kraków, PolandCenter for Experimental and Innovative Medicine, The University of Agriculture in Kraków, Kraków, PolandDepartment of Prosthodontics and Orthodontics, Institute of Dentistry, Jagiellonian University Medical College, Kraków, PolandDepartment of Conservative Dentistry with Endodontics, Institute of Dentistry, Jagiellonian University Medical College, Kraków, PolandUniversity Hospital in Cracow, Kraków, PolandDepartment of Conservative Dentistry with Endodontics, Institute of Dentistry, Jagiellonian University Medical College, Kraków, PolandDepartment of Molecular Medical Microbiology, Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Kraków, PolandABSTRACTBackground Poor oral hygiene and the increased incidence and severity of periodontitis may exacerbate SARS-CoV-2 infection. The aim was to evaluate the oral microbiota of 60 participants divided into groups: COVID-19 convalescents who received antibiotics during hospitalization (I), COVID-19 convalescents without antibiotic therapy (II) and healthy individuals (III).Materials and Methods Dental examination was conducted, and oral health status was evaluated using selected dental indexes. Clinical samples (saliva, dorsal swabs, supragingival and subgingival plaque) were collected and used for metagenomic library to the next-generation sequencing (NGS) preparation.Results Each of the clinical materials in particular groups of patients showed a statistically significant and quantitatively different bacterial composition. Patients from group I showed significantly worse oral health, reflected by higher average values of dental indexes and also a higher percentage of Veillonella, Tannerella, Capnocytophaga and Selenomonas genera in comparison to other groups. Additionally, a statistically significant decrease in the amount of Akkermansia type in both groups with COVID-19 was observed for all materials.Conclusions The primary factor affecting the composition of oral microbiota was not the SARS-CoV-2 infection itself, but the use of antibiotic therapy. The increased percentage of pro-inflammatory pathogens observed in COVID-19 patients underscores the importance of preventing periodontal disease and improving oral hygiene in the future.https://www.tandfonline.com/doi/10.1080/20002297.2023.2264591COVID-19oral microbiotaantibiotic therapyoral healthnext-generation sequencing (NGS)supragingival plaque |
| spellingShingle | Barbara Brzychczy- Sroka Katarzyna Talaga-Ćwiertnia Agnieszka Sroka-Oleksiak Artur Gurgul Elżbieta Zarzecka-Francica Wojciech Ostrowski Janusz Kąkol Joanna Zarzecka Monika Brzychczy-Włoch Oral microbiota study of the patients after hospitalisation for COVID-19, considering selected dental indices and antibiotic therapy using the next generation sequencing method (NGS) Journal of Oral Microbiology COVID-19 oral microbiota antibiotic therapy oral health next-generation sequencing (NGS) supragingival plaque |
| title | Oral microbiota study of the patients after hospitalisation for COVID-19, considering selected dental indices and antibiotic therapy using the next generation sequencing method (NGS) |
| title_full | Oral microbiota study of the patients after hospitalisation for COVID-19, considering selected dental indices and antibiotic therapy using the next generation sequencing method (NGS) |
| title_fullStr | Oral microbiota study of the patients after hospitalisation for COVID-19, considering selected dental indices and antibiotic therapy using the next generation sequencing method (NGS) |
| title_full_unstemmed | Oral microbiota study of the patients after hospitalisation for COVID-19, considering selected dental indices and antibiotic therapy using the next generation sequencing method (NGS) |
| title_short | Oral microbiota study of the patients after hospitalisation for COVID-19, considering selected dental indices and antibiotic therapy using the next generation sequencing method (NGS) |
| title_sort | oral microbiota study of the patients after hospitalisation for covid 19 considering selected dental indices and antibiotic therapy using the next generation sequencing method ngs |
| topic | COVID-19 oral microbiota antibiotic therapy oral health next-generation sequencing (NGS) supragingival plaque |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2023.2264591 |
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