Anti-Fibrotic and Anti-Angiogenic Activities of <i>Osbeckia octandra</i> Leaf Extracts in Thioacetamide-Induced Experimental Liver Cirrhosis
Chronic liver inflammation has become a major global health concern. In the absence of clinical surrogate markers to diagnose inflammatory liver disease, the intervention with effective drugs in modern medicine tends to be late. In Sri Lanka, traditional medical practitioners prescribe herbal prepar...
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2021-08-01
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| author | Sudarma Bogahawaththa Suranga P. Kodithuwakku Kavindra K. Wijesundera Eranga H. Siriweera Lalith Jayasinghe Waruna L. Dissanayaka Jayanthe Rajapakse Chandana B. Herath Tadayuki Tsujita Missaka P. B. Wijayagunawardane |
| author_facet | Sudarma Bogahawaththa Suranga P. Kodithuwakku Kavindra K. Wijesundera Eranga H. Siriweera Lalith Jayasinghe Waruna L. Dissanayaka Jayanthe Rajapakse Chandana B. Herath Tadayuki Tsujita Missaka P. B. Wijayagunawardane |
| author_sort | Sudarma Bogahawaththa |
| collection | DOAJ |
| description | Chronic liver inflammation has become a major global health concern. In the absence of clinical surrogate markers to diagnose inflammatory liver disease, the intervention with effective drugs in modern medicine tends to be late. In Sri Lanka, traditional medical practitioners prescribe herbal preparations from <i>Osbeckia octandra</i> for the prevention and treatment of liver disorders. To test the efficacy of such treatments, we have administered thioacetamide (TAA) to male Wistar rats to induce chronic liver damage (disease control; DC) and examined how various leaf extracts: crude leaf suspension (CLS), boiled leaf extract (BLE), sonicated leaf extract (SLE), methanol leaf extract (MLE) and hexane leaf extract (HLE) of <i>O. octandra</i> ameliorate TAA-induced liver disease. The CLS, BLE and SLE treatments in cirrhotic rats significantly attenuated disease-related changes, such as liver weight and hepato-enzymes. The mRNA levels of <i>Tnf-α</i> were significantly decreased by 3.6, 10 and 3.9 times in CLS, BLE and SLE compared to DC. The same treatments resulted in significantly lower (19.5, 4.2 and 2.4 times) <i>α-Sma</i> levels compared to DC. In addition, <i>Tgf-β1</i> and <i>Vegf-R2</i> mRNA expressions were significantly lower with the treatments. Moreover, BLE expressed a strong anti-angiogenic effect. We conclude that CLS, BLE and SLE from <i>O. octandra</i> have potent hepatic anti-fibrotic effects in TAA-induced liver cirrhosis. |
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| language | English |
| last_indexed | 2024-03-10T08:33:05Z |
| publishDate | 2021-08-01 |
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| spelling | doaj.art-4ff4fc9633e74f788ee05bae7780fcab2023-11-22T08:52:40ZengMDPI AGMolecules1420-30492021-08-012616483610.3390/molecules26164836Anti-Fibrotic and Anti-Angiogenic Activities of <i>Osbeckia octandra</i> Leaf Extracts in Thioacetamide-Induced Experimental Liver CirrhosisSudarma Bogahawaththa0Suranga P. Kodithuwakku1Kavindra K. Wijesundera2Eranga H. Siriweera3Lalith Jayasinghe4Waruna L. Dissanayaka5Jayanthe Rajapakse6Chandana B. Herath7Tadayuki Tsujita8Missaka P. B. Wijayagunawardane9Department of Animal Science, Faculty of Agriculture, University of Peradeniya, Peradeniya 20400, Sri LankaDepartment of Animal Science, Faculty of Agriculture, University of Peradeniya, Peradeniya 20400, Sri LankaDepartment of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya 20400, Sri LankaDepartment of Pathology, Faculty of Medicine, University of Peradeniya, Peradeniya 20400, Sri LankaNational Institute of Fundamental Studies, Hanthana Road, Kandy 20000, Sri LankaApplied Oral Sciences & Community Dental Care, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, ChinaDepartment of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya 20400, Sri LankaDepartment of Medicine, Austin Health, Melbourne Medical School, The University of Melbourne, Heidelberg, VIC 3084, AustraliaThe United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, JapanDepartment of Animal Science, Faculty of Agriculture, University of Peradeniya, Peradeniya 20400, Sri LankaChronic liver inflammation has become a major global health concern. In the absence of clinical surrogate markers to diagnose inflammatory liver disease, the intervention with effective drugs in modern medicine tends to be late. In Sri Lanka, traditional medical practitioners prescribe herbal preparations from <i>Osbeckia octandra</i> for the prevention and treatment of liver disorders. To test the efficacy of such treatments, we have administered thioacetamide (TAA) to male Wistar rats to induce chronic liver damage (disease control; DC) and examined how various leaf extracts: crude leaf suspension (CLS), boiled leaf extract (BLE), sonicated leaf extract (SLE), methanol leaf extract (MLE) and hexane leaf extract (HLE) of <i>O. octandra</i> ameliorate TAA-induced liver disease. The CLS, BLE and SLE treatments in cirrhotic rats significantly attenuated disease-related changes, such as liver weight and hepato-enzymes. The mRNA levels of <i>Tnf-α</i> were significantly decreased by 3.6, 10 and 3.9 times in CLS, BLE and SLE compared to DC. The same treatments resulted in significantly lower (19.5, 4.2 and 2.4 times) <i>α-Sma</i> levels compared to DC. In addition, <i>Tgf-β1</i> and <i>Vegf-R2</i> mRNA expressions were significantly lower with the treatments. Moreover, BLE expressed a strong anti-angiogenic effect. We conclude that CLS, BLE and SLE from <i>O. octandra</i> have potent hepatic anti-fibrotic effects in TAA-induced liver cirrhosis.https://www.mdpi.com/1420-3049/26/16/4836hepatoprotectiveanti-angiogenic effect<i>Osbeckia octandra</i>Wistar ratsthioacetamidecirrhosis |
| spellingShingle | Sudarma Bogahawaththa Suranga P. Kodithuwakku Kavindra K. Wijesundera Eranga H. Siriweera Lalith Jayasinghe Waruna L. Dissanayaka Jayanthe Rajapakse Chandana B. Herath Tadayuki Tsujita Missaka P. B. Wijayagunawardane Anti-Fibrotic and Anti-Angiogenic Activities of <i>Osbeckia octandra</i> Leaf Extracts in Thioacetamide-Induced Experimental Liver Cirrhosis Molecules hepatoprotective anti-angiogenic effect <i>Osbeckia octandra</i> Wistar rats thioacetamide cirrhosis |
| title | Anti-Fibrotic and Anti-Angiogenic Activities of <i>Osbeckia octandra</i> Leaf Extracts in Thioacetamide-Induced Experimental Liver Cirrhosis |
| title_full | Anti-Fibrotic and Anti-Angiogenic Activities of <i>Osbeckia octandra</i> Leaf Extracts in Thioacetamide-Induced Experimental Liver Cirrhosis |
| title_fullStr | Anti-Fibrotic and Anti-Angiogenic Activities of <i>Osbeckia octandra</i> Leaf Extracts in Thioacetamide-Induced Experimental Liver Cirrhosis |
| title_full_unstemmed | Anti-Fibrotic and Anti-Angiogenic Activities of <i>Osbeckia octandra</i> Leaf Extracts in Thioacetamide-Induced Experimental Liver Cirrhosis |
| title_short | Anti-Fibrotic and Anti-Angiogenic Activities of <i>Osbeckia octandra</i> Leaf Extracts in Thioacetamide-Induced Experimental Liver Cirrhosis |
| title_sort | anti fibrotic and anti angiogenic activities of i osbeckia octandra i leaf extracts in thioacetamide induced experimental liver cirrhosis |
| topic | hepatoprotective anti-angiogenic effect <i>Osbeckia octandra</i> Wistar rats thioacetamide cirrhosis |
| url | https://www.mdpi.com/1420-3049/26/16/4836 |
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