Expansion of intestinal Prevotella copri correlates with enhanced susceptibility to arthritis
Rheumatoid arthritis (RA) is a prevalent systemic autoimmune disease, caused by a combination of genetic and environmental factors. Animal models suggest a role for intestinal bacteria in supporting the systemic immune response required for joint inflammation. Here we performed 16S sequencing on 114...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2013-11-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/01202 |
| _version_ | 1797997553782882304 |
|---|---|
| author | Jose U Scher Andrew Sczesnak Randy S Longman Nicola Segata Carles Ubeda Craig Bielski Tim Rostron Vincenzo Cerundolo Eric G Pamer Steven B Abramson Curtis Huttenhower Dan R Littman |
| author_facet | Jose U Scher Andrew Sczesnak Randy S Longman Nicola Segata Carles Ubeda Craig Bielski Tim Rostron Vincenzo Cerundolo Eric G Pamer Steven B Abramson Curtis Huttenhower Dan R Littman |
| author_sort | Jose U Scher |
| collection | DOAJ |
| description | Rheumatoid arthritis (RA) is a prevalent systemic autoimmune disease, caused by a combination of genetic and environmental factors. Animal models suggest a role for intestinal bacteria in supporting the systemic immune response required for joint inflammation. Here we performed 16S sequencing on 114 stool samples from rheumatoid arthritis patients and controls, and shotgun sequencing on a subset of 44 such samples. We identified the presence of Prevotella copri as strongly correlated with disease in new-onset untreated rheumatoid arthritis (NORA) patients. Increases in Prevotella abundance correlated with a reduction in Bacteroides and a loss of reportedly beneficial microbes in NORA subjects. We also identified unique Prevotella genes that correlated with disease. Further, colonization of mice revealed the ability of P. copri to dominate the intestinal microbiota and resulted in an increased sensitivity to chemically induced colitis. This work identifies a potential role for P. copri in the pathogenesis of RA. |
| first_indexed | 2024-04-11T10:34:29Z |
| format | Article |
| id | doaj.art-4ff853344f7f429ba805020254f4914b |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T10:34:29Z |
| publishDate | 2013-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-4ff853344f7f429ba805020254f4914b2022-12-22T04:29:20ZengeLife Sciences Publications LtdeLife2050-084X2013-11-01210.7554/eLife.01202Expansion of intestinal Prevotella copri correlates with enhanced susceptibility to arthritisJose U Scher0Andrew Sczesnak1Randy S Longman2Nicola Segata3Carles Ubeda4Craig Bielski5Tim Rostron6Vincenzo Cerundolo7Eric G Pamer8Steven B Abramson9Curtis Huttenhower10Dan R Littman11Department of Medicine, New York University School of Medicine and Hospital for Joint Diseases, New York, United StatesMolecular Pathogenesis Program, The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, United States; Graduate Program in Bioinformatics and Computational Biology, University of California, San Francisco, San Francisco, United StatesMolecular Pathogenesis Program, The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, United States; Jill Roberts IBD Center, Department of Medicine, Weill Cornell Medical College, New York, United StatesCentre for Integrative Biology, University of Trento, Trento, Italy; Department of Biostatistics, Harvard School of Public Health, Boston, United StatesImmunology Program, Infectious Diseases Service, and The Lucille Castori Center for Microbes, Inflammation, and Cancer, Memorial Sloan-Kettering Cancer Center, New York, United States; Centro Superior de Investigacion en Salud Publica, University of Valencia, Valencia, SpainDepartment of Biostatistics, Harvard School of Public Health, Boston, United StatesDepartment of Medicine, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United KingdomDepartment of Medicine, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United KingdomImmunology Program, Infectious Diseases Service, and The Lucille Castori Center for Microbes, Inflammation, and Cancer, Memorial Sloan-Kettering Cancer Center, New York, United StatesDepartment of Medicine, New York University School of Medicine and Hospital for Joint Diseases, New York, United StatesDepartment of Biostatistics, Harvard School of Public Health, Boston, United StatesMolecular Pathogenesis Program, The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, United States; Howard Hughes Medical Institute, New York University School of Medicine, New York, United StatesRheumatoid arthritis (RA) is a prevalent systemic autoimmune disease, caused by a combination of genetic and environmental factors. Animal models suggest a role for intestinal bacteria in supporting the systemic immune response required for joint inflammation. Here we performed 16S sequencing on 114 stool samples from rheumatoid arthritis patients and controls, and shotgun sequencing on a subset of 44 such samples. We identified the presence of Prevotella copri as strongly correlated with disease in new-onset untreated rheumatoid arthritis (NORA) patients. Increases in Prevotella abundance correlated with a reduction in Bacteroides and a loss of reportedly beneficial microbes in NORA subjects. We also identified unique Prevotella genes that correlated with disease. Further, colonization of mice revealed the ability of P. copri to dominate the intestinal microbiota and resulted in an increased sensitivity to chemically induced colitis. This work identifies a potential role for P. copri in the pathogenesis of RA.https://elifesciences.org/articles/01202microbiomeinflammationautoimmunitymetagenomicsrheumatoidarthritis |
| spellingShingle | Jose U Scher Andrew Sczesnak Randy S Longman Nicola Segata Carles Ubeda Craig Bielski Tim Rostron Vincenzo Cerundolo Eric G Pamer Steven B Abramson Curtis Huttenhower Dan R Littman Expansion of intestinal Prevotella copri correlates with enhanced susceptibility to arthritis eLife microbiome inflammation autoimmunity metagenomics rheumatoid arthritis |
| title | Expansion of intestinal Prevotella copri correlates with enhanced susceptibility to arthritis |
| title_full | Expansion of intestinal Prevotella copri correlates with enhanced susceptibility to arthritis |
| title_fullStr | Expansion of intestinal Prevotella copri correlates with enhanced susceptibility to arthritis |
| title_full_unstemmed | Expansion of intestinal Prevotella copri correlates with enhanced susceptibility to arthritis |
| title_short | Expansion of intestinal Prevotella copri correlates with enhanced susceptibility to arthritis |
| title_sort | expansion of intestinal prevotella copri correlates with enhanced susceptibility to arthritis |
| topic | microbiome inflammation autoimmunity metagenomics rheumatoid arthritis |
| url | https://elifesciences.org/articles/01202 |
| work_keys_str_mv | AT joseuscher expansionofintestinalprevotellacopricorrelateswithenhancedsusceptibilitytoarthritis AT andrewsczesnak expansionofintestinalprevotellacopricorrelateswithenhancedsusceptibilitytoarthritis AT randyslongman expansionofintestinalprevotellacopricorrelateswithenhancedsusceptibilitytoarthritis AT nicolasegata expansionofintestinalprevotellacopricorrelateswithenhancedsusceptibilitytoarthritis AT carlesubeda expansionofintestinalprevotellacopricorrelateswithenhancedsusceptibilitytoarthritis AT craigbielski expansionofintestinalprevotellacopricorrelateswithenhancedsusceptibilitytoarthritis AT timrostron expansionofintestinalprevotellacopricorrelateswithenhancedsusceptibilitytoarthritis AT vincenzocerundolo expansionofintestinalprevotellacopricorrelateswithenhancedsusceptibilitytoarthritis AT ericgpamer expansionofintestinalprevotellacopricorrelateswithenhancedsusceptibilitytoarthritis AT stevenbabramson expansionofintestinalprevotellacopricorrelateswithenhancedsusceptibilitytoarthritis AT curtishuttenhower expansionofintestinalprevotellacopricorrelateswithenhancedsusceptibilitytoarthritis AT danrlittman expansionofintestinalprevotellacopricorrelateswithenhancedsusceptibilitytoarthritis |