Effects of micro-porosity and local BMP-2 administration on bioresorption of β-TCP and new bone formation
Abstract Background It has been reported that the microporous structure of calcium phosphate (CaP) ceramics is important to osteoconduction. Bone morphogenetic protein-2 (BMP-2) has been shown to be a promising alternative to bone grafting and a therapeutic agent promoting bone regeneration when del...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2019-07-01
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| Series: | Biomaterials Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40824-019-0161-2 |
| _version_ | 1797282756881809408 |
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| author | Atsuhito Kakuta Takaaki Tanaka Masaaki Chazono Hirokazu Komaki Seiichiro Kitasato Naoya Inagaki Shoshi Akiyama Keishi Marumo |
| author_facet | Atsuhito Kakuta Takaaki Tanaka Masaaki Chazono Hirokazu Komaki Seiichiro Kitasato Naoya Inagaki Shoshi Akiyama Keishi Marumo |
| author_sort | Atsuhito Kakuta |
| collection | DOAJ |
| description | Abstract Background It has been reported that the microporous structure of calcium phosphate (CaP) ceramics is important to osteoconduction. Bone morphogenetic protein-2 (BMP-2) has been shown to be a promising alternative to bone grafting and a therapeutic agent promoting bone regeneration when delivered locally. The aim of this study was to evaluate the effects of micro-porosity within beta-tricalcium phosphate (β-TCP) cylinders and local BMP-2 administration on β-TCP resorption and new bone formation. Methods Bilateral cylindrical bone defects were created in rabbit distal femora, and the defects were filled with β-TCP. Rabbits were divided into 3 groups; defects were filled with a β-TCP cylinder with a total of approximately 60% porosity (Group A: 13.4% micro- and 46.9% macropore, Group B: 38.5% micro- and 20.3% macropore, Group C: the same micro- and macro-porosity as in group B supplemented with BMP-2). Rabbits were sacrificed 4, 8, 12, and 24 weeks postoperatively. Results The number of TRAP-positive cells and new bone formation in group B were significantly greater than those in group A at every period. The amount of residual β-TCP in group C was less than that in group B at all time periods, resulting in significantly more new bone formation in group C at 8 and 12 weeks. The number of TRAP-positive cells in group C was maximum at 4 weeks. Conclusions These results suggest that the amount of submicron microporous structure and local BMP-2 administration accelerated both osteoclastic resorption of β-TCP and new bone formation, probably through a coupling-like phenomenon between resorption and new bone formation. |
| first_indexed | 2024-03-07T17:18:02Z |
| format | Article |
| id | doaj.art-4ffcc24b9a554d8588b0a0724d62d540 |
| institution | Directory Open Access Journal |
| issn | 2055-7124 |
| language | English |
| last_indexed | 2024-03-07T17:18:02Z |
| publishDate | 2019-07-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Biomaterials Research |
| spelling | doaj.art-4ffcc24b9a554d8588b0a0724d62d5402024-03-02T21:35:25ZengAmerican Association for the Advancement of Science (AAAS)Biomaterials Research2055-71242019-07-012311910.1186/s40824-019-0161-2Effects of micro-porosity and local BMP-2 administration on bioresorption of β-TCP and new bone formationAtsuhito Kakuta0Takaaki Tanaka1Masaaki Chazono2Hirokazu Komaki3Seiichiro Kitasato4Naoya Inagaki5Shoshi Akiyama6Keishi Marumo7Department of Orthopaedic Surgery, Jikei University School of MedicineDepartment of Orthopaedic Surgery, Jikei University School of MedicineDepartment of Orthopaedic Surgery, Jikei University School of MedicineDepartment of Orthopaedic Surgery, Jikei University School of MedicineDepartment of Orthopaedic Surgery, Jikei University School of MedicineDepartment of Orthopaedic Surgery, Jikei University School of MedicineDepartment of Orthopaedic Surgery, Jikei University School of MedicineDepartment of Orthopaedic Surgery, Jikei University School of MedicineAbstract Background It has been reported that the microporous structure of calcium phosphate (CaP) ceramics is important to osteoconduction. Bone morphogenetic protein-2 (BMP-2) has been shown to be a promising alternative to bone grafting and a therapeutic agent promoting bone regeneration when delivered locally. The aim of this study was to evaluate the effects of micro-porosity within beta-tricalcium phosphate (β-TCP) cylinders and local BMP-2 administration on β-TCP resorption and new bone formation. Methods Bilateral cylindrical bone defects were created in rabbit distal femora, and the defects were filled with β-TCP. Rabbits were divided into 3 groups; defects were filled with a β-TCP cylinder with a total of approximately 60% porosity (Group A: 13.4% micro- and 46.9% macropore, Group B: 38.5% micro- and 20.3% macropore, Group C: the same micro- and macro-porosity as in group B supplemented with BMP-2). Rabbits were sacrificed 4, 8, 12, and 24 weeks postoperatively. Results The number of TRAP-positive cells and new bone formation in group B were significantly greater than those in group A at every period. The amount of residual β-TCP in group C was less than that in group B at all time periods, resulting in significantly more new bone formation in group C at 8 and 12 weeks. The number of TRAP-positive cells in group C was maximum at 4 weeks. Conclusions These results suggest that the amount of submicron microporous structure and local BMP-2 administration accelerated both osteoclastic resorption of β-TCP and new bone formation, probably through a coupling-like phenomenon between resorption and new bone formation.http://link.springer.com/article/10.1186/s40824-019-0161-2Beta-tricalcium phosphate (β-TCP)Microporous structureBone morphogenetic protein (BMP)Bone formationOsteoclasts |
| spellingShingle | Atsuhito Kakuta Takaaki Tanaka Masaaki Chazono Hirokazu Komaki Seiichiro Kitasato Naoya Inagaki Shoshi Akiyama Keishi Marumo Effects of micro-porosity and local BMP-2 administration on bioresorption of β-TCP and new bone formation Biomaterials Research Beta-tricalcium phosphate (β-TCP) Microporous structure Bone morphogenetic protein (BMP) Bone formation Osteoclasts |
| title | Effects of micro-porosity and local BMP-2 administration on bioresorption of β-TCP and new bone formation |
| title_full | Effects of micro-porosity and local BMP-2 administration on bioresorption of β-TCP and new bone formation |
| title_fullStr | Effects of micro-porosity and local BMP-2 administration on bioresorption of β-TCP and new bone formation |
| title_full_unstemmed | Effects of micro-porosity and local BMP-2 administration on bioresorption of β-TCP and new bone formation |
| title_short | Effects of micro-porosity and local BMP-2 administration on bioresorption of β-TCP and new bone formation |
| title_sort | effects of micro porosity and local bmp 2 administration on bioresorption of β tcp and new bone formation |
| topic | Beta-tricalcium phosphate (β-TCP) Microporous structure Bone morphogenetic protein (BMP) Bone formation Osteoclasts |
| url | http://link.springer.com/article/10.1186/s40824-019-0161-2 |
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