RETRACTED ARTICLE: Exosomal lncRNA HNF1A-AS1 affects cisplatin resistance in cervical cancer cells through regulating microRNA-34b/TUFT1 axis
Abstract Background There is growing evidence of the role of long non-coding RNAs (lncRNAs) in cervical cancer (CC). The objective was to discuss whether exosomal lncRNA HNF1A-AS1 impacted drug resistance in CC via binding to microRNA-34b (miR-34b) and regulating TUFT1 expression. Methods The expres...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-12-01
|
| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12935-019-1042-4 |
| _version_ | 1797273162105225216 |
|---|---|
| author | Xiaoqiong Luo Jingxi Wei Feng-lian Yang Xiao-xia Pang Feng Shi Yu-xia Wei Bi-yun Liao Jun-li Wang |
| author_facet | Xiaoqiong Luo Jingxi Wei Feng-lian Yang Xiao-xia Pang Feng Shi Yu-xia Wei Bi-yun Liao Jun-li Wang |
| author_sort | Xiaoqiong Luo |
| collection | DOAJ |
| description | Abstract Background There is growing evidence of the role of long non-coding RNAs (lncRNAs) in cervical cancer (CC). The objective was to discuss whether exosomal lncRNA HNF1A-AS1 impacted drug resistance in CC via binding to microRNA-34b (miR-34b) and regulating TUFT1 expression. Methods The expression of HNF1A-AS1 in normal cervical epithelial cells, cisplatin (DDP)-sensitive cell line (HeLa/S) and DDP-resistant cell line (HeLa/DDP) cells were detected. HeLa/S and HeLa/DDP cells were interfered with HNF1A-AS1 to determine IC50, proliferation, colony formation and apoptosis of CC cells. The exosomes were isolated and identified. Subcellular localization of HNF1A-AS1, expression of miR-34b and TUFT1 in receptor cells were also verified. The binding site between HNF1A-AS1 and miR-34b, together with miR-34b and TUFT1 were confirmed. Tumorigenic ability of cells in nude mice was also detected. Results HNF1A-AS1 was upregulated in DDP-resistant cell line HeLa/DDP. Silencing HNF1A-AS1 suppressed CC cell proliferation and promoted its apoptosis. HNF1A-AS1 was found to act as a competing endogenous RNA (ceRNA) of miR-34b to promote the expression of TUFT1. Exosomes shuttled HNF1A-AS1 promoted the proliferation and drug resistance of CC cells and inhibited their apoptosis by upregulating the expression of TUFT1 and downregulating miR-34b. Furthermore, suppressed exosomal HNF1A-AS1 in combination with DDP inhibited tumor growth in nude mice. Conclusion Our study provides evidence that CC-secreted exosomes carrying HNF1A-AS1 as a ceRNA of miR-34b to promote the expression of TUFT1, thereby promoting the DDP resistance in CC cells. |
| first_indexed | 2024-03-07T14:39:37Z |
| format | Article |
| id | doaj.art-4ffd63ca867144579828d571f5bddb05 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-03-07T14:39:37Z |
| publishDate | 2019-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-4ffd63ca867144579828d571f5bddb052024-03-05T20:26:00ZengBMCCancer Cell International1475-28672019-12-0119111210.1186/s12935-019-1042-4RETRACTED ARTICLE: Exosomal lncRNA HNF1A-AS1 affects cisplatin resistance in cervical cancer cells through regulating microRNA-34b/TUFT1 axisXiaoqiong Luo0Jingxi Wei1Feng-lian Yang2Xiao-xia Pang3Feng Shi4Yu-xia Wei5Bi-yun Liao6Jun-li Wang7Center of Reproductive Medicine, Affiliated Hospital of Youjiang Medical College for NationalitiesCenter of Reproductive Medicine, Affiliated Hospital of Youjiang Medical College for NationalitiesYoujiang Medical College for NationalitiesYoujiang Medical College for NationalitiesYoujiang Medical College for NationalitiesCenter of Reproductive Medicine, Affiliated Hospital of Youjiang Medical College for NationalitiesCenter of Reproductive Medicine, Affiliated Hospital of Youjiang Medical College for NationalitiesCenter of Reproductive Medicine, Affiliated Hospital of Youjiang Medical College for NationalitiesAbstract Background There is growing evidence of the role of long non-coding RNAs (lncRNAs) in cervical cancer (CC). The objective was to discuss whether exosomal lncRNA HNF1A-AS1 impacted drug resistance in CC via binding to microRNA-34b (miR-34b) and regulating TUFT1 expression. Methods The expression of HNF1A-AS1 in normal cervical epithelial cells, cisplatin (DDP)-sensitive cell line (HeLa/S) and DDP-resistant cell line (HeLa/DDP) cells were detected. HeLa/S and HeLa/DDP cells were interfered with HNF1A-AS1 to determine IC50, proliferation, colony formation and apoptosis of CC cells. The exosomes were isolated and identified. Subcellular localization of HNF1A-AS1, expression of miR-34b and TUFT1 in receptor cells were also verified. The binding site between HNF1A-AS1 and miR-34b, together with miR-34b and TUFT1 were confirmed. Tumorigenic ability of cells in nude mice was also detected. Results HNF1A-AS1 was upregulated in DDP-resistant cell line HeLa/DDP. Silencing HNF1A-AS1 suppressed CC cell proliferation and promoted its apoptosis. HNF1A-AS1 was found to act as a competing endogenous RNA (ceRNA) of miR-34b to promote the expression of TUFT1. Exosomes shuttled HNF1A-AS1 promoted the proliferation and drug resistance of CC cells and inhibited their apoptosis by upregulating the expression of TUFT1 and downregulating miR-34b. Furthermore, suppressed exosomal HNF1A-AS1 in combination with DDP inhibited tumor growth in nude mice. Conclusion Our study provides evidence that CC-secreted exosomes carrying HNF1A-AS1 as a ceRNA of miR-34b to promote the expression of TUFT1, thereby promoting the DDP resistance in CC cells.https://doi.org/10.1186/s12935-019-1042-4Cervical cancerExosomesLncRNA HNF1A-AS1microRNA-34bTUFT1ceRNA |
| spellingShingle | Xiaoqiong Luo Jingxi Wei Feng-lian Yang Xiao-xia Pang Feng Shi Yu-xia Wei Bi-yun Liao Jun-li Wang RETRACTED ARTICLE: Exosomal lncRNA HNF1A-AS1 affects cisplatin resistance in cervical cancer cells through regulating microRNA-34b/TUFT1 axis Cancer Cell International Cervical cancer Exosomes LncRNA HNF1A-AS1 microRNA-34b TUFT1 ceRNA |
| title | RETRACTED ARTICLE: Exosomal lncRNA HNF1A-AS1 affects cisplatin resistance in cervical cancer cells through regulating microRNA-34b/TUFT1 axis |
| title_full | RETRACTED ARTICLE: Exosomal lncRNA HNF1A-AS1 affects cisplatin resistance in cervical cancer cells through regulating microRNA-34b/TUFT1 axis |
| title_fullStr | RETRACTED ARTICLE: Exosomal lncRNA HNF1A-AS1 affects cisplatin resistance in cervical cancer cells through regulating microRNA-34b/TUFT1 axis |
| title_full_unstemmed | RETRACTED ARTICLE: Exosomal lncRNA HNF1A-AS1 affects cisplatin resistance in cervical cancer cells through regulating microRNA-34b/TUFT1 axis |
| title_short | RETRACTED ARTICLE: Exosomal lncRNA HNF1A-AS1 affects cisplatin resistance in cervical cancer cells through regulating microRNA-34b/TUFT1 axis |
| title_sort | retracted article exosomal lncrna hnf1a as1 affects cisplatin resistance in cervical cancer cells through regulating microrna 34b tuft1 axis |
| topic | Cervical cancer Exosomes LncRNA HNF1A-AS1 microRNA-34b TUFT1 ceRNA |
| url | https://doi.org/10.1186/s12935-019-1042-4 |
| work_keys_str_mv | AT xiaoqiongluo retractedarticleexosomallncrnahnf1aas1affectscisplatinresistanceincervicalcancercellsthroughregulatingmicrorna34btuft1axis AT jingxiwei retractedarticleexosomallncrnahnf1aas1affectscisplatinresistanceincervicalcancercellsthroughregulatingmicrorna34btuft1axis AT fenglianyang retractedarticleexosomallncrnahnf1aas1affectscisplatinresistanceincervicalcancercellsthroughregulatingmicrorna34btuft1axis AT xiaoxiapang retractedarticleexosomallncrnahnf1aas1affectscisplatinresistanceincervicalcancercellsthroughregulatingmicrorna34btuft1axis AT fengshi retractedarticleexosomallncrnahnf1aas1affectscisplatinresistanceincervicalcancercellsthroughregulatingmicrorna34btuft1axis AT yuxiawei retractedarticleexosomallncrnahnf1aas1affectscisplatinresistanceincervicalcancercellsthroughregulatingmicrorna34btuft1axis AT biyunliao retractedarticleexosomallncrnahnf1aas1affectscisplatinresistanceincervicalcancercellsthroughregulatingmicrorna34btuft1axis AT junliwang retractedarticleexosomallncrnahnf1aas1affectscisplatinresistanceincervicalcancercellsthroughregulatingmicrorna34btuft1axis |