Biological activities of leaf extracts from selected Kalanchoe species and their relationship with bufadienolides content
Context Kalanchoe species (Crassulaceae) are widely used in traditional medicine as remedies in infectious diseases and cancer treatment. Objective Cytotoxic and antimicrobial activities of Kalanchoe daigremontiana Raym.-Hamet & H. Perrier, K. pinnata (Lam.) Pers., and K. blossfeldiana Poelln. e...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13880209.2020.1795208 |
| _version_ | 1818572579144728576 |
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| author | Justyna Stefanowicz-Hajduk Anna Hering Magdalena Gucwa Rafał Hałasa Agata Soluch Mariusz Kowalczyk Anna Stochmal Renata Ochocka |
| author_facet | Justyna Stefanowicz-Hajduk Anna Hering Magdalena Gucwa Rafał Hałasa Agata Soluch Mariusz Kowalczyk Anna Stochmal Renata Ochocka |
| author_sort | Justyna Stefanowicz-Hajduk |
| collection | DOAJ |
| description | Context Kalanchoe species (Crassulaceae) are widely used in traditional medicine as remedies in infectious diseases and cancer treatment. Objective Cytotoxic and antimicrobial activities of Kalanchoe daigremontiana Raym.-Hamet & H. Perrier, K. pinnata (Lam.) Pers., and K. blossfeldiana Poelln. extracts were determined. The relationship between biological activities and the extracts bufadienolides content was also investigated. Materials and methods Fresh leaves of Kalanchoe species were macerated with 95% ethanol or water. The quantitative analysis of bufadienolides in the extracts was carried out with mass spectrometry. Cytotoxicity tests were performed on human cancer cell lines – HeLa, SKOV-3, MCF-7, and A375 by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay and Real-Time Cell Analysis system. The microbiological study was done using a few bacteria strains (β-hemolytic Streptococcus, Corynebacterium diphtheriae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus hirae, Escherichia coli) and Candida albicans. Results The K. blossfeldiana ethanol extract and K. daigremontiana water extract exhibited the most potent cytotoxic activity (IC50 < 19 µg/mL for HeLa and SKOV-3 cells). The strongest antibacterial effects showed ethanol extract of K. blossfeldiana and K. pinnata (MIC values were 8.45, 8.45, 0.25 and <33.75 µg/mL for S. aureus, S. epidermidis, and E. hirae, respectively). The highest total amount of bufadienolides was in K. daigremontiana ethanol extract. In contrast, K. blossfeldiana ethanol extract did not show the presence of these compounds. Conclusions Kalanchoe blossfeldiana ethanol extract is a potential candidate for cancer and bacterial infection treatment. Additionally, the biological effects of Kalanchoe extracts are not dependent on the presence and amount of bufadienolides in the plant extracts. |
| first_indexed | 2024-12-14T18:59:01Z |
| format | Article |
| id | doaj.art-5002825970fb4b55ad8f85a3e123209e |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-14T18:59:01Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-5002825970fb4b55ad8f85a3e123209e2022-12-21T22:51:00ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162020-01-0158173274010.1080/13880209.2020.17952081795208Biological activities of leaf extracts from selected Kalanchoe species and their relationship with bufadienolides contentJustyna Stefanowicz-Hajduk0Anna Hering1Magdalena Gucwa2Rafał Hałasa3Agata Soluch4Mariusz Kowalczyk5Anna Stochmal6Renata Ochocka7Department of Biology and Pharmaceutical Botany, Medical University of GdańskDepartment of Biology and Pharmaceutical Botany, Medical University of GdańskDepartment of Biology and Pharmaceutical Botany, Medical University of GdańskDepartment of Pharmaceutical Microbiology, Medical University of GdańskDepartment of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research InstituteDepartment of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research InstituteDepartment of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research InstituteDepartment of Biology and Pharmaceutical Botany, Medical University of GdańskContext Kalanchoe species (Crassulaceae) are widely used in traditional medicine as remedies in infectious diseases and cancer treatment. Objective Cytotoxic and antimicrobial activities of Kalanchoe daigremontiana Raym.-Hamet & H. Perrier, K. pinnata (Lam.) Pers., and K. blossfeldiana Poelln. extracts were determined. The relationship between biological activities and the extracts bufadienolides content was also investigated. Materials and methods Fresh leaves of Kalanchoe species were macerated with 95% ethanol or water. The quantitative analysis of bufadienolides in the extracts was carried out with mass spectrometry. Cytotoxicity tests were performed on human cancer cell lines – HeLa, SKOV-3, MCF-7, and A375 by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay and Real-Time Cell Analysis system. The microbiological study was done using a few bacteria strains (β-hemolytic Streptococcus, Corynebacterium diphtheriae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus hirae, Escherichia coli) and Candida albicans. Results The K. blossfeldiana ethanol extract and K. daigremontiana water extract exhibited the most potent cytotoxic activity (IC50 < 19 µg/mL for HeLa and SKOV-3 cells). The strongest antibacterial effects showed ethanol extract of K. blossfeldiana and K. pinnata (MIC values were 8.45, 8.45, 0.25 and <33.75 µg/mL for S. aureus, S. epidermidis, and E. hirae, respectively). The highest total amount of bufadienolides was in K. daigremontiana ethanol extract. In contrast, K. blossfeldiana ethanol extract did not show the presence of these compounds. Conclusions Kalanchoe blossfeldiana ethanol extract is a potential candidate for cancer and bacterial infection treatment. Additionally, the biological effects of Kalanchoe extracts are not dependent on the presence and amount of bufadienolides in the plant extracts.http://dx.doi.org/10.1080/13880209.2020.1795208kalanchoe daigremontianakalanchoe pinnatakalanchoe blossfeldianartca systemcytotoxicityhuman cancer cellscandida albicans |
| spellingShingle | Justyna Stefanowicz-Hajduk Anna Hering Magdalena Gucwa Rafał Hałasa Agata Soluch Mariusz Kowalczyk Anna Stochmal Renata Ochocka Biological activities of leaf extracts from selected Kalanchoe species and their relationship with bufadienolides content Pharmaceutical Biology kalanchoe daigremontiana kalanchoe pinnata kalanchoe blossfeldiana rtca system cytotoxicity human cancer cells candida albicans |
| title | Biological activities of leaf extracts from selected Kalanchoe species and their relationship with bufadienolides content |
| title_full | Biological activities of leaf extracts from selected Kalanchoe species and their relationship with bufadienolides content |
| title_fullStr | Biological activities of leaf extracts from selected Kalanchoe species and their relationship with bufadienolides content |
| title_full_unstemmed | Biological activities of leaf extracts from selected Kalanchoe species and their relationship with bufadienolides content |
| title_short | Biological activities of leaf extracts from selected Kalanchoe species and their relationship with bufadienolides content |
| title_sort | biological activities of leaf extracts from selected kalanchoe species and their relationship with bufadienolides content |
| topic | kalanchoe daigremontiana kalanchoe pinnata kalanchoe blossfeldiana rtca system cytotoxicity human cancer cells candida albicans |
| url | http://dx.doi.org/10.1080/13880209.2020.1795208 |
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