Effects of supplemental tannic acid on growth performance, gut health, microbiota, and fat accumulation and optimal dosages of tannic acid in broilers
This study was conducted to investigate the effects of different dosages of tannic acid (TA) on growth performance, nutrient digestibility, gut health, immune system, oxidative status, microbial composition, volatile fatty acids (VFA), bone mineral density, and fat digestion and accumulation in broi...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
|
| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.912797/full |
| _version_ | 1828119302528565248 |
|---|---|
| author | Janghan Choi Sudhir Yadav Jinquan Wang Benjamin J. Lorentz Jeferson M. Lourenco Todd R. Callaway Woo Kyun Kim |
| author_facet | Janghan Choi Sudhir Yadav Jinquan Wang Benjamin J. Lorentz Jeferson M. Lourenco Todd R. Callaway Woo Kyun Kim |
| author_sort | Janghan Choi |
| collection | DOAJ |
| description | This study was conducted to investigate the effects of different dosages of tannic acid (TA) on growth performance, nutrient digestibility, gut health, immune system, oxidative status, microbial composition, volatile fatty acids (VFA), bone mineral density, and fat digestion and accumulation in broilers and to find optimal dosages of TA for efficient growth and gut health in broilers. A total of 320 male Cobb500 broilers were randomly distributed to 4 treatments with 8 replicates including 1) tannic acid 0 (TA0): basal diet without TA; 2) tannic acid 0.5 (TA0.5): basal diet with 0.5 g/kg TA; 3) tannic acid 1.5 (TA1.5); and 4) tannic acid 2.5 (TA2.5). Supplemental TA at levels greater than 972 mg/kg tended to reduce BW on D 21 (p = 0.05). The TA2.5 had significantly lower apparent ileal digestibility (AID) of crude protein compared to the TA0 group. The AID of ether extract tended to be reduced by TA at levels greater than 525 mg/kg (p = 0.08). The jejunal lipase activities tended to be reduced by TA at levels less than 595.3 mg/kg (p = 0.09). TA linearly decreased goblet cell density in the crypts of the jejunum (p < 0.05) and reduced mRNA expression of mucin two at levels less than 784.9 mg/kg and zonula occludens two at levels less than 892.6 mg/kg (p < 0.05). The TA0.5 group had higher activities of liver superoxide dismutase compared to the TA0 group (p < 0.05). Bone mineral density and contents tended to be linearly decreased by TA (p = 0.05), and the ratio of lean to fat was linearly decreased (p < 0.01). Total cecal VFA production tended to be linearly reduced by TA at levels greater than 850.9 mg/kg (p = 0.07). Supplemental TA tended to increase the relative abundance of the phylum Bacteroidetes (p = 0.1) and decrease the relative abundance of the phylum Proteobacteria (p = 0.1). The relative abundance of the family Rikenellaceae was the lowest at 500 mg/kg TA, and the relative abundance of the family Bacillaceae was the highest at 1,045 mg/kg TA. Collectively, these results indicate that the optimum level of supplemental TA would range between 500 and 900 mg/kg; this range of TA supplementation would improve gut health without negatively affecting growth performance in broilers under antibiotic-free conditions. |
| first_indexed | 2024-04-11T13:46:07Z |
| format | Article |
| id | doaj.art-500717cd5bde421ea080afe8460333a5 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-11T13:46:07Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-500717cd5bde421ea080afe8460333a52022-12-22T04:21:01ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-09-011310.3389/fphys.2022.912797912797Effects of supplemental tannic acid on growth performance, gut health, microbiota, and fat accumulation and optimal dosages of tannic acid in broilersJanghan Choi0Sudhir Yadav1Jinquan Wang2Benjamin J. Lorentz3Jeferson M. Lourenco4Todd R. Callaway5Woo Kyun Kim6Department of Poultry Science, University of Georgia, Athens, GA, United StatesDepartment of Poultry Science, University of Georgia, Athens, GA, United StatesDepartment of Poultry Science, University of Georgia, Athens, GA, United StatesDepartment of Poultry Science, University of Georgia, Athens, GA, United StatesDepartment of Animal and Dairy Science, University of Georgia, Athens, GA, United StatesDepartment of Animal and Dairy Science, University of Georgia, Athens, GA, United StatesDepartment of Poultry Science, University of Georgia, Athens, GA, United StatesThis study was conducted to investigate the effects of different dosages of tannic acid (TA) on growth performance, nutrient digestibility, gut health, immune system, oxidative status, microbial composition, volatile fatty acids (VFA), bone mineral density, and fat digestion and accumulation in broilers and to find optimal dosages of TA for efficient growth and gut health in broilers. A total of 320 male Cobb500 broilers were randomly distributed to 4 treatments with 8 replicates including 1) tannic acid 0 (TA0): basal diet without TA; 2) tannic acid 0.5 (TA0.5): basal diet with 0.5 g/kg TA; 3) tannic acid 1.5 (TA1.5); and 4) tannic acid 2.5 (TA2.5). Supplemental TA at levels greater than 972 mg/kg tended to reduce BW on D 21 (p = 0.05). The TA2.5 had significantly lower apparent ileal digestibility (AID) of crude protein compared to the TA0 group. The AID of ether extract tended to be reduced by TA at levels greater than 525 mg/kg (p = 0.08). The jejunal lipase activities tended to be reduced by TA at levels less than 595.3 mg/kg (p = 0.09). TA linearly decreased goblet cell density in the crypts of the jejunum (p < 0.05) and reduced mRNA expression of mucin two at levels less than 784.9 mg/kg and zonula occludens two at levels less than 892.6 mg/kg (p < 0.05). The TA0.5 group had higher activities of liver superoxide dismutase compared to the TA0 group (p < 0.05). Bone mineral density and contents tended to be linearly decreased by TA (p = 0.05), and the ratio of lean to fat was linearly decreased (p < 0.01). Total cecal VFA production tended to be linearly reduced by TA at levels greater than 850.9 mg/kg (p = 0.07). Supplemental TA tended to increase the relative abundance of the phylum Bacteroidetes (p = 0.1) and decrease the relative abundance of the phylum Proteobacteria (p = 0.1). The relative abundance of the family Rikenellaceae was the lowest at 500 mg/kg TA, and the relative abundance of the family Bacillaceae was the highest at 1,045 mg/kg TA. Collectively, these results indicate that the optimum level of supplemental TA would range between 500 and 900 mg/kg; this range of TA supplementation would improve gut health without negatively affecting growth performance in broilers under antibiotic-free conditions.https://www.frontiersin.org/articles/10.3389/fphys.2022.912797/fulltannic acidgut healthapparent ileal digestibilityfat accumulationmicrobiotavolatile fatty acids |
| spellingShingle | Janghan Choi Sudhir Yadav Jinquan Wang Benjamin J. Lorentz Jeferson M. Lourenco Todd R. Callaway Woo Kyun Kim Effects of supplemental tannic acid on growth performance, gut health, microbiota, and fat accumulation and optimal dosages of tannic acid in broilers Frontiers in Physiology tannic acid gut health apparent ileal digestibility fat accumulation microbiota volatile fatty acids |
| title | Effects of supplemental tannic acid on growth performance, gut health, microbiota, and fat accumulation and optimal dosages of tannic acid in broilers |
| title_full | Effects of supplemental tannic acid on growth performance, gut health, microbiota, and fat accumulation and optimal dosages of tannic acid in broilers |
| title_fullStr | Effects of supplemental tannic acid on growth performance, gut health, microbiota, and fat accumulation and optimal dosages of tannic acid in broilers |
| title_full_unstemmed | Effects of supplemental tannic acid on growth performance, gut health, microbiota, and fat accumulation and optimal dosages of tannic acid in broilers |
| title_short | Effects of supplemental tannic acid on growth performance, gut health, microbiota, and fat accumulation and optimal dosages of tannic acid in broilers |
| title_sort | effects of supplemental tannic acid on growth performance gut health microbiota and fat accumulation and optimal dosages of tannic acid in broilers |
| topic | tannic acid gut health apparent ileal digestibility fat accumulation microbiota volatile fatty acids |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.912797/full |
| work_keys_str_mv | AT janghanchoi effectsofsupplementaltannicacidongrowthperformanceguthealthmicrobiotaandfataccumulationandoptimaldosagesoftannicacidinbroilers AT sudhiryadav effectsofsupplementaltannicacidongrowthperformanceguthealthmicrobiotaandfataccumulationandoptimaldosagesoftannicacidinbroilers AT jinquanwang effectsofsupplementaltannicacidongrowthperformanceguthealthmicrobiotaandfataccumulationandoptimaldosagesoftannicacidinbroilers AT benjaminjlorentz effectsofsupplementaltannicacidongrowthperformanceguthealthmicrobiotaandfataccumulationandoptimaldosagesoftannicacidinbroilers AT jefersonmlourenco effectsofsupplementaltannicacidongrowthperformanceguthealthmicrobiotaandfataccumulationandoptimaldosagesoftannicacidinbroilers AT toddrcallaway effectsofsupplementaltannicacidongrowthperformanceguthealthmicrobiotaandfataccumulationandoptimaldosagesoftannicacidinbroilers AT wookyunkim effectsofsupplementaltannicacidongrowthperformanceguthealthmicrobiotaandfataccumulationandoptimaldosagesoftannicacidinbroilers |