Antimicrobial Activities of Sponge-Derived Microorganisms from Coastal Waters of Central Vietnam
Bacteria associated with invertebrates are considered as good sources for biologically active compounds. Sponge-derived bacteria were screened for antimicrobial activities, the presence of the cluster genes of polyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs), and through a c...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
|
| Series: | Journal of Marine Science and Engineering |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-1312/8/8/594 |
| _version_ | 1797559396923867136 |
|---|---|
| author | Pham Thi Mien Dao Viet Ha Hoang Xuan Ben Bin Chen Lan Liu Phan Minh-Thu |
| author_facet | Pham Thi Mien Dao Viet Ha Hoang Xuan Ben Bin Chen Lan Liu Phan Minh-Thu |
| author_sort | Pham Thi Mien |
| collection | DOAJ |
| description | Bacteria associated with invertebrates are considered as good sources for biologically active compounds. Sponge-derived bacteria were screened for antimicrobial activities, the presence of the cluster genes of polyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs), and through a colony picking method. Crude extracts of broth cultures were tested for microbial inhibition. Eleven out of 25 isolated strains showed inhibition of at least one of eight tested indicator microorganisms. Antimicrobial activities were observed in the strains coded HM5, HM6, and HM9 with the presence of NRPS and PKSII genes, whereas the isolate HM21 held both NRPS and PKSII and inhibited only the growth of <i>Bacillus subtilis</i> by the well diffusion method and only inhibited <i>Serratia marcescens</i> by the colony picking method. Two isolates, HM5 and HM6, belonged to the species of <i>Bacillus</i>. Interestingly, the isolate HM9 was nearest to <i>Streptomyces mexicanus</i><sup>T</sup> NBRC100915 (GenBank accession number AB249966) with 94% sequence similarity. This potent strain HM9 could possibly be considered as a new species and a good source for bioactive compound discovery. Some isolates showed NRPS/PKS genes but did not exhibit antimicrobial activity. Thus, we suggested that both molecular and traditional methods should be conducted for the screening of antimicrobial producers. |
| first_indexed | 2024-03-10T17:44:46Z |
| format | Article |
| id | doaj.art-5007df60fc924ee889c93d2546e0dd7d |
| institution | Directory Open Access Journal |
| issn | 2077-1312 |
| language | English |
| last_indexed | 2024-03-10T17:44:46Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Marine Science and Engineering |
| spelling | doaj.art-5007df60fc924ee889c93d2546e0dd7d2023-11-20T09:32:52ZengMDPI AGJournal of Marine Science and Engineering2077-13122020-08-018859410.3390/jmse8080594Antimicrobial Activities of Sponge-Derived Microorganisms from Coastal Waters of Central VietnamPham Thi Mien0Dao Viet Ha1Hoang Xuan Ben2Bin Chen3Lan Liu4Phan Minh-Thu5Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang 650000, VietnamInstitute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang 650000, VietnamInstitute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang 650000, VietnamThe School of Marine Science, Sun Yat-Sen University, Guangzhou 510006, ChinaThe School of Marine Science, Sun Yat-Sen University, Guangzhou 510006, ChinaInstitute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang 650000, VietnamBacteria associated with invertebrates are considered as good sources for biologically active compounds. Sponge-derived bacteria were screened for antimicrobial activities, the presence of the cluster genes of polyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs), and through a colony picking method. Crude extracts of broth cultures were tested for microbial inhibition. Eleven out of 25 isolated strains showed inhibition of at least one of eight tested indicator microorganisms. Antimicrobial activities were observed in the strains coded HM5, HM6, and HM9 with the presence of NRPS and PKSII genes, whereas the isolate HM21 held both NRPS and PKSII and inhibited only the growth of <i>Bacillus subtilis</i> by the well diffusion method and only inhibited <i>Serratia marcescens</i> by the colony picking method. Two isolates, HM5 and HM6, belonged to the species of <i>Bacillus</i>. Interestingly, the isolate HM9 was nearest to <i>Streptomyces mexicanus</i><sup>T</sup> NBRC100915 (GenBank accession number AB249966) with 94% sequence similarity. This potent strain HM9 could possibly be considered as a new species and a good source for bioactive compound discovery. Some isolates showed NRPS/PKS genes but did not exhibit antimicrobial activity. Thus, we suggested that both molecular and traditional methods should be conducted for the screening of antimicrobial producers.https://www.mdpi.com/2077-1312/8/8/594sponge-associated bacteriaantimicrobial activities<i>Streptomyces</i><i>Bacillus</i> |
| spellingShingle | Pham Thi Mien Dao Viet Ha Hoang Xuan Ben Bin Chen Lan Liu Phan Minh-Thu Antimicrobial Activities of Sponge-Derived Microorganisms from Coastal Waters of Central Vietnam Journal of Marine Science and Engineering sponge-associated bacteria antimicrobial activities <i>Streptomyces</i> <i>Bacillus</i> |
| title | Antimicrobial Activities of Sponge-Derived Microorganisms from Coastal Waters of Central Vietnam |
| title_full | Antimicrobial Activities of Sponge-Derived Microorganisms from Coastal Waters of Central Vietnam |
| title_fullStr | Antimicrobial Activities of Sponge-Derived Microorganisms from Coastal Waters of Central Vietnam |
| title_full_unstemmed | Antimicrobial Activities of Sponge-Derived Microorganisms from Coastal Waters of Central Vietnam |
| title_short | Antimicrobial Activities of Sponge-Derived Microorganisms from Coastal Waters of Central Vietnam |
| title_sort | antimicrobial activities of sponge derived microorganisms from coastal waters of central vietnam |
| topic | sponge-associated bacteria antimicrobial activities <i>Streptomyces</i> <i>Bacillus</i> |
| url | https://www.mdpi.com/2077-1312/8/8/594 |
| work_keys_str_mv | AT phamthimien antimicrobialactivitiesofspongederivedmicroorganismsfromcoastalwatersofcentralvietnam AT daovietha antimicrobialactivitiesofspongederivedmicroorganismsfromcoastalwatersofcentralvietnam AT hoangxuanben antimicrobialactivitiesofspongederivedmicroorganismsfromcoastalwatersofcentralvietnam AT binchen antimicrobialactivitiesofspongederivedmicroorganismsfromcoastalwatersofcentralvietnam AT lanliu antimicrobialactivitiesofspongederivedmicroorganismsfromcoastalwatersofcentralvietnam AT phanminhthu antimicrobialactivitiesofspongederivedmicroorganismsfromcoastalwatersofcentralvietnam |