Prognostic marker CXCL5 in glioblastoma polyformis and its mechanism of immune invasion
Abstract Glioblastoma multiforme (GBM) is the most aggressive brain cancer with a poor prognosis. Therefore, the correlative molecular markers and molecular mechanisms should be explored to assess the occurrence and treatment of glioma.WB and qPCR assays were used to detect the expression of CXCL5 i...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Cancer |
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| Online Access: | https://doi.org/10.1186/s12885-023-11650-3 |
| _version_ | 1797274295584423936 |
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| author | Wangyang Yu Minfeng Zhou Huifang Niu Jinxiao Li Qiumeng Li Xiaoyun Xu Fengxia Liang Chen Rui |
| author_facet | Wangyang Yu Minfeng Zhou Huifang Niu Jinxiao Li Qiumeng Li Xiaoyun Xu Fengxia Liang Chen Rui |
| author_sort | Wangyang Yu |
| collection | DOAJ |
| description | Abstract Glioblastoma multiforme (GBM) is the most aggressive brain cancer with a poor prognosis. Therefore, the correlative molecular markers and molecular mechanisms should be explored to assess the occurrence and treatment of glioma.WB and qPCR assays were used to detect the expression of CXCL5 in human GBM tissues. The relationship between CXCL5 expression and clinicopathological features was evaluated using logistic regression analysis, Wilcoxon symbolic rank test, and Kruskal–Wallis test. Univariate, multivariate Cox regression and Kaplan–Meier methods were used to assess CXCL5 and other prognostic factors of GBM. Gene set enrichment analysis (GSEA) was used to identify pathways associated with CXCL5. The correlation between CXCL5 and tumor immunoinfiltration was investigated using single sample gene set enrichment analysis (ssGSEA) of TCGA data. Cell experiments and mouse subcutaneous transplanted tumor models were used to evaluate the role of CXCL5 in GBM. WB, qPCR, immunofluorescence, and immunohistochemical assays showed that CXCL5 expression was increased in human GBM tissues. Furthermore, high CXCL5 expression was closely related to poor disease-specific survival and overall survival of GBM patients. The ssGSEA suggested that CXCL5 is closely related to the cell cycle and immune response through PPAR signaling pathway. GSEA also showed that CXCL5 expression was positively correlated with macrophage cell infiltration level and negatively correlated with cytotoxic cell infiltration level. CXCL5 may be associated with the prognosis and immunoinfiltration of GBM. |
| first_indexed | 2024-03-07T14:56:21Z |
| format | Article |
| id | doaj.art-500964debbc14dc3b21f81d8ef023db0 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-03-07T14:56:21Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-500964debbc14dc3b21f81d8ef023db02024-03-05T19:23:00ZengBMCBMC Cancer1471-24072024-01-0124111510.1186/s12885-023-11650-3Prognostic marker CXCL5 in glioblastoma polyformis and its mechanism of immune invasionWangyang Yu0Minfeng Zhou1Huifang Niu2Jinxiao Li3Qiumeng Li4Xiaoyun Xu5Fengxia Liang6Chen Rui7Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and TechnologyUnion Hospital Affiliated to Tongji Medical College, Huazhong University of Science and TechnologyCollege of Food Science and Technology, Huazhong Agricultural UniversityUnion Hospital Affiliated to Tongji Medical College, Huazhong University of Science and TechnologyUnion Hospital Affiliated to Tongji Medical College, Huazhong University of Science and TechnologyCollege of Food Science and Technology, Huazhong Agricultural UniversitySchool of Acupuncture and Bone Injury, Hubei University of Chinese MedicineUnion Hospital Affiliated to Tongji Medical College, Huazhong University of Science and TechnologyAbstract Glioblastoma multiforme (GBM) is the most aggressive brain cancer with a poor prognosis. Therefore, the correlative molecular markers and molecular mechanisms should be explored to assess the occurrence and treatment of glioma.WB and qPCR assays were used to detect the expression of CXCL5 in human GBM tissues. The relationship between CXCL5 expression and clinicopathological features was evaluated using logistic regression analysis, Wilcoxon symbolic rank test, and Kruskal–Wallis test. Univariate, multivariate Cox regression and Kaplan–Meier methods were used to assess CXCL5 and other prognostic factors of GBM. Gene set enrichment analysis (GSEA) was used to identify pathways associated with CXCL5. The correlation between CXCL5 and tumor immunoinfiltration was investigated using single sample gene set enrichment analysis (ssGSEA) of TCGA data. Cell experiments and mouse subcutaneous transplanted tumor models were used to evaluate the role of CXCL5 in GBM. WB, qPCR, immunofluorescence, and immunohistochemical assays showed that CXCL5 expression was increased in human GBM tissues. Furthermore, high CXCL5 expression was closely related to poor disease-specific survival and overall survival of GBM patients. The ssGSEA suggested that CXCL5 is closely related to the cell cycle and immune response through PPAR signaling pathway. GSEA also showed that CXCL5 expression was positively correlated with macrophage cell infiltration level and negatively correlated with cytotoxic cell infiltration level. CXCL5 may be associated with the prognosis and immunoinfiltration of GBM.https://doi.org/10.1186/s12885-023-11650-3Glioblastoma multiforme (GBM)CXCL5Immune invasionBrain cancerImmunoinfiltration |
| spellingShingle | Wangyang Yu Minfeng Zhou Huifang Niu Jinxiao Li Qiumeng Li Xiaoyun Xu Fengxia Liang Chen Rui Prognostic marker CXCL5 in glioblastoma polyformis and its mechanism of immune invasion BMC Cancer Glioblastoma multiforme (GBM) CXCL5 Immune invasion Brain cancer Immunoinfiltration |
| title | Prognostic marker CXCL5 in glioblastoma polyformis and its mechanism of immune invasion |
| title_full | Prognostic marker CXCL5 in glioblastoma polyformis and its mechanism of immune invasion |
| title_fullStr | Prognostic marker CXCL5 in glioblastoma polyformis and its mechanism of immune invasion |
| title_full_unstemmed | Prognostic marker CXCL5 in glioblastoma polyformis and its mechanism of immune invasion |
| title_short | Prognostic marker CXCL5 in glioblastoma polyformis and its mechanism of immune invasion |
| title_sort | prognostic marker cxcl5 in glioblastoma polyformis and its mechanism of immune invasion |
| topic | Glioblastoma multiforme (GBM) CXCL5 Immune invasion Brain cancer Immunoinfiltration |
| url | https://doi.org/10.1186/s12885-023-11650-3 |
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