Comparison of healing of acute total tympanic membrane perforation between rats with and without excision of the mallear handle
Abstract Objective We compared the histological changes and hearing restoration during the healing of acute total tympanic membrane (TM) perforations between Sprague–Dawley (SD) rats with and without excision of the mallear handle. Materials and methods Bilateral, acute, and total TM perforations we...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | Laryngoscope Investigative Otolaryngology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/lio2.1175 |
| _version_ | 1797384802937077760 |
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| author | Zihan Lou Chunyan Li Dongzhen Yu Jingjing Wang Zhengnong Chen Shankai Yin |
| author_facet | Zihan Lou Chunyan Li Dongzhen Yu Jingjing Wang Zhengnong Chen Shankai Yin |
| author_sort | Zihan Lou |
| collection | DOAJ |
| description | Abstract Objective We compared the histological changes and hearing restoration during the healing of acute total tympanic membrane (TM) perforations between Sprague–Dawley (SD) rats with and without excision of the mallear handle. Materials and methods Bilateral, acute, and total TM perforations were created in 36 male SD rats. The mallear handle was preserved in the left ear (handle‐preserved ear [HPE]) and excised from the right ear (handle‐excised ear [HEE]). Endoscopical examination, auditory brainstem response (ABR) thresholds, histopathological, and scanning electron microscope (SEM) analysis were performed. Results Endoscopic photographs showed that all perforations in the 18 SD rats were closed. The mean closure times were 6.83 ± 0.85 and 8.50 ± 0.71 days in the HPE and HEE groups, respectively (p < .001). SEM images showed radial arrangement of fiber bundles in a single direction in HPEs, although normal arrangement was not achieved. In contrast, HEEs showed disorganized arrangement. At 1 month after perforation closure, the ABR thresholds at high frequencies were significantly higher in the HEE group than in the HPE group (p = .029 and p = .017 for 16 and 32 kHz, respectively). Additionally, the changes in ABR threshold were significantly different at high frequencies (p = .011 and p = .017 for 16 and 32 kHz, respectively) before and 1 month after perforation closure between the HPE and HEE groups, although the differences were not statistically significant at the remaining frequencies. Conclusion Although the malleus handle may not affect the closure of total perforation in SD rats, it contributes to accelerate the perforation closure by possible guide the migration of proliferative epithelial cell on the upper halves of the annulus. Additionally, resection of the malleus handle impairs high frequency hearing recovery following spontaneous closure of the TM. |
| first_indexed | 2024-03-08T21:44:55Z |
| format | Article |
| id | doaj.art-500a438c8e0d4287bae04d982611e638 |
| institution | Directory Open Access Journal |
| issn | 2378-8038 |
| language | English |
| last_indexed | 2024-03-08T21:44:55Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Laryngoscope Investigative Otolaryngology |
| spelling | doaj.art-500a438c8e0d4287bae04d982611e6382023-12-20T09:48:34ZengWileyLaryngoscope Investigative Otolaryngology2378-80382023-12-01861648165610.1002/lio2.1175Comparison of healing of acute total tympanic membrane perforation between rats with and without excision of the mallear handleZihan Lou0Chunyan Li1Dongzhen Yu2Jingjing Wang3Zhengnong Chen4Shankai Yin5Department of Otolaryngology‐Head and Neck Surgery Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai ChinaDepartment of Otolaryngology‐Head and Neck Surgery Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai ChinaDepartment of Otolaryngology‐Head and Neck Surgery Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai ChinaDepartment of Otolaryngology‐Head and Neck Surgery Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai ChinaDepartment of Otolaryngology‐Head and Neck Surgery Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai ChinaDepartment of Otolaryngology‐Head and Neck Surgery Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai ChinaAbstract Objective We compared the histological changes and hearing restoration during the healing of acute total tympanic membrane (TM) perforations between Sprague–Dawley (SD) rats with and without excision of the mallear handle. Materials and methods Bilateral, acute, and total TM perforations were created in 36 male SD rats. The mallear handle was preserved in the left ear (handle‐preserved ear [HPE]) and excised from the right ear (handle‐excised ear [HEE]). Endoscopical examination, auditory brainstem response (ABR) thresholds, histopathological, and scanning electron microscope (SEM) analysis were performed. Results Endoscopic photographs showed that all perforations in the 18 SD rats were closed. The mean closure times were 6.83 ± 0.85 and 8.50 ± 0.71 days in the HPE and HEE groups, respectively (p < .001). SEM images showed radial arrangement of fiber bundles in a single direction in HPEs, although normal arrangement was not achieved. In contrast, HEEs showed disorganized arrangement. At 1 month after perforation closure, the ABR thresholds at high frequencies were significantly higher in the HEE group than in the HPE group (p = .029 and p = .017 for 16 and 32 kHz, respectively). Additionally, the changes in ABR threshold were significantly different at high frequencies (p = .011 and p = .017 for 16 and 32 kHz, respectively) before and 1 month after perforation closure between the HPE and HEE groups, although the differences were not statistically significant at the remaining frequencies. Conclusion Although the malleus handle may not affect the closure of total perforation in SD rats, it contributes to accelerate the perforation closure by possible guide the migration of proliferative epithelial cell on the upper halves of the annulus. Additionally, resection of the malleus handle impairs high frequency hearing recovery following spontaneous closure of the TM.https://doi.org/10.1002/lio2.1175keratinocytemallear handlespontaneous healingtympanic membrane |
| spellingShingle | Zihan Lou Chunyan Li Dongzhen Yu Jingjing Wang Zhengnong Chen Shankai Yin Comparison of healing of acute total tympanic membrane perforation between rats with and without excision of the mallear handle Laryngoscope Investigative Otolaryngology keratinocyte mallear handle spontaneous healing tympanic membrane |
| title | Comparison of healing of acute total tympanic membrane perforation between rats with and without excision of the mallear handle |
| title_full | Comparison of healing of acute total tympanic membrane perforation between rats with and without excision of the mallear handle |
| title_fullStr | Comparison of healing of acute total tympanic membrane perforation between rats with and without excision of the mallear handle |
| title_full_unstemmed | Comparison of healing of acute total tympanic membrane perforation between rats with and without excision of the mallear handle |
| title_short | Comparison of healing of acute total tympanic membrane perforation between rats with and without excision of the mallear handle |
| title_sort | comparison of healing of acute total tympanic membrane perforation between rats with and without excision of the mallear handle |
| topic | keratinocyte mallear handle spontaneous healing tympanic membrane |
| url | https://doi.org/10.1002/lio2.1175 |
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