Cytokine profiling of samples positive for Chlamydia trachomatis and Human papillomavirus.
Persistent human papillomavirus (HPV) infection is closely associated with cervical carcinoma. Co-infection in the endocervical environment with other microorganisms, such as Chlamydia trachomatis, may increase the risk of HPV infection and neoplastic progression. While in some individuals, Chlamydi...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0279390 |
| _version_ | 1797847155684147200 |
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| author | Larissa Zatorre Almeida Lugo Marco Antonio Moreira Puga Camila Mareti Bonin Jacob Cacilda Tezelli Junqueira Padovani Mariana Calarge Nocetti Maisa Souza Tupiná Ana Flávia Silva Pina Jennifer Naed Martins de Freitas Alda Maria Teixeira Ferreira Carlos Eurico Dos Santos Fernandes Adriane Cristina Bovo Júlio César Possati Resende Inês Aparecida Tozetti |
| author_facet | Larissa Zatorre Almeida Lugo Marco Antonio Moreira Puga Camila Mareti Bonin Jacob Cacilda Tezelli Junqueira Padovani Mariana Calarge Nocetti Maisa Souza Tupiná Ana Flávia Silva Pina Jennifer Naed Martins de Freitas Alda Maria Teixeira Ferreira Carlos Eurico Dos Santos Fernandes Adriane Cristina Bovo Júlio César Possati Resende Inês Aparecida Tozetti |
| author_sort | Larissa Zatorre Almeida Lugo |
| collection | DOAJ |
| description | Persistent human papillomavirus (HPV) infection is closely associated with cervical carcinoma. Co-infection in the endocervical environment with other microorganisms, such as Chlamydia trachomatis, may increase the risk of HPV infection and neoplastic progression. While in some individuals, Chlamydia trachomatis infection is resolved with the activation of Th1/IFN-γ-mediated immune response, others develop a chronic infection marked by Th2-mediated immune response, resulting in intracellular persistence of the bacterium and increasing the risk of HPV infection. This work aimed to quantify cytokines of the Th1/Th2/Th17 profile in exfoliated cervix cells (ECC) and peripheral blood (PB) of patients positive for Chlamydia trachomatis DNA, patients positive for Papillomavirus DNA, and healthy patients. Cytokine levels were quantified by flow cytometry in ECC and PB samples from patients positive for C. trachomatis DNA (n = 18), patients positive for HPV DNA (n = 30), and healthy patients (n = 17) treated at the Hospital de Amor, Campo Grande-MS. After analysis, a higher concentration of IL-17, IL-6, and IL-4 (p <0.05) in ECC; INF-γ and IL-10 (p <0.05) in PB was found in samples from patients positive for C. trachomatis DNA compared to samples from healthy patients. When comparing samples from patients positive for HPV DNA, there was a higher concentration of cytokines IL-17, IL-10, IL-6, and IL-4 (p <0.05) in ECC and IL-4 and IL-2 (p <0.05) in PB of patients positive for C. trachomatis DNA. These results suggest that induction of Th2- and Th17 mediated immune response occurs in patients positive for C. trachomatis DNA, indicating chronic infection. Our results also demonstrate a high concentration of pro-inflammatory cytokines in ECC of patients positive for C. trachomatis DNA. |
| first_indexed | 2024-04-09T18:07:49Z |
| format | Article |
| id | doaj.art-500bb6e4fc134014b0baf35ce8caed08 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-09T18:07:49Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-500bb6e4fc134014b0baf35ce8caed082023-04-14T05:31:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01183e027939010.1371/journal.pone.0279390Cytokine profiling of samples positive for Chlamydia trachomatis and Human papillomavirus.Larissa Zatorre Almeida LugoMarco Antonio Moreira PugaCamila Mareti Bonin JacobCacilda Tezelli Junqueira PadovaniMariana Calarge NocettiMaisa Souza TupináAna Flávia Silva PinaJennifer Naed Martins de FreitasAlda Maria Teixeira FerreiraCarlos Eurico Dos Santos FernandesAdriane Cristina BovoJúlio César Possati ResendeInês Aparecida TozettiPersistent human papillomavirus (HPV) infection is closely associated with cervical carcinoma. Co-infection in the endocervical environment with other microorganisms, such as Chlamydia trachomatis, may increase the risk of HPV infection and neoplastic progression. While in some individuals, Chlamydia trachomatis infection is resolved with the activation of Th1/IFN-γ-mediated immune response, others develop a chronic infection marked by Th2-mediated immune response, resulting in intracellular persistence of the bacterium and increasing the risk of HPV infection. This work aimed to quantify cytokines of the Th1/Th2/Th17 profile in exfoliated cervix cells (ECC) and peripheral blood (PB) of patients positive for Chlamydia trachomatis DNA, patients positive for Papillomavirus DNA, and healthy patients. Cytokine levels were quantified by flow cytometry in ECC and PB samples from patients positive for C. trachomatis DNA (n = 18), patients positive for HPV DNA (n = 30), and healthy patients (n = 17) treated at the Hospital de Amor, Campo Grande-MS. After analysis, a higher concentration of IL-17, IL-6, and IL-4 (p <0.05) in ECC; INF-γ and IL-10 (p <0.05) in PB was found in samples from patients positive for C. trachomatis DNA compared to samples from healthy patients. When comparing samples from patients positive for HPV DNA, there was a higher concentration of cytokines IL-17, IL-10, IL-6, and IL-4 (p <0.05) in ECC and IL-4 and IL-2 (p <0.05) in PB of patients positive for C. trachomatis DNA. These results suggest that induction of Th2- and Th17 mediated immune response occurs in patients positive for C. trachomatis DNA, indicating chronic infection. Our results also demonstrate a high concentration of pro-inflammatory cytokines in ECC of patients positive for C. trachomatis DNA.https://doi.org/10.1371/journal.pone.0279390 |
| spellingShingle | Larissa Zatorre Almeida Lugo Marco Antonio Moreira Puga Camila Mareti Bonin Jacob Cacilda Tezelli Junqueira Padovani Mariana Calarge Nocetti Maisa Souza Tupiná Ana Flávia Silva Pina Jennifer Naed Martins de Freitas Alda Maria Teixeira Ferreira Carlos Eurico Dos Santos Fernandes Adriane Cristina Bovo Júlio César Possati Resende Inês Aparecida Tozetti Cytokine profiling of samples positive for Chlamydia trachomatis and Human papillomavirus. PLoS ONE |
| title | Cytokine profiling of samples positive for Chlamydia trachomatis and Human papillomavirus. |
| title_full | Cytokine profiling of samples positive for Chlamydia trachomatis and Human papillomavirus. |
| title_fullStr | Cytokine profiling of samples positive for Chlamydia trachomatis and Human papillomavirus. |
| title_full_unstemmed | Cytokine profiling of samples positive for Chlamydia trachomatis and Human papillomavirus. |
| title_short | Cytokine profiling of samples positive for Chlamydia trachomatis and Human papillomavirus. |
| title_sort | cytokine profiling of samples positive for chlamydia trachomatis and human papillomavirus |
| url | https://doi.org/10.1371/journal.pone.0279390 |
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