Inhibition of Estrogenic Response of Yeast Screen Assay by Exposure to Non-Lethal Levels of Metallic Nanoparticles
In order to investigate the effects of metallic nanoparticles (NPs) on the performance of in vitro bioassay, zinc oxide NP (ZnO NP), aluminum oxide NP (Al<sub>2</sub>O<sub>3</sub> NP), bare silver NP (Ag NP), and Ag NP capped with citrate (Ag<sup>cit</sup> NP) wer...
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MDPI AG
2020-05-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/11/3796 |
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| author | Byoung-cheun Lee Cuong N. Duong Jungkon Kim Suejin Kim Ig-chun Eom Pilje Kim |
| author_facet | Byoung-cheun Lee Cuong N. Duong Jungkon Kim Suejin Kim Ig-chun Eom Pilje Kim |
| author_sort | Byoung-cheun Lee |
| collection | DOAJ |
| description | In order to investigate the effects of metallic nanoparticles (NPs) on the performance of in vitro bioassay, zinc oxide NP (ZnO NP), aluminum oxide NP (Al<sub>2</sub>O<sub>3</sub> NP), bare silver NP (Ag NP), and Ag NP capped with citrate (Ag<sup>cit</sup> NP) were evaluated with yeast (<i>Saccharomyces cerevisiae</i> Y190) two-hybrid system (YES assay), carrying Japanese medaka estrogen receptors (mERs) in the presence of 17β-estradiol (E2, 10<sup>−6</sup> M), a reference chemical for estrogenic activity. The distribution of NPs in the yeast was also examined by field-emission transmission electron microscopy (FE-TEM). The results show that TEM analysis revealed that NPs were present inside the yeast and accumulated deep inside the cell organelles, suggesting that cell death was caused by NPs. However, despite no significant change of mortality, the E2 estrogenic activities in yeast exposed to ZnO NP and Al<sub>2</sub>O<sub>3</sub> NP were dose-dependently reduced. For Ag NP and Ag<sup>cit</sup> NP, such phenomenon observed in the exposure of ZnO NP and Al<sub>2</sub>O<sub>3</sub> NP did not occur. From the observations, we found that ZnO NP and Al<sub>2</sub>O<sub>3</sub> NP in the environmental media could result in underestimated estrogenicity of endocrine-disrupting compounds when evaluated by YES assay. |
| first_indexed | 2024-03-10T19:29:33Z |
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| id | doaj.art-500c91f3993d4d3d871841a3b8b10027 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T19:29:33Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-500c91f3993d4d3d871841a3b8b100272023-11-20T02:13:09ZengMDPI AGApplied Sciences2076-34172020-05-011011379610.3390/app10113796Inhibition of Estrogenic Response of Yeast Screen Assay by Exposure to Non-Lethal Levels of Metallic NanoparticlesByoung-cheun Lee0Cuong N. Duong1Jungkon Kim2Suejin Kim3Ig-chun Eom4Pilje Kim5Risk Assessment Division, National Institute of Environmental Research, Incheon 22689, KoreaRisk Assessment Division, National Institute of Environmental Research, Incheon 22689, KoreaRisk Assessment Division, National Institute of Environmental Research, Incheon 22689, KoreaRisk Assessment Division, National Institute of Environmental Research, Incheon 22689, KoreaRisk Assessment Division, National Institute of Environmental Research, Incheon 22689, KoreaRisk Assessment Division, National Institute of Environmental Research, Incheon 22689, KoreaIn order to investigate the effects of metallic nanoparticles (NPs) on the performance of in vitro bioassay, zinc oxide NP (ZnO NP), aluminum oxide NP (Al<sub>2</sub>O<sub>3</sub> NP), bare silver NP (Ag NP), and Ag NP capped with citrate (Ag<sup>cit</sup> NP) were evaluated with yeast (<i>Saccharomyces cerevisiae</i> Y190) two-hybrid system (YES assay), carrying Japanese medaka estrogen receptors (mERs) in the presence of 17β-estradiol (E2, 10<sup>−6</sup> M), a reference chemical for estrogenic activity. The distribution of NPs in the yeast was also examined by field-emission transmission electron microscopy (FE-TEM). The results show that TEM analysis revealed that NPs were present inside the yeast and accumulated deep inside the cell organelles, suggesting that cell death was caused by NPs. However, despite no significant change of mortality, the E2 estrogenic activities in yeast exposed to ZnO NP and Al<sub>2</sub>O<sub>3</sub> NP were dose-dependently reduced. For Ag NP and Ag<sup>cit</sup> NP, such phenomenon observed in the exposure of ZnO NP and Al<sub>2</sub>O<sub>3</sub> NP did not occur. From the observations, we found that ZnO NP and Al<sub>2</sub>O<sub>3</sub> NP in the environmental media could result in underestimated estrogenicity of endocrine-disrupting compounds when evaluated by YES assay.https://www.mdpi.com/2076-3417/10/11/3796nanoparticlesestrogen receptorJapanese medakareceptor binding assayyeast |
| spellingShingle | Byoung-cheun Lee Cuong N. Duong Jungkon Kim Suejin Kim Ig-chun Eom Pilje Kim Inhibition of Estrogenic Response of Yeast Screen Assay by Exposure to Non-Lethal Levels of Metallic Nanoparticles Applied Sciences nanoparticles estrogen receptor Japanese medaka receptor binding assay yeast |
| title | Inhibition of Estrogenic Response of Yeast Screen Assay by Exposure to Non-Lethal Levels of Metallic Nanoparticles |
| title_full | Inhibition of Estrogenic Response of Yeast Screen Assay by Exposure to Non-Lethal Levels of Metallic Nanoparticles |
| title_fullStr | Inhibition of Estrogenic Response of Yeast Screen Assay by Exposure to Non-Lethal Levels of Metallic Nanoparticles |
| title_full_unstemmed | Inhibition of Estrogenic Response of Yeast Screen Assay by Exposure to Non-Lethal Levels of Metallic Nanoparticles |
| title_short | Inhibition of Estrogenic Response of Yeast Screen Assay by Exposure to Non-Lethal Levels of Metallic Nanoparticles |
| title_sort | inhibition of estrogenic response of yeast screen assay by exposure to non lethal levels of metallic nanoparticles |
| topic | nanoparticles estrogen receptor Japanese medaka receptor binding assay yeast |
| url | https://www.mdpi.com/2076-3417/10/11/3796 |
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