Osteoconductivity and neurotoxicity of silver‐containing hydroxyapatite coating cage for spinal interbody fusion in rats
Abstract Background The use of spinal instrumentation is an established risk factor for postoperative infection. To address this problem, we prepared silver‐containing hydroxyapatite coating, consisting of highly osteoconductive hydroxyapatite interfused with silver. The technology has been adopted...
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| Format: | Article |
| Language: | English |
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Wiley
2023-03-01
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| Series: | JOR Spine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/jsp2.1236 |
| _version_ | 1827979066151534592 |
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| author | Takema Nakashima Tadatsugu Morimoto Akira Hashimoto Sakumo Kii Masatsugu Tsukamoto Hiroshi Miyamoto Mitsugu Todo Motoki Sonohata Masaaki Mawatari |
| author_facet | Takema Nakashima Tadatsugu Morimoto Akira Hashimoto Sakumo Kii Masatsugu Tsukamoto Hiroshi Miyamoto Mitsugu Todo Motoki Sonohata Masaaki Mawatari |
| author_sort | Takema Nakashima |
| collection | DOAJ |
| description | Abstract Background The use of spinal instrumentation is an established risk factor for postoperative infection. To address this problem, we prepared silver‐containing hydroxyapatite coating, consisting of highly osteoconductive hydroxyapatite interfused with silver. The technology has been adopted for total hip arthroplasty. Silver‐containing hydroxyapatite coating has been reported to have good biocompatibility and low toxicity. However, no studies about applying this coating in spinal surgery have addressed the osteoconductivity and direct neurotoxicity to the spinal cord of silver‐containing hydroxyapatite cages in spinal interbody fusion. Aim In this study, we evaluated the osteoconductivity and neurotoxicity of silver‐containing hydroxyapatite‐coated implants in rats. Materials & Methods Titanium (non‐coated, hydroxyapatite‐coated, and silver‐containing hydroxyapatite‐coated) interbody cages were inserted into the spine for anterior lumbar fusion. At 8 weeks postoperatively, micro‐computed tomography and histology were performed to evaluate the osteoconductivity of the cage. Inclined plane test and toe pinch test were performed postoperatively to assess neurotoxicity. Results Micro‐computed tomography data indicated no significant difference in bone volume/total volume among the three groups. Histologically, the hydroxyapatite‐coated and silver‐containing hydroxyapatite‐coated groups showed significantly higher bone contact rate than that of the titanium group. In contrast, there was no significant difference in bone formation rate among the three groups. Data of inclined plane and toe pinch test showed no significant loss of motor and sensory function in the three groups. Furthermore, there was no degeneration, necrosis, or accumulation of silver in the spinal cord on histology. Conclusions This study suggests that silver‐hydroxyapatite‐coated interbody cages produce good osteoconductivity and are not associated with direct neurotoxicity. |
| first_indexed | 2024-04-09T21:31:21Z |
| format | Article |
| id | doaj.art-500eea27ca264479b2ee0d54fceb866a |
| institution | Directory Open Access Journal |
| issn | 2572-1143 |
| language | English |
| last_indexed | 2024-04-09T21:31:21Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | JOR Spine |
| spelling | doaj.art-500eea27ca264479b2ee0d54fceb866a2023-03-27T08:33:01ZengWileyJOR Spine2572-11432023-03-0161n/an/a10.1002/jsp2.1236Osteoconductivity and neurotoxicity of silver‐containing hydroxyapatite coating cage for spinal interbody fusion in ratsTakema Nakashima0Tadatsugu Morimoto1Akira Hashimoto2Sakumo Kii3Masatsugu Tsukamoto4Hiroshi Miyamoto5Mitsugu Todo6Motoki Sonohata7Masaaki Mawatari8Department of Orthopaedic Surgery, Faculty of Medicine Saga University Saga JapanDepartment of Orthopaedic Surgery, Faculty of Medicine Saga University Saga JapanDepartment of Orthopaedic Surgery, Faculty of Medicine Saga University Saga JapanDepartment of Orthopaedic Surgery, Faculty of Medicine Saga University Saga JapanDepartment of Orthopaedic Surgery, Faculty of Medicine Saga University Saga JapanDepartment of Pathology and Microbiology, Faculty of Medicine Saga University Saga JapanDivision of Renewable Energy Dynamics, Research Institute for Applied Mechanics Kyushu University Fukuoka JapanDepartment of Orthopaedic Surgery, Faculty of Medicine Saga University Saga JapanDepartment of Orthopaedic Surgery, Faculty of Medicine Saga University Saga JapanAbstract Background The use of spinal instrumentation is an established risk factor for postoperative infection. To address this problem, we prepared silver‐containing hydroxyapatite coating, consisting of highly osteoconductive hydroxyapatite interfused with silver. The technology has been adopted for total hip arthroplasty. Silver‐containing hydroxyapatite coating has been reported to have good biocompatibility and low toxicity. However, no studies about applying this coating in spinal surgery have addressed the osteoconductivity and direct neurotoxicity to the spinal cord of silver‐containing hydroxyapatite cages in spinal interbody fusion. Aim In this study, we evaluated the osteoconductivity and neurotoxicity of silver‐containing hydroxyapatite‐coated implants in rats. Materials & Methods Titanium (non‐coated, hydroxyapatite‐coated, and silver‐containing hydroxyapatite‐coated) interbody cages were inserted into the spine for anterior lumbar fusion. At 8 weeks postoperatively, micro‐computed tomography and histology were performed to evaluate the osteoconductivity of the cage. Inclined plane test and toe pinch test were performed postoperatively to assess neurotoxicity. Results Micro‐computed tomography data indicated no significant difference in bone volume/total volume among the three groups. Histologically, the hydroxyapatite‐coated and silver‐containing hydroxyapatite‐coated groups showed significantly higher bone contact rate than that of the titanium group. In contrast, there was no significant difference in bone formation rate among the three groups. Data of inclined plane and toe pinch test showed no significant loss of motor and sensory function in the three groups. Furthermore, there was no degeneration, necrosis, or accumulation of silver in the spinal cord on histology. Conclusions This study suggests that silver‐hydroxyapatite‐coated interbody cages produce good osteoconductivity and are not associated with direct neurotoxicity.https://doi.org/10.1002/jsp2.1236hydroxyapatitelumbar spinal interbody fusionneurotoxicityosteoconductivitysilver |
| spellingShingle | Takema Nakashima Tadatsugu Morimoto Akira Hashimoto Sakumo Kii Masatsugu Tsukamoto Hiroshi Miyamoto Mitsugu Todo Motoki Sonohata Masaaki Mawatari Osteoconductivity and neurotoxicity of silver‐containing hydroxyapatite coating cage for spinal interbody fusion in rats JOR Spine hydroxyapatite lumbar spinal interbody fusion neurotoxicity osteoconductivity silver |
| title | Osteoconductivity and neurotoxicity of silver‐containing hydroxyapatite coating cage for spinal interbody fusion in rats |
| title_full | Osteoconductivity and neurotoxicity of silver‐containing hydroxyapatite coating cage for spinal interbody fusion in rats |
| title_fullStr | Osteoconductivity and neurotoxicity of silver‐containing hydroxyapatite coating cage for spinal interbody fusion in rats |
| title_full_unstemmed | Osteoconductivity and neurotoxicity of silver‐containing hydroxyapatite coating cage for spinal interbody fusion in rats |
| title_short | Osteoconductivity and neurotoxicity of silver‐containing hydroxyapatite coating cage for spinal interbody fusion in rats |
| title_sort | osteoconductivity and neurotoxicity of silver containing hydroxyapatite coating cage for spinal interbody fusion in rats |
| topic | hydroxyapatite lumbar spinal interbody fusion neurotoxicity osteoconductivity silver |
| url | https://doi.org/10.1002/jsp2.1236 |
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