Evaluation of Bio-Rad D-10® and arkray adams HA-8160® HPLC analyzers in HbA1c measurement
Glycosylated hemoglobin A1c (HbA1c) is an important parameter used for the assessment of time-dependent glycemic status and the diagnosis and follow-up of diabetes. This study aimed to evaluate the analytical performance of two HPLC analyzers, the Bio-Rad D-10®, and the Arkray Adams HA-8160®. Accura...
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| Format: | Article |
| Language: | English |
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Society of Turaz Bilim
2019-12-01
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| Series: | Medicine Science |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=41188 |
| _version_ | 1797328448934379520 |
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| author | Ozgur Mehmet Yis Mine Busra Pehlivan Guler Bugdayci Neslihan Yuce |
| author_facet | Ozgur Mehmet Yis Mine Busra Pehlivan Guler Bugdayci Neslihan Yuce |
| author_sort | Ozgur Mehmet Yis |
| collection | DOAJ |
| description | Glycosylated hemoglobin A1c (HbA1c) is an important parameter used for the assessment of time-dependent glycemic status and the diagnosis and follow-up of diabetes. This study aimed to evaluate the analytical performance of two HPLC analyzers, the Bio-Rad D-10®, and the Arkray Adams HA-8160®. Accuracy and imprecision studies were conducted, and a method comparison study was performed with 105 samples. Samples were collected on five consecutive days and measured on both analyzers within two hours. Bland-Altman and regression analyses were used for statistical evaluation of the data. Low and high-level within-day CV values were calculated as 1.22% and 0.60% for the Arkray analyzer and 1.2% and 0.30% for the Bio-Rad analyzer, respectively. They were calculated as 1.27% and 2.52% for the Arkray analyzer and 2.32% and 3.44% for the Bio-Rad analyzer, respectively, between days. The within-day CV values for both analyzers were below the limit of 2.5% specified by the International Federation of Clinical Chemistry (IFCC). The bias average for three months was 3.2% for the Arkray analyzer and 1.2% for the Bio-Rad analyzer. The Spearman correlation coefficient (r) was 0.973 (p [Med-Science 2019; 8(4.000): 953-6] |
| first_indexed | 2024-03-08T06:51:57Z |
| format | Article |
| id | doaj.art-50139f5c462646ecaee889d821fe15ad |
| institution | Directory Open Access Journal |
| issn | 2147-0634 |
| language | English |
| last_indexed | 2024-03-08T06:51:57Z |
| publishDate | 2019-12-01 |
| publisher | Society of Turaz Bilim |
| record_format | Article |
| series | Medicine Science |
| spelling | doaj.art-50139f5c462646ecaee889d821fe15ad2024-02-03T06:40:33ZengSociety of Turaz BilimMedicine Science2147-06342019-12-0184953610.5455/medscience.2019.08.908441188Evaluation of Bio-Rad D-10® and arkray adams HA-8160® HPLC analyzers in HbA1c measurementOzgur Mehmet Yis0Mine Busra PehlivanGuler BugdayciNeslihan YuceBolu Abant Izzet Baysal University Medical Faculty, Department of Biochemistry, 14280 Golkoy, Bolu, Turkey Ataturk University, Medical Faculty, Department of Medical Biochemistry, Erzurum, TurkeyGlycosylated hemoglobin A1c (HbA1c) is an important parameter used for the assessment of time-dependent glycemic status and the diagnosis and follow-up of diabetes. This study aimed to evaluate the analytical performance of two HPLC analyzers, the Bio-Rad D-10®, and the Arkray Adams HA-8160®. Accuracy and imprecision studies were conducted, and a method comparison study was performed with 105 samples. Samples were collected on five consecutive days and measured on both analyzers within two hours. Bland-Altman and regression analyses were used for statistical evaluation of the data. Low and high-level within-day CV values were calculated as 1.22% and 0.60% for the Arkray analyzer and 1.2% and 0.30% for the Bio-Rad analyzer, respectively. They were calculated as 1.27% and 2.52% for the Arkray analyzer and 2.32% and 3.44% for the Bio-Rad analyzer, respectively, between days. The within-day CV values for both analyzers were below the limit of 2.5% specified by the International Federation of Clinical Chemistry (IFCC). The bias average for three months was 3.2% for the Arkray analyzer and 1.2% for the Bio-Rad analyzer. The Spearman correlation coefficient (r) was 0.973 (p [Med-Science 2019; 8(4.000): 953-6]http://www.ejmanager.com/fulltextpdf.php?mno=41188diabetes mellitushba1chplcanalytical performance |
| spellingShingle | Ozgur Mehmet Yis Mine Busra Pehlivan Guler Bugdayci Neslihan Yuce Evaluation of Bio-Rad D-10® and arkray adams HA-8160® HPLC analyzers in HbA1c measurement Medicine Science diabetes mellitus hba1c hplc analytical performance |
| title | Evaluation of Bio-Rad D-10® and arkray adams HA-8160® HPLC analyzers in HbA1c measurement |
| title_full | Evaluation of Bio-Rad D-10® and arkray adams HA-8160® HPLC analyzers in HbA1c measurement |
| title_fullStr | Evaluation of Bio-Rad D-10® and arkray adams HA-8160® HPLC analyzers in HbA1c measurement |
| title_full_unstemmed | Evaluation of Bio-Rad D-10® and arkray adams HA-8160® HPLC analyzers in HbA1c measurement |
| title_short | Evaluation of Bio-Rad D-10® and arkray adams HA-8160® HPLC analyzers in HbA1c measurement |
| title_sort | evaluation of bio rad d 10 r and arkray adams ha 8160 r hplc analyzers in hba1c measurement |
| topic | diabetes mellitus hba1c hplc analytical performance |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=41188 |
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