Detection of <i>Brucella abortus</i> in Chiredzi district in Zimbabwe

Brucellosis is an endemic disease in Zimbabwe caused by the genus <em>Brucella</em>. <em>Brucella</em> seroprevalence was recently reported to be high in the wildlife-livestock interface in the Chiredzi district and the neighbouring Gonarezhou National Park (GNP) in Zimba...

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Main Authors: Calvin Gomo, Shuvai Musari, Michel de Garine-Wichatitsky, Alexandre Caron, Davies M. Pfukenyi, Henriette van Heerden
Format: Article
Language:English
Published: AOSIS 2012-02-01
Series:Onderstepoort Journal of Veterinary Research
Subjects:
Online Access:http://www.ojvr.org/index.php/ojvr/article/view/417
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author Calvin Gomo
Shuvai Musari
Michel de Garine-Wichatitsky
Alexandre Caron
Davies M. Pfukenyi
Henriette van Heerden
author_facet Calvin Gomo
Shuvai Musari
Michel de Garine-Wichatitsky
Alexandre Caron
Davies M. Pfukenyi
Henriette van Heerden
author_sort Calvin Gomo
collection DOAJ
description Brucellosis is an endemic disease in Zimbabwe caused by the genus <em>Brucella</em>. <em>Brucella</em> seroprevalence was recently reported to be high in the wildlife-livestock interface in the Chiredzi district and the neighbouring Gonarezhou National Park (GNP) in Zimbabwe, and higher amongst communal cattle with an abortion history and access to grazing in GNP than amongst communal cattle with no abortion history or access to grazing in GNP. The aim of this study was to investigate <em>Brucella</em> species in brucellosis seropositive cattle in the Chiredzi district with access to GNP using isolation and identification. Isolation of<em> Brucella </em>species from whole blood (<em>n</em> = 18) and milk samples (<em>n</em> = 10) from seropositive animals with an abortion history was based on the rose Bengal test (RBT) and enzyme-linked immunoassays (enzyme- linked immunosorbent assay [ELISA]; indirect ELISA and complement ELISA), using microbiology and polymerase chain reaction (PCR) methods. <em>Brucella abortus</em> was cultured and identified from blood and milk collected from seropositive cows in both communal areas. The<em> Brucella</em>-specific 16-23S intergenic spacer (ITS) PCR and multiplex AMOS-PCR assays verified the identification of the cultures. Our results confirmed that <em>B. abortus</em> is present in cattle on communal farms in the Chiredzi district in Zimbabwe and might cause cattle abortions. The need for implementing control measures and raising public awareness on zoonotic transmission of brucellosis are recommended.
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spelling doaj.art-50150ab3df494ce8838a23a552d400482022-12-21T17:57:51ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352012-02-01791e1e5389Detection of <i>Brucella abortus</i> in Chiredzi district in ZimbabweCalvin Gomo0Shuvai Musari1Michel de Garine-Wichatitsky2Alexandre Caron3Davies M. Pfukenyi4Henriette van Heerden5Department of Veterinary Tropical Diseases, University of Pretoria, South Africa; Central Veterinary Laboratory, Harare, ZimbabweCentral Veterinary Laboratory, HarareCIRAD, UPR AGIR, Department Environment and Societies, Harare, Zimbabwe; CIRAD, UPR AGIR, Department Environment and Societies, Montpellier, FranceCIRAD, UPR AGIR, Department Environment and Societies, Harare, Zimbabwe; CIRAD, UPR AGIR, Department Environment and Societies, Montpellier, FranceFaculty of Veterinary Science, University of ZimbabweDepartment of Veterinary Tropical Diseases, University of PretoriaBrucellosis is an endemic disease in Zimbabwe caused by the genus <em>Brucella</em>. <em>Brucella</em> seroprevalence was recently reported to be high in the wildlife-livestock interface in the Chiredzi district and the neighbouring Gonarezhou National Park (GNP) in Zimbabwe, and higher amongst communal cattle with an abortion history and access to grazing in GNP than amongst communal cattle with no abortion history or access to grazing in GNP. The aim of this study was to investigate <em>Brucella</em> species in brucellosis seropositive cattle in the Chiredzi district with access to GNP using isolation and identification. Isolation of<em> Brucella </em>species from whole blood (<em>n</em> = 18) and milk samples (<em>n</em> = 10) from seropositive animals with an abortion history was based on the rose Bengal test (RBT) and enzyme-linked immunoassays (enzyme- linked immunosorbent assay [ELISA]; indirect ELISA and complement ELISA), using microbiology and polymerase chain reaction (PCR) methods. <em>Brucella abortus</em> was cultured and identified from blood and milk collected from seropositive cows in both communal areas. The<em> Brucella</em>-specific 16-23S intergenic spacer (ITS) PCR and multiplex AMOS-PCR assays verified the identification of the cultures. Our results confirmed that <em>B. abortus</em> is present in cattle on communal farms in the Chiredzi district in Zimbabwe and might cause cattle abortions. The need for implementing control measures and raising public awareness on zoonotic transmission of brucellosis are recommended.http://www.ojvr.org/index.php/ojvr/article/view/417BrucellabrucellosisserologyPCRcultureblood
spellingShingle Calvin Gomo
Shuvai Musari
Michel de Garine-Wichatitsky
Alexandre Caron
Davies M. Pfukenyi
Henriette van Heerden
Detection of <i>Brucella abortus</i> in Chiredzi district in Zimbabwe
Onderstepoort Journal of Veterinary Research
Brucella
brucellosis
serology
PCR
culture
blood
title Detection of <i>Brucella abortus</i> in Chiredzi district in Zimbabwe
title_full Detection of <i>Brucella abortus</i> in Chiredzi district in Zimbabwe
title_fullStr Detection of <i>Brucella abortus</i> in Chiredzi district in Zimbabwe
title_full_unstemmed Detection of <i>Brucella abortus</i> in Chiredzi district in Zimbabwe
title_short Detection of <i>Brucella abortus</i> in Chiredzi district in Zimbabwe
title_sort detection of i brucella abortus i in chiredzi district in zimbabwe
topic Brucella
brucellosis
serology
PCR
culture
blood
url http://www.ojvr.org/index.php/ojvr/article/view/417
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