Effects of freezer storage time on levels of complement biomarkers
Abstract Background There is uncertainty regarding how stable complement analytes are during long-term storage at – 80 °C. As part of our work program we have measured 17 complement biomarkers (C1q, C1 inhibitor, C3, C3a, iC3b, C4, C5, C9, FB, FD, FH, FI, TCC, Bb, sCR1, sCR2, Clusterin) and the benc...
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| Format: | Article |
| Language: | English |
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BMC
2017-11-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-017-2885-1 |
| _version_ | 1818521327201419264 |
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| author | Angharad R. Morgan Caroline O’Hagan Samuel Touchard Simon Lovestone B. Paul Morgan |
| author_facet | Angharad R. Morgan Caroline O’Hagan Samuel Touchard Simon Lovestone B. Paul Morgan |
| author_sort | Angharad R. Morgan |
| collection | DOAJ |
| description | Abstract Background There is uncertainty regarding how stable complement analytes are during long-term storage at – 80 °C. As part of our work program we have measured 17 complement biomarkers (C1q, C1 inhibitor, C3, C3a, iC3b, C4, C5, C9, FB, FD, FH, FI, TCC, Bb, sCR1, sCR2, Clusterin) and the benchmark inflammatory marker C-reactive protein (CRP) in a large set of plasma samples (n = 720) that had been collected, processed and subsequently stored at – 80 °C over a period of 6.6–10.6 years, prior to laboratory analysis. The biomarkers were measured using solid-phase enzyme immunoassays with a combination of multiplex assays using the MesoScale Discovery Platform and single-plex enzyme-linked immunosorbent assays (ELISAs). As part of a post hoc analysis of extrinsic factors (co-variables) affecting the analyses we investigated the impact of freezer storage time on the values obtained for each complement analyte. Results With the exception of five analytes (C4, C9, sCR2, clusterin and CRP), storage time was significantly correlated with measured plasma concentrations. For ten analytes: C3, FI, FB, FD, C5, sCR1, C3a, iC3b, Bb and TCC, storage time was positively correlated with concentration and for three analytes: FH, C1q, and C1 inhibitor, storage time was negatively correlated with concentration. Conclusions The results suggest that information on storage time should be regarded as an important co-variable and taken into consideration when analysing data to look for associations of complement biomarker levels and disease or other outcomes. |
| first_indexed | 2024-12-11T01:49:32Z |
| format | Article |
| id | doaj.art-501574c0d15e46c4ad3592faa3d140d8 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-11T01:49:32Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-501574c0d15e46c4ad3592faa3d140d82022-12-22T01:24:47ZengBMCBMC Research Notes1756-05002017-11-011011510.1186/s13104-017-2885-1Effects of freezer storage time on levels of complement biomarkersAngharad R. Morgan0Caroline O’Hagan1Samuel Touchard2Simon Lovestone3B. Paul Morgan4Division of Infection and Immunity, School of Medicine, Cardiff University, Heath ParkDivision of Infection and Immunity, School of Medicine, Cardiff University, Heath ParkDivision of Infection and Immunity, School of Medicine, Cardiff University, Heath ParkDepartment of Psychiatry, University of OxfordDivision of Infection and Immunity, School of Medicine, Cardiff University, Heath ParkAbstract Background There is uncertainty regarding how stable complement analytes are during long-term storage at – 80 °C. As part of our work program we have measured 17 complement biomarkers (C1q, C1 inhibitor, C3, C3a, iC3b, C4, C5, C9, FB, FD, FH, FI, TCC, Bb, sCR1, sCR2, Clusterin) and the benchmark inflammatory marker C-reactive protein (CRP) in a large set of plasma samples (n = 720) that had been collected, processed and subsequently stored at – 80 °C over a period of 6.6–10.6 years, prior to laboratory analysis. The biomarkers were measured using solid-phase enzyme immunoassays with a combination of multiplex assays using the MesoScale Discovery Platform and single-plex enzyme-linked immunosorbent assays (ELISAs). As part of a post hoc analysis of extrinsic factors (co-variables) affecting the analyses we investigated the impact of freezer storage time on the values obtained for each complement analyte. Results With the exception of five analytes (C4, C9, sCR2, clusterin and CRP), storage time was significantly correlated with measured plasma concentrations. For ten analytes: C3, FI, FB, FD, C5, sCR1, C3a, iC3b, Bb and TCC, storage time was positively correlated with concentration and for three analytes: FH, C1q, and C1 inhibitor, storage time was negatively correlated with concentration. Conclusions The results suggest that information on storage time should be regarded as an important co-variable and taken into consideration when analysing data to look for associations of complement biomarker levels and disease or other outcomes.http://link.springer.com/article/10.1186/s13104-017-2885-1ComplementBiomarkerPlasma− 80 °C storage |
| spellingShingle | Angharad R. Morgan Caroline O’Hagan Samuel Touchard Simon Lovestone B. Paul Morgan Effects of freezer storage time on levels of complement biomarkers BMC Research Notes Complement Biomarker Plasma − 80 °C storage |
| title | Effects of freezer storage time on levels of complement biomarkers |
| title_full | Effects of freezer storage time on levels of complement biomarkers |
| title_fullStr | Effects of freezer storage time on levels of complement biomarkers |
| title_full_unstemmed | Effects of freezer storage time on levels of complement biomarkers |
| title_short | Effects of freezer storage time on levels of complement biomarkers |
| title_sort | effects of freezer storage time on levels of complement biomarkers |
| topic | Complement Biomarker Plasma − 80 °C storage |
| url | http://link.springer.com/article/10.1186/s13104-017-2885-1 |
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