A Glutathione Peroxidase Gene from <i>Litopenaeus vannamei</i> Is Involved in Oxidative Stress Responses and Pathogen Infection Resistance
In shrimp, several glutathione peroxidase (GPX) genes have been cloned and functionally studied. Increasing evidence suggests the genes’ involvement in white spot syndrome virus (WSSV)- or <i>Vibrio alginolyticus</i>-infection resistance. In the present study, a novel GXP gene (<i>...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/1/567 |
| Summary: | In shrimp, several glutathione peroxidase (GPX) genes have been cloned and functionally studied. Increasing evidence suggests the genes’ involvement in white spot syndrome virus (WSSV)- or <i>Vibrio alginolyticus</i>-infection resistance. In the present study, a novel GXP gene (<i>LvGPX3</i>) was cloned in <i>Litopenaeus vannamei</i>. Promoter of <i>LvGPX3</i> was activated by NF-E2-related factor 2. Further study showed that <i>LvGPX3</i> expression was evidently accelerated by oxidative stress or WSSV or <i>V. alginolyticus</i> infection. Consistently, downregulated expression of <i>LvGPX3</i> increased the cumulative mortality of WSSV- or <i>V. alginolyticus</i>-infected shrimp. Similar results occurred in shrimp suffering from oxidative stress. Moreover, LvGPX3 was important for enhancing <i>Antimicrobial peptide</i> (<i>AMP</i>) gene expression in S2 cells with lipopolysaccharide treatment. Further, knockdown of <i>LvGPX3</i> expression significantly suppressed expression of AMPs, such as <i>Penaeidins 2a</i>, <i>Penaeidins 3a</i> and <i>anti-lipopolysaccharide factor 1</i> in shrimp. AMPs have been proven to be engaged in shrimp WSSV- or <i>V. alginolyticus</i>-infection resistance; it was inferred that LvGPX3 might enhance shrimp immune response under immune challenges, such as increasing expression of AMPs. The regulation mechanism remains to be further studied. |
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| ISSN: | 1661-6596 1422-0067 |