Genetic polymorphism of vitamin D receptors and plasminogen activator inhibitor‐1 and osteonecrosis risk in childhood acute lymphoblastic leukemia
Abstract Background Osteonecrosis (ON) is one of the major therapy‐related complications in childhood acute lymphoblastic leukemia (ALL). The purpose of the current study is to assess the frequency of ON in children with ALL and to detect whether polymorphisms in vitamin D receptor gene (VDR) and pl...
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| Format: | Article |
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Wiley
2021-07-01
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| Series: | Molecular Genetics & Genomic Medicine |
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| Online Access: | https://doi.org/10.1002/mgg3.1700 |
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| author | Laila M. Sherief Mohamed Beshir Nermin Raafat Elhamy R. Abdelkhalek Wesam A. Mokhtar Khaled M. Elgerby Basma K. Soliman Hosam E. Salah Ghada A. Mokhtar Naglaa M. Kamal Heba ELsayed Marwa Zakaria |
| author_facet | Laila M. Sherief Mohamed Beshir Nermin Raafat Elhamy R. Abdelkhalek Wesam A. Mokhtar Khaled M. Elgerby Basma K. Soliman Hosam E. Salah Ghada A. Mokhtar Naglaa M. Kamal Heba ELsayed Marwa Zakaria |
| author_sort | Laila M. Sherief |
| collection | DOAJ |
| description | Abstract Background Osteonecrosis (ON) is one of the major therapy‐related complications in childhood acute lymphoblastic leukemia (ALL). The purpose of the current study is to assess the frequency of ON in children with ALL and to detect whether polymorphisms in vitamin D receptor gene (VDR) and plasminogen activator inhibitor‐1 (PAI‐1) gene can affect the risk of ON. Patients and Methods Nighty‐six ALL children were enrolled. Serum 25‐hydroxyvitamin D 25(OH)D levels were performed in addition to the detection of polymorphisms in PAI‐1and VDR genes by polymerase chain reaction. Results Ten out of 96 patients had ON (four males and six females aged above 10 years) and had an insufficient level of 25(OH)D. Fifty‐two percent of patients had PAI‐1 GG genotype while 48% had PAI‐1 GA genotype. PAI‐1 polymorphism was detected in 60% of all ON cases. The frequencies of VDR genotypes were CT (56.3%), CC (39.6%), and TT (4.2%). Osteonecrosis was found in eight patients with CC genotype and in two patients with CT genotype. Conclusion Osteonecrosis can develop early during the therapy of ALL. Older age and insufficient level of 25(OH)D were considered important risk factor for the development of osteonecrosis. PAT‐1 and VDR gene polymorphism may be a genetic risk factor in its pathogenesis. |
| first_indexed | 2024-12-16T11:22:32Z |
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| institution | Directory Open Access Journal |
| issn | 2324-9269 |
| language | English |
| last_indexed | 2024-12-16T11:22:32Z |
| publishDate | 2021-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Molecular Genetics & Genomic Medicine |
| spelling | doaj.art-50185a15b44e4cadad9f68749736c8e12022-12-21T22:33:26ZengWileyMolecular Genetics & Genomic Medicine2324-92692021-07-0197n/an/a10.1002/mgg3.1700Genetic polymorphism of vitamin D receptors and plasminogen activator inhibitor‐1 and osteonecrosis risk in childhood acute lymphoblastic leukemiaLaila M. Sherief0Mohamed Beshir1Nermin Raafat2Elhamy R. Abdelkhalek3Wesam A. Mokhtar4Khaled M. Elgerby5Basma K. Soliman6Hosam E. Salah7Ghada A. Mokhtar8Naglaa M. Kamal9Heba ELsayed10Marwa Zakaria11Department of Pediatrics Faculty of Medicine Zagazig University Zagazig EgyptDepartment of Pediatrics Faculty of Medicine Zagazig University Zagazig EgyptDepartment of Medical Biochemistry Faculty of Medicine Zagazig University Zagazig EgyptDepartment of Pediatrics Faculty of Medicine Zagazig University Zagazig EgyptDepartment of Pediatrics Faculty of Medicine Zagazig University Zagazig EgyptDepartment of Radiodiagnosis Faculty of Medicine Zagazig University Zagazig EgyptDepartment of Radiodiagnosis Faculty of Medicine Zagazig University Zagazig EgyptDepartment of Clinical Pathology Faculty of Medicine Zagazig University Zagazig EgyptDepartment of Medical Microbiology & Immunology Faculty of Medicine Zagazig University Zagazig EgyptPediatric Department Kasr Alainy Faculty of Medicine Cairo University Cairo EgyptDepartment of Pediatrics Faculty of Medicine Zagazig University Zagazig EgyptDepartment of Pediatrics Faculty of Medicine Zagazig University Zagazig EgyptAbstract Background Osteonecrosis (ON) is one of the major therapy‐related complications in childhood acute lymphoblastic leukemia (ALL). The purpose of the current study is to assess the frequency of ON in children with ALL and to detect whether polymorphisms in vitamin D receptor gene (VDR) and plasminogen activator inhibitor‐1 (PAI‐1) gene can affect the risk of ON. Patients and Methods Nighty‐six ALL children were enrolled. Serum 25‐hydroxyvitamin D 25(OH)D levels were performed in addition to the detection of polymorphisms in PAI‐1and VDR genes by polymerase chain reaction. Results Ten out of 96 patients had ON (four males and six females aged above 10 years) and had an insufficient level of 25(OH)D. Fifty‐two percent of patients had PAI‐1 GG genotype while 48% had PAI‐1 GA genotype. PAI‐1 polymorphism was detected in 60% of all ON cases. The frequencies of VDR genotypes were CT (56.3%), CC (39.6%), and TT (4.2%). Osteonecrosis was found in eight patients with CC genotype and in two patients with CT genotype. Conclusion Osteonecrosis can develop early during the therapy of ALL. Older age and insufficient level of 25(OH)D were considered important risk factor for the development of osteonecrosis. PAT‐1 and VDR gene polymorphism may be a genetic risk factor in its pathogenesis.https://doi.org/10.1002/mgg3.1700ALLosteonecrosisPAI‐1pediatricpolymorphismVDR |
| spellingShingle | Laila M. Sherief Mohamed Beshir Nermin Raafat Elhamy R. Abdelkhalek Wesam A. Mokhtar Khaled M. Elgerby Basma K. Soliman Hosam E. Salah Ghada A. Mokhtar Naglaa M. Kamal Heba ELsayed Marwa Zakaria Genetic polymorphism of vitamin D receptors and plasminogen activator inhibitor‐1 and osteonecrosis risk in childhood acute lymphoblastic leukemia Molecular Genetics & Genomic Medicine ALL osteonecrosis PAI‐1 pediatric polymorphism VDR |
| title | Genetic polymorphism of vitamin D receptors and plasminogen activator inhibitor‐1 and osteonecrosis risk in childhood acute lymphoblastic leukemia |
| title_full | Genetic polymorphism of vitamin D receptors and plasminogen activator inhibitor‐1 and osteonecrosis risk in childhood acute lymphoblastic leukemia |
| title_fullStr | Genetic polymorphism of vitamin D receptors and plasminogen activator inhibitor‐1 and osteonecrosis risk in childhood acute lymphoblastic leukemia |
| title_full_unstemmed | Genetic polymorphism of vitamin D receptors and plasminogen activator inhibitor‐1 and osteonecrosis risk in childhood acute lymphoblastic leukemia |
| title_short | Genetic polymorphism of vitamin D receptors and plasminogen activator inhibitor‐1 and osteonecrosis risk in childhood acute lymphoblastic leukemia |
| title_sort | genetic polymorphism of vitamin d receptors and plasminogen activator inhibitor 1 and osteonecrosis risk in childhood acute lymphoblastic leukemia |
| topic | ALL osteonecrosis PAI‐1 pediatric polymorphism VDR |
| url | https://doi.org/10.1002/mgg3.1700 |
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