PACT/PRKRA and p53 regulate transcriptional activity of DMRT1
Abstract The transcription factor DMRT1 (doublesex and mab-3 related transcription factor) has two distinct functions, somatic-cell masculinization and germ-cell development in some vertebrate species, including mouse and the African clawed frog Xenopus laevis. However, its transcriptional regulatio...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2020-03-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400702&tlng=en |
| _version_ | 1818988065533722624 |
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| author | Kazuko Fujitani Asako Otomo Yuto Nagayama Taro Tachibana Rika Kato Yusuke Kawashima Yoshio Kodera Tomoko Kato Shuji Takada Kei Tamura Nobuhiko Takamatsu Michihiko Ito |
| author_facet | Kazuko Fujitani Asako Otomo Yuto Nagayama Taro Tachibana Rika Kato Yusuke Kawashima Yoshio Kodera Tomoko Kato Shuji Takada Kei Tamura Nobuhiko Takamatsu Michihiko Ito |
| author_sort | Kazuko Fujitani |
| collection | DOAJ |
| description | Abstract The transcription factor DMRT1 (doublesex and mab-3 related transcription factor) has two distinct functions, somatic-cell masculinization and germ-cell development in some vertebrate species, including mouse and the African clawed frog Xenopus laevis. However, its transcriptional regulation remains unclear. We tried to identify DMRT1-interacting proteins from X. laevis testes by immunoprecipitation with an anti-DMRT1 antibody and MS/MS analysis, and selected three proteins, including PACT/PRKRA (Interferon-inducible double-stranded RNA dependent protein kinase activator A) derived from testes. Next, we examined the effects of PACT/PRKRA and/or p53 on the transcriptional activity of DMRT1. In transfected 293T cells, PACT/PRKRA and p53 significantly enhanced and repressed DMRT1-driven luciferase activity, respectively. We also observed that the enhanced activity by PACT/PRKRA was strongly attenuated by p53. Moreover, in situ hybridization analysis of Pact/Prkra mRNA in tadpole gonads indicated high expression in female and male germline stem cells. Taken together, these findings suggest that PACT/PRKRA and p53 might positively and negatively regulate the activity of DMRT1, respectively, for germline stem cell fate. |
| first_indexed | 2024-12-20T19:16:39Z |
| format | Article |
| id | doaj.art-501a9209faca4a77a18987add58d3303 |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-12-20T19:16:39Z |
| publishDate | 2020-03-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-501a9209faca4a77a18987add58d33032022-12-21T19:29:06ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852020-03-0143210.1590/1678-4685-gmb-2019-0017PACT/PRKRA and p53 regulate transcriptional activity of DMRT1Kazuko FujitaniAsako OtomoYuto NagayamaTaro TachibanaRika KatoYusuke KawashimaYoshio KoderaTomoko KatoShuji TakadaKei TamuraNobuhiko TakamatsuMichihiko Itohttps://orcid.org/0000-0003-1881-6916Abstract The transcription factor DMRT1 (doublesex and mab-3 related transcription factor) has two distinct functions, somatic-cell masculinization and germ-cell development in some vertebrate species, including mouse and the African clawed frog Xenopus laevis. However, its transcriptional regulation remains unclear. We tried to identify DMRT1-interacting proteins from X. laevis testes by immunoprecipitation with an anti-DMRT1 antibody and MS/MS analysis, and selected three proteins, including PACT/PRKRA (Interferon-inducible double-stranded RNA dependent protein kinase activator A) derived from testes. Next, we examined the effects of PACT/PRKRA and/or p53 on the transcriptional activity of DMRT1. In transfected 293T cells, PACT/PRKRA and p53 significantly enhanced and repressed DMRT1-driven luciferase activity, respectively. We also observed that the enhanced activity by PACT/PRKRA was strongly attenuated by p53. Moreover, in situ hybridization analysis of Pact/Prkra mRNA in tadpole gonads indicated high expression in female and male germline stem cells. Taken together, these findings suggest that PACT/PRKRA and p53 might positively and negatively regulate the activity of DMRT1, respectively, for germline stem cell fate.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400702&tlng=enp53DMRT1germline stem celltestisXenopus |
| spellingShingle | Kazuko Fujitani Asako Otomo Yuto Nagayama Taro Tachibana Rika Kato Yusuke Kawashima Yoshio Kodera Tomoko Kato Shuji Takada Kei Tamura Nobuhiko Takamatsu Michihiko Ito PACT/PRKRA and p53 regulate transcriptional activity of DMRT1 Genetics and Molecular Biology p53 DMRT1 germline stem cell testis Xenopus |
| title | PACT/PRKRA and p53 regulate transcriptional activity of DMRT1 |
| title_full | PACT/PRKRA and p53 regulate transcriptional activity of DMRT1 |
| title_fullStr | PACT/PRKRA and p53 regulate transcriptional activity of DMRT1 |
| title_full_unstemmed | PACT/PRKRA and p53 regulate transcriptional activity of DMRT1 |
| title_short | PACT/PRKRA and p53 regulate transcriptional activity of DMRT1 |
| title_sort | pact prkra and p53 regulate transcriptional activity of dmrt1 |
| topic | p53 DMRT1 germline stem cell testis Xenopus |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400702&tlng=en |
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