Burn-induced Oxidative Stress and Serum Glutathione Depletion; a Cross Sectional Study
Introduction: Several studies have shown the role of oxidative stress in pathophysiology of burn injuries. This study aimed to evaluate the changes of oxidant-antioxidant levels during the week following burn injuries and its correlation with grade of burn. Methods: In this prospective cross-secti...
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| Format: | Article |
| Language: | English |
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Shahid Beheshti University of Medical Sciences
2018-09-01
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| Series: | Emergency |
| Subjects: | |
| Online Access: | http://journals.sbmu.ac.ir/emergency/article/view/21125 |
| _version_ | 1818526378240245760 |
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| author | Arash Beiraghi-Toosi Roya Askarian Faezeh Sadrabadi Haghighi Mohammad Safarian Fereshteh Kalantari Seyed Isaac Hashemy |
| author_facet | Arash Beiraghi-Toosi Roya Askarian Faezeh Sadrabadi Haghighi Mohammad Safarian Fereshteh Kalantari Seyed Isaac Hashemy |
| author_sort | Arash Beiraghi-Toosi |
| collection | DOAJ |
| description | Introduction: Several studies have shown the role of oxidative stress in pathophysiology of burn injuries. This study aimed to evaluate the changes of oxidant-antioxidant levels during the week following burn injuries and its correlation with grade of burn.
Methods: In this prospective cross-sectional study, changes of total glutathione, reduced glutathione (GSH), oxidized GSH (GSSG), GSH/GSSG ratio, as well as Pro-oxidant-antioxidant balance (PAB) were investigated on the 1st, 2nd and 7th days of admission in patients with > 15 % burns.
Results: 40 patients with the mean age of 21.1 ± 14.5 were studied (47.5% male). More than 50% of patients were in the 18 – 55 years age range and over 70% had 20% – 60% grade of burn. Total serum glutathione level and GSH had significant decreasing trends (P < 0.001) and GSSG and GSH/GSSG ratio had increasing trends (p < 0.001). No significant correlation was observed between serum GSH level and the total body surface area (TBSA) of burn injury (r = 0.047; p = 0.779). The evaluation of PAB and its correlation with TBSA showed a significant and direct association between them on the 1st (coefficient = 0.516; p = 0.001), 2nd (coefficient = 0.62; p <0.001), and 3rd (coefficient = 0.471; p = 0.002) day of follow up.
Conclusion: According to this study, the redox perturbation occurred in burn injury which was measured and proved by decreased GSH/GSSG ratio as well as the shift of PAB in favour of oxidants. Besides, since PAB positively correlated with the severity of dermal damage, it might suggest the application of antioxidants as a part of therapeutic protocol for which the dosage should be proportionate to the surface area of the damaged skin. |
| first_indexed | 2024-12-11T06:22:09Z |
| format | Article |
| id | doaj.art-501ad23f7e2f4f49af88b30c5da5097b |
| institution | Directory Open Access Journal |
| issn | 2345-4563 2345-4571 |
| language | English |
| last_indexed | 2024-12-11T06:22:09Z |
| publishDate | 2018-09-01 |
| publisher | Shahid Beheshti University of Medical Sciences |
| record_format | Article |
| series | Emergency |
| spelling | doaj.art-501ad23f7e2f4f49af88b30c5da5097b2022-12-22T01:17:47ZengShahid Beheshti University of Medical SciencesEmergency2345-45632345-45712018-09-0161e54e5410.22037/emergency.v6i1.2112510795Burn-induced Oxidative Stress and Serum Glutathione Depletion; a Cross Sectional StudyArash Beiraghi-Toosi0Roya Askarian1Faezeh Sadrabadi Haghighi2Mohammad Safarian3Fereshteh Kalantari4Seyed Isaac Hashemy5Surgical Oncology Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran Department of Plastic Surgery, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranSurgical Oncology Research Centre, Mashhad University of Medical Sciences, Mashhad, IranSurgical Oncology Research Centre, Mashhad University of Medical Sciences, Mashhad, IranIntroduction: Several studies have shown the role of oxidative stress in pathophysiology of burn injuries. This study aimed to evaluate the changes of oxidant-antioxidant levels during the week following burn injuries and its correlation with grade of burn. Methods: In this prospective cross-sectional study, changes of total glutathione, reduced glutathione (GSH), oxidized GSH (GSSG), GSH/GSSG ratio, as well as Pro-oxidant-antioxidant balance (PAB) were investigated on the 1st, 2nd and 7th days of admission in patients with > 15 % burns. Results: 40 patients with the mean age of 21.1 ± 14.5 were studied (47.5% male). More than 50% of patients were in the 18 – 55 years age range and over 70% had 20% – 60% grade of burn. Total serum glutathione level and GSH had significant decreasing trends (P < 0.001) and GSSG and GSH/GSSG ratio had increasing trends (p < 0.001). No significant correlation was observed between serum GSH level and the total body surface area (TBSA) of burn injury (r = 0.047; p = 0.779). The evaluation of PAB and its correlation with TBSA showed a significant and direct association between them on the 1st (coefficient = 0.516; p = 0.001), 2nd (coefficient = 0.62; p <0.001), and 3rd (coefficient = 0.471; p = 0.002) day of follow up. Conclusion: According to this study, the redox perturbation occurred in burn injury which was measured and proved by decreased GSH/GSSG ratio as well as the shift of PAB in favour of oxidants. Besides, since PAB positively correlated with the severity of dermal damage, it might suggest the application of antioxidants as a part of therapeutic protocol for which the dosage should be proportionate to the surface area of the damaged skin.http://journals.sbmu.ac.ir/emergency/article/view/21125Oxidative stressoxidantsantioxidantsGlutathioneBurns. |
| spellingShingle | Arash Beiraghi-Toosi Roya Askarian Faezeh Sadrabadi Haghighi Mohammad Safarian Fereshteh Kalantari Seyed Isaac Hashemy Burn-induced Oxidative Stress and Serum Glutathione Depletion; a Cross Sectional Study Emergency Oxidative stress oxidants antioxidants Glutathione Burns. |
| title | Burn-induced Oxidative Stress and Serum Glutathione Depletion; a Cross Sectional Study |
| title_full | Burn-induced Oxidative Stress and Serum Glutathione Depletion; a Cross Sectional Study |
| title_fullStr | Burn-induced Oxidative Stress and Serum Glutathione Depletion; a Cross Sectional Study |
| title_full_unstemmed | Burn-induced Oxidative Stress and Serum Glutathione Depletion; a Cross Sectional Study |
| title_short | Burn-induced Oxidative Stress and Serum Glutathione Depletion; a Cross Sectional Study |
| title_sort | burn induced oxidative stress and serum glutathione depletion a cross sectional study |
| topic | Oxidative stress oxidants antioxidants Glutathione Burns. |
| url | http://journals.sbmu.ac.ir/emergency/article/view/21125 |
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