Performance of Qualitative and Quantitative Antigen Tests for SARS-CoV-2 Using Saliva
The rapid detection of SARS-CoV-2 is critical for the prevention of disease outbreaks. Antigen tests such as immunochromatographic assay (ICA) and chemiluminescent enzyme immunoassay (CLEIA) can yield results more quickly than PCR. We evaluated the performance of ICA and CLEIA using 34 frozen PCR-po...
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MDPI AG
2021-08-01
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| Series: | Infectious Disease Reports |
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| Online Access: | https://www.mdpi.com/2036-7449/13/3/69 |
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| author | Isao Yokota Takayo Sakurazawa Junichi Sugita Sumio Iwasaki Keiko Yasuda Naoki Yamashita Shinichi Fujisawa Mutsumi Nishida Satoshi Konno Takanori Teshima |
| author_facet | Isao Yokota Takayo Sakurazawa Junichi Sugita Sumio Iwasaki Keiko Yasuda Naoki Yamashita Shinichi Fujisawa Mutsumi Nishida Satoshi Konno Takanori Teshima |
| author_sort | Isao Yokota |
| collection | DOAJ |
| description | The rapid detection of SARS-CoV-2 is critical for the prevention of disease outbreaks. Antigen tests such as immunochromatographic assay (ICA) and chemiluminescent enzyme immunoassay (CLEIA) can yield results more quickly than PCR. We evaluated the performance of ICA and CLEIA using 34 frozen PCR-positive (17 saliva samples and 17 nasopharyngeal swabs [NPS]) and 309 PCR-negative samples. ICA detected SARS-CoV-2 in only 14 (41%) samples, with positivity rates of 24% in saliva and 59% in NPS. Notably, ICA detected SARS-CoV-2 in 5 of 6 samples collected within 4 days after symptom onset. CLEIA detected SARS-CoV-2 in 31 (91%) samples, with a positivity of 82% in saliva and 100% in NPS. These results suggest that the use of ICA should be limited to an earlier time after symptom onset and CLEIA is more sensitive and can be used in situations where quick results are required. |
| first_indexed | 2024-03-10T07:36:39Z |
| format | Article |
| id | doaj.art-501b93e2e1b34ebfb8c15625dbe21b4a |
| institution | Directory Open Access Journal |
| issn | 2036-7449 |
| language | English |
| last_indexed | 2024-03-10T07:36:39Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Infectious Disease Reports |
| spelling | doaj.art-501b93e2e1b34ebfb8c15625dbe21b4a2023-11-22T13:23:09ZengMDPI AGInfectious Disease Reports2036-74492021-08-0113374274710.3390/idr13030069Performance of Qualitative and Quantitative Antigen Tests for SARS-CoV-2 Using SalivaIsao Yokota0Takayo Sakurazawa1Junichi Sugita2Sumio Iwasaki3Keiko Yasuda4Naoki Yamashita5Shinichi Fujisawa6Mutsumi Nishida7Satoshi Konno8Takanori Teshima9Department of Biostatistics, Faculty of Medicine, Hokkaido University, Sapporo 060-8638, JapanDivision of Laboratory and Transfusion Medicine, Hokkaido University Hospital, N15, W7, Kita-ku, Sapporo 060-8638, JapanDivision of Laboratory and Transfusion Medicine, Hokkaido University Hospital, N15, W7, Kita-ku, Sapporo 060-8638, JapanDivision of Laboratory and Transfusion Medicine, Hokkaido University Hospital, N15, W7, Kita-ku, Sapporo 060-8638, JapanDivision of Laboratory and Transfusion Medicine, Hokkaido University Hospital, N15, W7, Kita-ku, Sapporo 060-8638, JapanDivision of Laboratory and Transfusion Medicine, Hokkaido University Hospital, N15, W7, Kita-ku, Sapporo 060-8638, JapanDivision of Laboratory and Transfusion Medicine, Hokkaido University Hospital, N15, W7, Kita-ku, Sapporo 060-8638, JapanDivision of Laboratory and Transfusion Medicine, Hokkaido University Hospital, N15, W7, Kita-ku, Sapporo 060-8638, JapanDepartment of Respiratory Medicine, Faculty of Medicine, Hokkaido University, Sapporo 060-8638, JapanDivision of Laboratory and Transfusion Medicine, Hokkaido University Hospital, N15, W7, Kita-ku, Sapporo 060-8638, JapanThe rapid detection of SARS-CoV-2 is critical for the prevention of disease outbreaks. Antigen tests such as immunochromatographic assay (ICA) and chemiluminescent enzyme immunoassay (CLEIA) can yield results more quickly than PCR. We evaluated the performance of ICA and CLEIA using 34 frozen PCR-positive (17 saliva samples and 17 nasopharyngeal swabs [NPS]) and 309 PCR-negative samples. ICA detected SARS-CoV-2 in only 14 (41%) samples, with positivity rates of 24% in saliva and 59% in NPS. Notably, ICA detected SARS-CoV-2 in 5 of 6 samples collected within 4 days after symptom onset. CLEIA detected SARS-CoV-2 in 31 (91%) samples, with a positivity of 82% in saliva and 100% in NPS. These results suggest that the use of ICA should be limited to an earlier time after symptom onset and CLEIA is more sensitive and can be used in situations where quick results are required.https://www.mdpi.com/2036-7449/13/3/69SARS-CoV-2COVID-19salivaLumipulseEsplineICA |
| spellingShingle | Isao Yokota Takayo Sakurazawa Junichi Sugita Sumio Iwasaki Keiko Yasuda Naoki Yamashita Shinichi Fujisawa Mutsumi Nishida Satoshi Konno Takanori Teshima Performance of Qualitative and Quantitative Antigen Tests for SARS-CoV-2 Using Saliva Infectious Disease Reports SARS-CoV-2 COVID-19 saliva Lumipulse Espline ICA |
| title | Performance of Qualitative and Quantitative Antigen Tests for SARS-CoV-2 Using Saliva |
| title_full | Performance of Qualitative and Quantitative Antigen Tests for SARS-CoV-2 Using Saliva |
| title_fullStr | Performance of Qualitative and Quantitative Antigen Tests for SARS-CoV-2 Using Saliva |
| title_full_unstemmed | Performance of Qualitative and Quantitative Antigen Tests for SARS-CoV-2 Using Saliva |
| title_short | Performance of Qualitative and Quantitative Antigen Tests for SARS-CoV-2 Using Saliva |
| title_sort | performance of qualitative and quantitative antigen tests for sars cov 2 using saliva |
| topic | SARS-CoV-2 COVID-19 saliva Lumipulse Espline ICA |
| url | https://www.mdpi.com/2036-7449/13/3/69 |
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