First Report of Domoic Acid Production from <i>Pseudo-nitzschia multistriata</i> in Paracas Bay (Peru)
The Peruvian sea is one of the most productive ecosystems in the world. Phytoplankton production provides food for fish, mammals, mollusks and birds. This trophic network is affected by the presence of toxic phytoplankton species. In July 2017, samples of phytoplankton were obtained from Paracas Bay...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/13/6/408 |
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| author | Cecil Tenorio Gonzalo Álvarez Sonia Quijano-Scheggia Melissa Perez-Alania Natalia Arakaki Michael Araya Francisco Álvarez Juan Blanco Eduardo Uribe |
| author_facet | Cecil Tenorio Gonzalo Álvarez Sonia Quijano-Scheggia Melissa Perez-Alania Natalia Arakaki Michael Araya Francisco Álvarez Juan Blanco Eduardo Uribe |
| author_sort | Cecil Tenorio |
| collection | DOAJ |
| description | The Peruvian sea is one of the most productive ecosystems in the world. Phytoplankton production provides food for fish, mammals, mollusks and birds. This trophic network is affected by the presence of toxic phytoplankton species. In July 2017, samples of phytoplankton were obtained from Paracas Bay, an important zone for scallop (<i>Argopecten purpuratus</i>) aquaculture in Peru. Morphological analysis revealed the presence of the genus <i>Pseudo-nitzschia</i>, which was isolated and cultivated in laboratory conditions. Subsequently, the monoclonal cultures were observed by scanning electron microscopy (SEM), and identified as <i>P. multistriata</i>, based on both the morphological characteristics, and internal transcribed spacers region (ITS2) sequence phylogenetic analysis. Toxin analysis using liquid chromatography (LC) with high-resolution mass spectrometry (HRMS) revealed the presence of domoic acid (DA) with an estimated amount of 0.004 to 0.010 pg cell<sup>−1</sup>. This is the first report of DA from the coastal waters of Peru and its detection in <i>P. multistriata</i> indicates that it is a potential risk. Based on our results, routine monitoring of this genus should be considered in order to ensure public health. |
| first_indexed | 2024-03-10T10:34:03Z |
| format | Article |
| id | doaj.art-50242913120245c095f45818b0f23947 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T10:34:03Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-50242913120245c095f45818b0f239472023-11-21T23:24:49ZengMDPI AGToxins2072-66512021-06-0113640810.3390/toxins13060408First Report of Domoic Acid Production from <i>Pseudo-nitzschia multistriata</i> in Paracas Bay (Peru)Cecil Tenorio0Gonzalo Álvarez1Sonia Quijano-Scheggia2Melissa Perez-Alania3Natalia Arakaki4Michael Araya5Francisco Álvarez6Juan Blanco7Eduardo Uribe8Banco de Germoplasma de Organismos Acuáticos, Instituto del Mar del Perú, Callao 07021, PeruDoctorado en Acuicultura, Programa Cooperativo Universidad de Chile, Pontificia Universidad Católica de Valparaíso, Coquimbo 17811421, ChileCentro Universitario de Investigaciones Oceanológicas, Universidad de Colima, Manzanillo 28218, MexicoFacultad de Ciencias, Universidad Nacional Agraria La Molina, Avenida La Universidad s/n, La Molina, Lima 15024, PeruBanco de Germoplasma de Organismos Acuáticos, Instituto del Mar del Perú, Callao 07021, PeruCentro de Investigación y Desarrollo Tecnológico en Algas (CIDTA), Facultad de Ciencias del Mar, Universidad Católica del Norte, Coquimbo 1281, ChileFacultad de Ciencias del Mar, Departamento de Acuicultura, Universidad Católica del Norte, Coquimbo 1281, ChileCentro de Investigacións Mariñas (Xunta de Galicia), 36620 Vilanova de Arousa, Pontevedra, SpainDoctorado en Acuicultura, Programa Cooperativo Universidad de Chile, Pontificia Universidad Católica de Valparaíso, Coquimbo 17811421, ChileThe Peruvian sea is one of the most productive ecosystems in the world. Phytoplankton production provides food for fish, mammals, mollusks and birds. This trophic network is affected by the presence of toxic phytoplankton species. In July 2017, samples of phytoplankton were obtained from Paracas Bay, an important zone for scallop (<i>Argopecten purpuratus</i>) aquaculture in Peru. Morphological analysis revealed the presence of the genus <i>Pseudo-nitzschia</i>, which was isolated and cultivated in laboratory conditions. Subsequently, the monoclonal cultures were observed by scanning electron microscopy (SEM), and identified as <i>P. multistriata</i>, based on both the morphological characteristics, and internal transcribed spacers region (ITS2) sequence phylogenetic analysis. Toxin analysis using liquid chromatography (LC) with high-resolution mass spectrometry (HRMS) revealed the presence of domoic acid (DA) with an estimated amount of 0.004 to 0.010 pg cell<sup>−1</sup>. This is the first report of DA from the coastal waters of Peru and its detection in <i>P. multistriata</i> indicates that it is a potential risk. Based on our results, routine monitoring of this genus should be considered in order to ensure public health.https://www.mdpi.com/2072-6651/13/6/408harmful algaeamnesic shellfish poisoningITS2<i>Argopecten purpuratus</i>scallop |
| spellingShingle | Cecil Tenorio Gonzalo Álvarez Sonia Quijano-Scheggia Melissa Perez-Alania Natalia Arakaki Michael Araya Francisco Álvarez Juan Blanco Eduardo Uribe First Report of Domoic Acid Production from <i>Pseudo-nitzschia multistriata</i> in Paracas Bay (Peru) Toxins harmful algae amnesic shellfish poisoning ITS2 <i>Argopecten purpuratus</i> scallop |
| title | First Report of Domoic Acid Production from <i>Pseudo-nitzschia multistriata</i> in Paracas Bay (Peru) |
| title_full | First Report of Domoic Acid Production from <i>Pseudo-nitzschia multistriata</i> in Paracas Bay (Peru) |
| title_fullStr | First Report of Domoic Acid Production from <i>Pseudo-nitzschia multistriata</i> in Paracas Bay (Peru) |
| title_full_unstemmed | First Report of Domoic Acid Production from <i>Pseudo-nitzschia multistriata</i> in Paracas Bay (Peru) |
| title_short | First Report of Domoic Acid Production from <i>Pseudo-nitzschia multistriata</i> in Paracas Bay (Peru) |
| title_sort | first report of domoic acid production from i pseudo nitzschia multistriata i in paracas bay peru |
| topic | harmful algae amnesic shellfish poisoning ITS2 <i>Argopecten purpuratus</i> scallop |
| url | https://www.mdpi.com/2072-6651/13/6/408 |
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