Exploring the Role of Epicardial Adipose Tissue in Coronary Artery Disease From the Difference of Gene Expression
ObjectivesEpicardial adipose tissue (EAT) is closely adjacent to the coronary arteries and myocardium, its role as an endocrine organ to affect the pathophysiological processes of the coronary arteries and myocardium has been increasingly recognized. However, the specific gene expression profiles of...
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2021.605811/full |
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| author | Qian-Chen Wang Zhen-Yu Wang Qian Xu Ruo-Bing Li Guo-Gang Zhang Rui-Zheng Shi |
| author_facet | Qian-Chen Wang Zhen-Yu Wang Qian Xu Ruo-Bing Li Guo-Gang Zhang Rui-Zheng Shi |
| author_sort | Qian-Chen Wang |
| collection | DOAJ |
| description | ObjectivesEpicardial adipose tissue (EAT) is closely adjacent to the coronary arteries and myocardium, its role as an endocrine organ to affect the pathophysiological processes of the coronary arteries and myocardium has been increasingly recognized. However, the specific gene expression profiles of EAT in coronary artery disease (CAD) has not been well characterized. Our aim was to investigate the role of EAT in CAD at the gene level.MethodsHere, we compared the histological and gene expression difference of EAT between CAD and non-CAD. We investigated the gene expression profiles in the EAT of patients with CAD through the high-throughput RNA sequencing. We performed bioinformatics analysis such as functional enrichment analysis and protein-protein interaction network construction to obtain and verify the hub differentially expressed genes (DEGs) in the EAT of CAD.ResultsOur results showed that the size of epicardial adipocytes in the CAD group was larger than in the control group. Our findings on the EAT gene expression profiles of CAD showed a total of 747 DEGs (fold change >2, p value <0.05). The enrichment analysis of DEGs showed that more pro-inflammatory and immunological genes and pathways were involved in CAD. Ten hub DEGs (GNG3, MCHR1, BDKRB1, MCHR2, CXCL8, CXCR5, CCR8, CCL4L1, TAS2R10, and TAS2R41) were identified.ConclusionEpicardial adipose tissue in CAD shows unique gene expression profiles and may act as key regulators in the CAD pathological process. |
| first_indexed | 2024-12-13T18:36:32Z |
| format | Article |
| id | doaj.art-502cf073bfe64d8cb4d5907ac92d696e |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-13T18:36:32Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-502cf073bfe64d8cb4d5907ac92d696e2022-12-21T23:35:21ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2021-03-011210.3389/fphys.2021.605811605811Exploring the Role of Epicardial Adipose Tissue in Coronary Artery Disease From the Difference of Gene ExpressionQian-Chen Wang0Zhen-Yu Wang1Qian Xu2Ruo-Bing Li3Guo-Gang Zhang4Rui-Zheng Shi5Department of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Cardiovascular Medicine, The Second Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Cardiovascular Surgery, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha, ChinaObjectivesEpicardial adipose tissue (EAT) is closely adjacent to the coronary arteries and myocardium, its role as an endocrine organ to affect the pathophysiological processes of the coronary arteries and myocardium has been increasingly recognized. However, the specific gene expression profiles of EAT in coronary artery disease (CAD) has not been well characterized. Our aim was to investigate the role of EAT in CAD at the gene level.MethodsHere, we compared the histological and gene expression difference of EAT between CAD and non-CAD. We investigated the gene expression profiles in the EAT of patients with CAD through the high-throughput RNA sequencing. We performed bioinformatics analysis such as functional enrichment analysis and protein-protein interaction network construction to obtain and verify the hub differentially expressed genes (DEGs) in the EAT of CAD.ResultsOur results showed that the size of epicardial adipocytes in the CAD group was larger than in the control group. Our findings on the EAT gene expression profiles of CAD showed a total of 747 DEGs (fold change >2, p value <0.05). The enrichment analysis of DEGs showed that more pro-inflammatory and immunological genes and pathways were involved in CAD. Ten hub DEGs (GNG3, MCHR1, BDKRB1, MCHR2, CXCL8, CXCR5, CCR8, CCL4L1, TAS2R10, and TAS2R41) were identified.ConclusionEpicardial adipose tissue in CAD shows unique gene expression profiles and may act as key regulators in the CAD pathological process.https://www.frontiersin.org/articles/10.3389/fphys.2021.605811/fullepicardial adipose tissuecoronary artery diseasegene expression profilesbioinformatics analysismRNA |
| spellingShingle | Qian-Chen Wang Zhen-Yu Wang Qian Xu Ruo-Bing Li Guo-Gang Zhang Rui-Zheng Shi Exploring the Role of Epicardial Adipose Tissue in Coronary Artery Disease From the Difference of Gene Expression Frontiers in Physiology epicardial adipose tissue coronary artery disease gene expression profiles bioinformatics analysis mRNA |
| title | Exploring the Role of Epicardial Adipose Tissue in Coronary Artery Disease From the Difference of Gene Expression |
| title_full | Exploring the Role of Epicardial Adipose Tissue in Coronary Artery Disease From the Difference of Gene Expression |
| title_fullStr | Exploring the Role of Epicardial Adipose Tissue in Coronary Artery Disease From the Difference of Gene Expression |
| title_full_unstemmed | Exploring the Role of Epicardial Adipose Tissue in Coronary Artery Disease From the Difference of Gene Expression |
| title_short | Exploring the Role of Epicardial Adipose Tissue in Coronary Artery Disease From the Difference of Gene Expression |
| title_sort | exploring the role of epicardial adipose tissue in coronary artery disease from the difference of gene expression |
| topic | epicardial adipose tissue coronary artery disease gene expression profiles bioinformatics analysis mRNA |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2021.605811/full |
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