Occurrence of target spot of tomato caused by <i>Corynespora cassiicola</i> in Sri Lanka
In February 2019, a new destructive leaf and stem spot disease was reported from commercial tomato fields in Kimbissa, Central Province, Sri Lanka. This disease resulted in significant loss of foliage where the disease severity ranged from 50-70% in the infected tomato fields. The objectives of the...
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| Format: | Article |
| Language: | English |
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Faculty of Science, University of Peradeniya, Sri Lanka
2020-11-01
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| Series: | Ceylon Journal of Science |
| Subjects: | |
| Online Access: | https://cjs.sljol.info/articles/7807 |
| _version_ | 1811325053162225664 |
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| author | W. A. P. G. Weeraratne W. M. S. D. K. Wijerathne D. M. K. K. Dissanayake |
| author_facet | W. A. P. G. Weeraratne W. M. S. D. K. Wijerathne D. M. K. K. Dissanayake |
| author_sort | W. A. P. G. Weeraratne |
| collection | DOAJ |
| description | In February 2019, a new destructive leaf and stem spot disease was reported from commercial tomato fields in Kimbissa, Central Province, Sri Lanka. This disease resulted in significant loss of foliage where the disease severity ranged from 50-70% in the infected tomato fields. The objectives of the study were to isolate and characterize the pathogen causing the disease and confirm its pathogenicity. A fungal pathogen with cottony, grayish mycelium and cylindrical, straight, multi-septate conidia was isolated from the leaf and stem lesions on Potato Dextrose Agar PDA. Based on morphological, molecular and pathological data, the fungus was identified as <em>Corynespora cassiicola</em>. Re-inoculation of leaves and stems of healthy tomato plants under green house conditions produced symptoms similar to those observed in the field. Sequence was deposited in GenBank for Sri Lankan <em>C. cassiicola</em> strain TS-1 (Accession No.MT071510.1). A BLAST search revealed 97% sequence identity to <em>Corynespora cassiicola</em> in GenBank Accession No. MH780759.1 (Soybean, Brazil). |
| first_indexed | 2024-04-13T14:26:14Z |
| format | Article |
| id | doaj.art-5030a3c471d44ed8abbf0890d8364207 |
| institution | Directory Open Access Journal |
| issn | 2513-2814 2513-230X |
| language | English |
| last_indexed | 2024-04-13T14:26:14Z |
| publishDate | 2020-11-01 |
| publisher | Faculty of Science, University of Peradeniya, Sri Lanka |
| record_format | Article |
| series | Ceylon Journal of Science |
| spelling | doaj.art-5030a3c471d44ed8abbf0890d83642072022-12-22T02:43:18ZengFaculty of Science, University of Peradeniya, Sri LankaCeylon Journal of Science2513-28142513-230X2020-11-0149539740010.4038/cjs.v49i5.78075832Occurrence of target spot of tomato caused by <i>Corynespora cassiicola</i> in Sri LankaW. A. P. G. Weeraratne0W. M. S. D. K. Wijerathne1D. M. K. K. Dissanayake2Horticultural Crop Research and Development Institute, Gannoruwa, PeradeniyaHorticultural Crop Research and Development Institute, Gannoruwa, PeradeniyaHorticultural Crop Research and Development Institute, Gannoruwa, PeradeniyaIn February 2019, a new destructive leaf and stem spot disease was reported from commercial tomato fields in Kimbissa, Central Province, Sri Lanka. This disease resulted in significant loss of foliage where the disease severity ranged from 50-70% in the infected tomato fields. The objectives of the study were to isolate and characterize the pathogen causing the disease and confirm its pathogenicity. A fungal pathogen with cottony, grayish mycelium and cylindrical, straight, multi-septate conidia was isolated from the leaf and stem lesions on Potato Dextrose Agar PDA. Based on morphological, molecular and pathological data, the fungus was identified as <em>Corynespora cassiicola</em>. Re-inoculation of leaves and stems of healthy tomato plants under green house conditions produced symptoms similar to those observed in the field. Sequence was deposited in GenBank for Sri Lankan <em>C. cassiicola</em> strain TS-1 (Accession No.MT071510.1). A BLAST search revealed 97% sequence identity to <em>Corynespora cassiicola</em> in GenBank Accession No. MH780759.1 (Soybean, Brazil).https://cjs.sljol.info/articles/7807molecular identification, target spot, tomato |
| spellingShingle | W. A. P. G. Weeraratne W. M. S. D. K. Wijerathne D. M. K. K. Dissanayake Occurrence of target spot of tomato caused by <i>Corynespora cassiicola</i> in Sri Lanka Ceylon Journal of Science molecular identification, target spot, tomato |
| title | Occurrence of target spot of tomato caused by <i>Corynespora cassiicola</i> in Sri Lanka |
| title_full | Occurrence of target spot of tomato caused by <i>Corynespora cassiicola</i> in Sri Lanka |
| title_fullStr | Occurrence of target spot of tomato caused by <i>Corynespora cassiicola</i> in Sri Lanka |
| title_full_unstemmed | Occurrence of target spot of tomato caused by <i>Corynespora cassiicola</i> in Sri Lanka |
| title_short | Occurrence of target spot of tomato caused by <i>Corynespora cassiicola</i> in Sri Lanka |
| title_sort | occurrence of target spot of tomato caused by i corynespora cassiicola i in sri lanka |
| topic | molecular identification, target spot, tomato |
| url | https://cjs.sljol.info/articles/7807 |
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