| Summary: | The Sarcophagidae family of fresh flies bears strong importance in the context of forensic entomology due to their application in the estimation of the Post Mortem Interval (PMI). Sarcophaga is the major genus in the Sarcophagidae family and includes cosmopolitan species, which are distributed worldwide. In this communication, we present the analysis of the complete mitochondrial genome (mtDNA) of two species from Portugal – S. melanura and S. dux – and one from Brazil – S. ruficornis. The mtDNA of these species range from 14,882 bp to 15,190 bp and have 22 tRNA genes, 13 protein-coding genes (PCG), and two rRNAs distributed along both strands. Our data include the first record of complete Sarcophaga mtDNA sequences from species collected in Portugal and in Brazil. These genomes represent an advance in the understanding about this group, expand the database, and can be used for the development of new markers for species identification.
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