The Presence of Plant-Associated Bacteria Alters Responses to <i>N</i>-acyl Homoserine Lactone Quorum Sensing Signals that Modulate Nodulation in <i>Medicago Truncatula</i>
Bacteria use quorum sensing signaling for cell-to-cell communication, which is also important for their interactions with plant hosts. Quorum sensing via <i>N</i>-acyl-homoserine lactones (AHLs) is important for successful symbioses between legumes and nitrogen-fixing rhizobia. Previous...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-06-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/9/6/777 |
| _version_ | 1797564505708822528 |
|---|---|
| author | Debora F. Veliz-Vallejos Akitomo Kawasaki Ulrike Mathesius |
| author_facet | Debora F. Veliz-Vallejos Akitomo Kawasaki Ulrike Mathesius |
| author_sort | Debora F. Veliz-Vallejos |
| collection | DOAJ |
| description | Bacteria use quorum sensing signaling for cell-to-cell communication, which is also important for their interactions with plant hosts. Quorum sensing via <i>N</i>-acyl-homoserine lactones (AHLs) is important for successful symbioses between legumes and nitrogen-fixing rhizobia. Previous studies have shown that plant hosts can recognize and respond to AHLs. Here, we tested whether the response of the model legume <i>Medicago truncatula</i> to AHLs from its symbiont and other bacteria could be modulated by the abundance and composition of plant-associated microbial communities. Temporary antibiotic treatment of the seeds removed the majority of bacterial taxa associated with <i>M. truncatula</i> roots and significantly altered the effect of AHLs on nodule numbers, but lateral root density, biomass, and root length responses were much less affected. The AHL 3-oxo-C<sub>14</sub>-HSL (homoserine lactone) specifically increased nodule numbers but only after the treatment of seeds with antibiotics. This increase was associated with increased expression of the early nodulation genes <i>RIP1</i> and <i>ENOD11</i> at 24 h after infection. A 454 pyrosequencing analysis of the plant-associated bacteria showed that antibiotic treatment had the biggest effect on bacterial community composition. However, we also found distinct effects of 3-oxo-C<sub>14</sub>-HSL on the abundance of specific bacterial taxa. Our results revealed a complex interaction between plants and their associated microbiome that could modify plant responses to AHLs. |
| first_indexed | 2024-03-10T18:58:10Z |
| format | Article |
| id | doaj.art-50354cc1405a4348b85f0e9ff007f844 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T18:58:10Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-50354cc1405a4348b85f0e9ff007f8442023-11-20T04:34:43ZengMDPI AGPlants2223-77472020-06-019677710.3390/plants9060777The Presence of Plant-Associated Bacteria Alters Responses to <i>N</i>-acyl Homoserine Lactone Quorum Sensing Signals that Modulate Nodulation in <i>Medicago Truncatula</i>Debora F. Veliz-Vallejos0Akitomo Kawasaki1Ulrike Mathesius2Division of Plant Sciences, Research School of Biology, Canberra, ACT 2601, AustraliaDivision of Plant Sciences, Research School of Biology, Canberra, ACT 2601, AustraliaDivision of Plant Sciences, Research School of Biology, Canberra, ACT 2601, AustraliaBacteria use quorum sensing signaling for cell-to-cell communication, which is also important for their interactions with plant hosts. Quorum sensing via <i>N</i>-acyl-homoserine lactones (AHLs) is important for successful symbioses between legumes and nitrogen-fixing rhizobia. Previous studies have shown that plant hosts can recognize and respond to AHLs. Here, we tested whether the response of the model legume <i>Medicago truncatula</i> to AHLs from its symbiont and other bacteria could be modulated by the abundance and composition of plant-associated microbial communities. Temporary antibiotic treatment of the seeds removed the majority of bacterial taxa associated with <i>M. truncatula</i> roots and significantly altered the effect of AHLs on nodule numbers, but lateral root density, biomass, and root length responses were much less affected. The AHL 3-oxo-C<sub>14</sub>-HSL (homoserine lactone) specifically increased nodule numbers but only after the treatment of seeds with antibiotics. This increase was associated with increased expression of the early nodulation genes <i>RIP1</i> and <i>ENOD11</i> at 24 h after infection. A 454 pyrosequencing analysis of the plant-associated bacteria showed that antibiotic treatment had the biggest effect on bacterial community composition. However, we also found distinct effects of 3-oxo-C<sub>14</sub>-HSL on the abundance of specific bacterial taxa. Our results revealed a complex interaction between plants and their associated microbiome that could modify plant responses to AHLs.https://www.mdpi.com/2223-7747/9/6/777acyl-homoserine lactoneslegumemicrobiomenodulationrhizobiaquorum sensing |
| spellingShingle | Debora F. Veliz-Vallejos Akitomo Kawasaki Ulrike Mathesius The Presence of Plant-Associated Bacteria Alters Responses to <i>N</i>-acyl Homoserine Lactone Quorum Sensing Signals that Modulate Nodulation in <i>Medicago Truncatula</i> Plants acyl-homoserine lactones legume microbiome nodulation rhizobia quorum sensing |
| title | The Presence of Plant-Associated Bacteria Alters Responses to <i>N</i>-acyl Homoserine Lactone Quorum Sensing Signals that Modulate Nodulation in <i>Medicago Truncatula</i> |
| title_full | The Presence of Plant-Associated Bacteria Alters Responses to <i>N</i>-acyl Homoserine Lactone Quorum Sensing Signals that Modulate Nodulation in <i>Medicago Truncatula</i> |
| title_fullStr | The Presence of Plant-Associated Bacteria Alters Responses to <i>N</i>-acyl Homoserine Lactone Quorum Sensing Signals that Modulate Nodulation in <i>Medicago Truncatula</i> |
| title_full_unstemmed | The Presence of Plant-Associated Bacteria Alters Responses to <i>N</i>-acyl Homoserine Lactone Quorum Sensing Signals that Modulate Nodulation in <i>Medicago Truncatula</i> |
| title_short | The Presence of Plant-Associated Bacteria Alters Responses to <i>N</i>-acyl Homoserine Lactone Quorum Sensing Signals that Modulate Nodulation in <i>Medicago Truncatula</i> |
| title_sort | presence of plant associated bacteria alters responses to i n i acyl homoserine lactone quorum sensing signals that modulate nodulation in i medicago truncatula i |
| topic | acyl-homoserine lactones legume microbiome nodulation rhizobia quorum sensing |
| url | https://www.mdpi.com/2223-7747/9/6/777 |
| work_keys_str_mv | AT deborafvelizvallejos thepresenceofplantassociatedbacteriaaltersresponsestoiniacylhomoserinelactonequorumsensingsignalsthatmodulatenodulationinimedicagotruncatulai AT akitomokawasaki thepresenceofplantassociatedbacteriaaltersresponsestoiniacylhomoserinelactonequorumsensingsignalsthatmodulatenodulationinimedicagotruncatulai AT ulrikemathesius thepresenceofplantassociatedbacteriaaltersresponsestoiniacylhomoserinelactonequorumsensingsignalsthatmodulatenodulationinimedicagotruncatulai AT deborafvelizvallejos presenceofplantassociatedbacteriaaltersresponsestoiniacylhomoserinelactonequorumsensingsignalsthatmodulatenodulationinimedicagotruncatulai AT akitomokawasaki presenceofplantassociatedbacteriaaltersresponsestoiniacylhomoserinelactonequorumsensingsignalsthatmodulatenodulationinimedicagotruncatulai AT ulrikemathesius presenceofplantassociatedbacteriaaltersresponsestoiniacylhomoserinelactonequorumsensingsignalsthatmodulatenodulationinimedicagotruncatulai |